Mycotrim® RS - Irvine Scientific
Mycotrim® RS - Irvine Scientific
Mycotrim® RS - Irvine Scientific
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INTENDED USE<br />
Mycotrim ® <strong>RS</strong> is a complete culture system for Mycoplasma pneumoniae<br />
(M. pneumoniae) in human respiratory specimens.<br />
Mycoplasma pneumoniae is a common cause of respiratory infections.<br />
M. pneumoniae is the cause of primary atypical pneumonia,<br />
often called “walking pneumonia”. It is associated with chronic<br />
bronchitis and is thought to account for more than 20% of all<br />
pneumonia cases.<br />
Mycotrim <strong>RS</strong> is designed to selectively isolate and identify M.<br />
pneumoniae in human respiratory specimens.<br />
PRODUCT DESCRIPTION<br />
Each Mycotrim <strong>RS</strong> flask contains agar and broth specially formulated<br />
to support the growth of M. pneumoniae. When the flask is<br />
inoculated and incubated with the agar side up, the agar and broth<br />
are separated by humid air. This creates optimal conditions for the<br />
growth of M. pneumoniae on the agar surface. Antimicrobial discs<br />
are added before inoculation to prevent the growth of microorganisms<br />
other than mycoplasma.<br />
Positive cultures of M. pneumoniae lower the pH and cause a<br />
color change in the media. In specimens with high titers, this<br />
color change may occur in as little as 72 to 96 hours, while in<br />
specimens with low titers it may take 7 to 10 days. Flasks showing<br />
no color change after 72 hours are reinoculated with the enrichment<br />
broth in the flask. Once the media changes color, the flask is<br />
examined microscopically (low power) for M. pneumoniae colonies<br />
on the agar surface.<br />
STORAGE AND STABILITY<br />
Store all the Mycotrim <strong>RS</strong> components (fl asks, antimicrobial discs and<br />
Dienes stain) in the original box at 2° C to 8° C.<br />
Do not freeze.<br />
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