Guía de referencia rápida Pseudalert* - Pàgines de la UAB
Guía de referencia rápida Pseudalert* - Pàgines de la UAB
Guía de referencia rápida Pseudalert* - Pàgines de la UAB
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Rev 03252011<br />
Technical Note<br />
Comparison of the performance of the IDEXX <strong>Pseudalert*</strong> test against the ISO 16266<br />
Method at recovering Pseudomonas aeruginosa from thermal pool and bottled water<br />
samples<br />
Scope<br />
i<strong>de</strong>xx.com<br />
This technical note contains data collected at the Nestlé Waters Quality Assurance Center<br />
in Vittel, France that evaluated the performance of the Pseudalert test prior to its <strong>la</strong>unch in<br />
September 2010. Samples of water used in the production of bottled water products and<br />
from thermal (mineral) pools/spas served as the test matrix for this study. The<br />
microorganisms present in these water samples were from wild popu<strong>la</strong>tions that occurred<br />
naturally in the environment and did not result from supplemental spiking activities.<br />
Additional water samples were collected that were spiked with different bacterial species to<br />
simu<strong>la</strong>te a contamination event. Testing occurred over the course of two months. This<br />
study compared the re<strong>la</strong>tive recovery of confirmed P. aeruginosa by Pseudalert after 24<br />
hours of incubation against the ISO 16266 method.<br />
Procedure<br />
1. Water samples (>500 mL) were collected for testing. Thermal pool water samples<br />
were analyzed the day of sampling and stored at room temperature. Bottled water<br />
samples were obtained from facilities located at multiple global locations. They were<br />
shipped and stored at room temperature prior to testing.<br />
2. A 250 mL aliquot of each sample was processed and analyzed following the<br />
procedures outlined in the ISO 16266 protocol.<br />
3. A 250 mL aliquot of each sample was processed and analyzed following the<br />
procedures outlined in the Pseudalert package insert for 250 mL presence/absence<br />
testing using an IDEXX testing vessel. Pseudalert was incubated for 24 hours at<br />
38±0.5ºC.<br />
4. Presumptive P. aeruginosa positive samples from the ISO 16266 method were<br />
subjected to the following confirmation procedures:<br />
• No further confirmation was required if the colony was blue/green on CN agar<br />
• Confirmation in acetami<strong>de</strong> broth was performed if the colony was not blue/green but<br />
fluorescent on CN agar<br />
• Confirmation in Oxidase, Kings B, and acetami<strong>de</strong> broth were performed if the colony<br />
was not fluorescent but reddish/brown color on CN agar<br />
5. Pseudalert positive samples were not confirmed.<br />
6. The heterotrophic count of each water sample was measured following the ISO 6222<br />
method.<br />
* Pseudalert is a tra<strong>de</strong>mark or registered tra<strong>de</strong>mark of IDEXX Laboratories, Inc. or its affiliates in the United States and/or<br />
other countries.