TESIS FICOTOXINAS MARINAS EVA FONFRIA

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13-desmethyl C spirolide (nM) Gymnodimine (nM) Mussel Cockle Clam Scallop 74 Publicaciones 213.4 ± 21.2 163.6 ± 22.2 194.8 ± 12.7 160.2 ± 14.1 341.0 ± 73.3 294.4 ± 94.3 339.9 ± 74.0 359.2 ± 92.4 Table 3. Post-extraction contamination concentrations calculated of 13desmethyl C spirolide and GYM using calibration curves in shellfish extracts. Mussels, cockles, clams and scallops were extracted using the acetone/chloroform method. The extracts were contaminated with a final concentration 200 nM 13desmethyl C spirolide or 300 nM GYM. The concentration was calculated using a four parameter fit or a logarithmic fit of the matrix extract calibration curves of 13desmethyl C spirolide and GYM, respectively (mean ± SD, n = 4). 13-desmethyl C spirolide (%) Gymnodimine (%) Mussel Cockle Clam Scallop 87.4 ± 5.3 91.5 ± 7.5 86.9 ± 7.7 92.8 ± 7.6 62.0± 2.8 97.8 ± 19.2 115.3 ± 12.9 85.5 ± 14.3 Table 4. Recovery rate of 13-desmethyl C spirolide and GYM in shellfish extracted with the acetone/chloroform method. Mussels, cockles, clams and scallops were extracted using the acetone/chloroform method. One g of homogenized meat was spiked with an amount of GYM or 13-desmethyl C spirolide equivalent to a final concentration in the extract of 300 nM or 200 nM, respectively. Noncontaminated extracts were also spiked after extraction with the same concentration of toxin. The concentration of 13-desmethyl C spirolide and GYM was calculated using a four parameter fit or logarithmic fit of the calibration curve respectively. The toxin recovery was calculated as the percent fraction of toxin in postextractioncontaminated samples that were recovered in the pre-extraction-contaminated samples (mean ± SEM, n = 4).
13-desmethyl C spirolide (nM) Gymnodimine (nM) Mussel Cockle Clam Scallop 75 Publicaciones 183.4 ± 13.0 147.3 ± 10.7 168.3 ± 23.2 147.6 ± 17.8 214.3 ± 36.5 262.3 ± 43.7 386.9 ± 94.3 300.3 ± 114.8 Table 5. Pre-extraction contamination concentrations calculated of 13desmethyl C spirolide and GYM using calibration curves in shellfish extracts. Mussels, cockles, clams and scallops were extracted using acetone/chloroform method. One g of homogenized meat was spiked with an amount of GYM or 13desmethyl C spirolide equivalent to a final concentration in the extract of 300 nM or 200 nM respectively. The concentration of toxin in the extract was calculated using a four parameter fit and logarithmic fit of the matrix extract calibration curves of 13desmethyl C spirolide and GYM, respectively (mean ± SD, n = 4).
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UNIVERSIDAD DE SANTIAGO DE COMPOSTE
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Natalia Vilariño del Río, investi
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AGRADECIMIENTOS Quisiera agradecer
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ABREVIATURAS ADN Ácido desoxirribo
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ÍNDICE
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INTRODUCCIÓN
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2 Introducción ratón. Este métod
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Carbamadas Sulfocarmadas Decarbamad
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Figura 2. Estructura química gener
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Figura 3. Estructura química del
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Figura 4. Estructura química de la
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12 Introducción Gymnodinium sellif
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14 Introducción Existe la hipótes
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Espiro-prorocentrimina 16 Introducc
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18 Introducción dos espines de amb
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Aplicaciones 20 Introducción Los e
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22 Introducción Por encima de un c
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24 Introducción Figura 15. Detecci
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26 Introducción En el campo de la
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28 Introducción y los 100 nl. La I
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2. OBJETIVOS 29 Objetivos Las ficot
Page 53 and 54: 3. PUBLICACIONES 31 Publicaciones E
Page 55: 33 Publicaciones bungarotoxina marc
Page 59 and 60: 35 Publicaciones
Page 69: Artículo 2. Feasibility of using a
Page 87: Artículo 4. Feasibility of gymnodi
Page 90 and 91: Abstract 60 Publicaciones The detec
Page 92 and 93: 62 Publicaciones problems derived f
Page 94 and 95: 64 Publicaciones added to 40 µl of
Page 96 and 97: 66 Publicaciones range was between
Page 98 and 99: 68 Publicaciones The IC50 values ob
Page 100 and 101: 70 Publicaciones [5] E. Takahashi,
Page 102 and 103: 72 Publicaciones Figure 2. 13-desme
Page 109 and 110: 4. DISCUSIÓN GENERAL 77 Discusión
Page 111 and 112: 79 Discusión general cruzada con l
Page 113: CONCLUSIONES
Page 119: BIBLIOGRAFÍA
Page 122 and 123: 84 Bibliografía amending Regulatio
Page 124 and 125: 86 Bibliografía [28] Llewellyn, L.
Page 126 and 127: 88 Bibliografía [50] Khan, D., Ahm
Page 128 and 129: 90 Bibliografía [69] Garthwaite, I
Page 130 and 131: 92 Bibliografía [88] Takai, A., Mu
Page 132 and 133: 94 Bibliografía [108] Leira, F., A
Page 134 and 135: 96 Bibliografía [128] Kharrat, R.,
Page 136 and 137: 98 Bibliografía [146] Takada, N.,
Page 138 and 139: 100 Bibliografía [169] Nasir, M. S
Page 140: RESUMEN Las ficotoxinas marinas son
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TESIS FICOTOXINAS MARINAS EVA FONFRIA

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