Mycotrim® RS - Irvine Scientific

3. As the swab is withdrawn from the flask, carefully press it
into the agar to make a deep cut in the center of the inoculated
area. The swab will not make a clean cut; however,
a jagged cut will not adversely affect the performance of
Mycotrim RS.
4. Recap the flask tightly.
3. Incubation
Incubate the flask agar side up at 34° C to 37° C for 72 hours
or until the color of the media changes. Check the flask for a
color change every 24 hours (see 4. Observation, below).
Temperatures above 38° C will inhibit M. pneumoniae.
When removing flasks from the incubator for examination,
care should be taken to avoid washing growth off the agar by
splashing the broth over the agar.
4. Observation
A. Color change
The first sign of M. pneumoniae in Mycotrim RS is a color
change in the media which may occur within 72 to 96 hours
after inoculation. Growth of M. pneumoniae will cause the
media to change from red to yellow-orange.
Unlike bacteria and fungi, M. pneumoniae does not cause
turbidity in the broth, even at maximum titers. Gross turbidity
of visible colonies on the agar indicate bacterial or fungal
overgrowth, making the color change unreliable and possibly
hiding mycoplasma colonies. If no color change occurs
within 72 hours, reinoculate the agar as indicated in step 5.
Reinoculation, below.
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