TESIS FICOTOXINAS MARINAS EVA FONFRIA

ACA-07-2100. Supplementary material.
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Figure 2S. (A) Association curves of different YTX concentrations without subtraction of DMSO
controls and (B) association curves of their DMSO controls.
Figure 4S. PDE I immobilization. Activation of the carboxylic groups of CM5 chip was performed
with a mixture of EDC/NHS injected over the sensor surface for 10 min. Then, PDE I (0.25 mg/ml)
was added for 14 min. The remaining activated sites were then blocked with 1 M ethanolamine-HCl,
for 5 min. Finally, 10 mM HCl was injected for 1 min. Bound PDE I is indicated by the arrow.