Mycotrim® RS - Irvine Scientific

B. Colony appearance
Do not use an inverted microscope.
When a color change occurs within the first 72 hours, colonies
are usually visible microscopically. A conventional light
microscope with a low power (10x) objective lens and 10x
eyepiece, or a good quality dissecting microscope with 70x to
100x magnifying capability is adequate. It will be necessary
to adjust the stage or nosepiece of the microscope to accommodate
the depth of the Mycotrim RS flask.
In specimens with very high or very low titers of ureaplasma,
it may be possible to observe a color change and be unable to
see microscopic colonies.
To examine the flask for M. pneumoniae colonies:
1. Place the flask, agar side up, on the microscope stage as
indicated in Figure 3. Focus through the flask and agar
to the inside surface of the agar. The cut made during
inoculation marks the area inoculated and aids in focusing.
2. Scan the length of the flask along both sides of the cut for
colonies.
AGAR
BROTH
10x
Figure 3
3. Examine suspected colonies by focusing up and down
on them . Colonies may grow on the agar surface or
penetrate below the agar surface. Colonies growing in the
agar may not appear in sharp focus.
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