Mycotrim® RS - Irvine Scientific

LIMITATIONS
The presence of very large numbers of organisms other than M.
pneumoniae may cause overgrowth even when antimicrobic discs
are used. Turbid broth or colonies visible to the naked eye indicate
overgrowth. Bacteria and fungi may cause a color change in the
absence of M. pneumoniae. Overgrowth may hide the presence of
M. pneumoniae colonies.
Antimicrobial effectiveness is compromised when the specimen
is inoculated before complete dispersion of the antimicrobials or
when the disc has been in the Mycotrim RS flask for more than 24
hours before it is inoculated.
QUALITY CONTROL
Each fl ask of Mycotrim RS is incubated and inspected for
contamination. Each lot of Mycotrim RS is tested for its ability to
maintain M. pneumoniae (ATCC #15531) and Acholeplasma laidlawii
(ATCC #23206). Growth of these organisms must be demonstrated
in both broth and agar media. The inhibitory activity of antimicrobial
discs is tested by inoculating fl asks containing antimicrobial discs
with Staphylococcus aureus (ATCC #25923), Escherichia coli (ATCC
#25922), and Candida albicans (ATCC #10231). Inhibition of all three
organisms must be shown after incubation for 24 hours at 34° C to 37° C.
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