UNIVERSIDAD COMPLUTENSE DE MADRID - Biblioteca de la ...

Eduardo Gómez Casado
Material y Métodos 58
Tabla 7. Oligonucleótidos empleados en el oligotipaje del locus HLA-B.
URSTO a
Secuencia 5'!3'
Localización Especificidades del grupo CG Especificidades del grupo TA
4 #
CGG ATC GCG CTC CGC TAC 307-324 B*15(13,16,17,23,24),*2702,*4406,*4901,*57,*59 B*0803,*3801,*51,*52,*53,*58
5 #
TAC CTG GAG GGC CTG TGC 547-564 B*1303,*15,*4005,*41,*44(-04),*45,*49,*50,*56,*57 B*35,*4802,*51,*52,*53,*58
6 #
CAG AGG ATG TAT GGC TGC 358-375 B*1301,*15(02,13,16,20,21,25),*4004,*4101,
B*35(-05,12),*4802,*5104,*53,*58
*44032,*45,*49,*50
7 # ACA CCC TCC AGA GGA TGT 350-367 B*15(-02,04,07,13,16,20,21,25)1801,*40(01,07),*4601, B*1522,*38,*39(-03,06),*67
*47,*5602
8 # CAG AGG ATG TTT GGC TGC 358-375 B*1506,*47 ---
10 #
CTC ACA TCA TCC AGA GGA 347-364 B*1301,*15(02,13,20,21,25),*4004,*44 B*35(-05),*4802,*5104,*53,*5801
11 #
TGT ATG GCT GCG ACC TGG 365-382 B*13,*15(16,20,34),*4004,*4410,*45,*49,*50,*54, B*35(-05,12,16,17),*4802,*53,*58
*55(-04,06),*5601,*59,*8201
14 #
GCG GAC ACG GCG GCT CAG 478-495 B*15(-20),*27(-053),*3702,*40,*46,*47,*5603,*57,*73 B*7,*1522,*35(092,18),48(01,03),*81
15 #
GGA GCA GTG GAG AGC CTA 531-548 B*15(01,04,07,08,11,12,14,15,19,22,24,26N,28),*46 B*1522
20 # GGC CCG ACG GGC GCC TCC 382-400 B*1520,*4501,*49,*50 B*35(-02,12),*4802,*53(01,02),*58
22 #
TCC GCG GGC ATA ACC AGT 401-418 B*13,*15(09,30),*27(07,11),*40(-03,09),*41,*4410, B*7,*8,*35(02,04,06,09,12,18),*38,*39(-07),
*54,*55,*56(-03),*57(02,03),*59,*82
*42,*48(01,03),*51,*52,*67,*78,*81
23 #
ACC AGT TCG CCT ACG ACG 413-430 B*3701,*73 B*14,*35(03,06,13),*38,*39(-07),*67
24 #
ATT GGG ACC GGA ACA CAC 248-265 B*15(02,08,09,10,11,13,15,18,22,23,29),*18,*4008, B*7,*8,*14,*35(-10,13,16),*38,*39(-021,
*4406,*54,*55,*56,*59,*73,*82
022,08),*42,*51,*53,*67,*78,*8101
25 #
TAC CTG GAG GGC ACG TGC 557-574 B*18,*37,*41,*4404,*54,*55,*59 B*8,*14,*38,*39,*42,*67
26 #
TGT ATG GCT GCG ACG TGG 365-382 B*15(02,04,13,21,25),*1802,*27(-07,11),*4101,
B*14,*39062,*51(-06),*52,*78
*44(032,07)
30 # CGG AAC CTG CGC GGC TAC 307-324 --- B*7,*8(01,04),*14,*35,*39(-07),*42,*48,
*67,*78,*81
31 #
ATT TCT ACA CCT CCG TGT 92-109 --- B*7,*1401,*38,*39(-04,12),*4201,*48,*67,
*8101
32 # GCC CGT GTG GCG GAG CAG 520-537 --- B*8,*35(-11,*21),*38,*39,*42,*48,
*51(05,08),*53,*58,*67,*81
33 # GAT CTC CAA GAC CAA CAC 267-284 --- B*3520,*39(021,022,08),*48,*52,*5107
34 # TGA CCA GTC CGC CTA CGA 411-428 --- B*35(01,05,07,08,10,11,15,16,17,19,20,21),
*3907,*4802,*53,*58
35 #
AAC ACA CAG ATC TAC AAG 259-276 B*1511,*54,*55,*56 B*7,*3910,*42,*67,*8101
36 #
CGC GGG CGC CGT GGG TGG 212-229 B*54 ---
41 # ACC AAC ACA CAG ACT GAC 277-295 B*1803 B*0703,*8(-03),*14,*39(-05,07,08,11),*7801
C
42 # GGC GGA GCA GCG GAG A 528-543 B*4404 B*7(-04),*35(08,18),*39(08,11),*5105
43 #
CAG GAC GCC TAC GAC GGC 415-432 B*15(14,17),*27(-06,07,09),*3702,*44(-05),*47