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Mycotrim® RS - Irvine Scientific

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Mycotrim <strong>RS</strong> kit components should be used before the expiration<br />

date printed on the box.<br />

MATERIALS PROVIDED<br />

Component Qty per 12 Flask Kit<br />

Agar/Broth Flask 12<br />

Cefoperazone Sodium/Nystatin Discs 2 dishes (15 discs/dish)<br />

Thallous acetate 2 dishes (15 discs/dish)<br />

Dienes Stain 1 Vial (2.5 mL/vial)<br />

Antimicrobial Discs<br />

A single white CN antimicrobial disc yields an approximate<br />

concentration in agar and broth of:<br />

Cefoperazone sodium 100.0 μg/mL<br />

Nystatin 50.0 U/mL<br />

Each pink TA antimicrobial disc yields approximately:<br />

Thallous acetate 250.0 μg/mL<br />

Dienes stain - contains methylene blue and azure II.<br />

MATERIALS REQUIRED BUT NOT PROVIDED<br />

Sterile pipets, or<br />

Sterile swabs, or<br />

Sterile bacteriological loop<br />

Sterile forceps<br />

Conventional microscope with 10x eyepieces and 10x objective or<br />

dissecting microscope with 70x to 100x magnification.<br />

Incubator capable of maintaining temperatures of 34° C to 37° C.<br />

PRECAUTIONS AND WARNINGS<br />

Do not use if the Mycotrim <strong>RS</strong> flask is cracked or if the media<br />

shows signs of contamination. The broth should be clear and<br />

reddish-colored with no signs of cloudiness.<br />

English 2/10

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