Mycotrim® RS - Irvine Scientific

DIRECTIONS FOR USE
1. Preparation of Flask
A. Loosen the flask cap 1/4 to 1/2 turn and warm the flask
in the incubator (34° C to 37° C) for approximately five
minutes.
B. Aseptically add antimicrobial discs according to specimen
type:
Specimen Source Antimicrobial Discs
Throat 1 CN, 1 TA
Bronchial washings 1 CN, 1 TA
Pneumocentesis fluid 1 CN, 1 TA
Tissue from biopsy 1 CN, 1 TA
Sputum 2 CN, 2 TA
C. Recap the flask tightly and place it agar side down, as
shown below in Figure 1, for a minimum of 30 minutes.
BROTH
AGAR
Figure 1
2. Inoculation
Use aseptic technique.
A. For liquid specimens such as bronchial washings, pneumocentesis
fluid and washings of tissue biopsy:
1. Hold the flask at about a 45° angle as shown in Figure 2
and allow approximately 1.0 mL of specimen to slow flow
down the center of the agar.
2. As the pipet is withdrawn from the flask, press it firmly into
the agar to make a deep cut in the area inoculated.
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