TESIS FICOTOXINAS MARINAS EVA FONFRIA

13-desmethyl C spirolide
(nM)
Gymnodimine
(nM)
Mussel Cockle Clam Scallop
74
Publicaciones
213.4 ± 21.2 163.6 ± 22.2 194.8 ± 12.7 160.2 ± 14.1
341.0 ± 73.3 294.4 ± 94.3 339.9 ± 74.0 359.2 ± 92.4
Table 3. Post-extraction contamination concentrations calculated of 13desmethyl
C spirolide and GYM using calibration curves in shellfish extracts.
Mussels, cockles, clams and scallops were extracted using the acetone/chloroform
method. The extracts were contaminated with a final concentration 200 nM 13desmethyl
C spirolide or 300 nM GYM. The concentration was calculated using a
four parameter fit or a logarithmic fit of the matrix extract calibration curves of 13desmethyl
C spirolide and GYM, respectively (mean ± SD, n = 4).
13-desmethyl C spirolide
(%)
Gymnodimine
(%)
Mussel Cockle Clam Scallop
87.4 ± 5.3 91.5 ± 7.5 86.9 ± 7.7 92.8 ± 7.6
62.0± 2.8 97.8 ± 19.2 115.3 ± 12.9 85.5 ± 14.3
Table 4. Recovery rate of 13-desmethyl C spirolide and GYM in shellfish
extracted with the acetone/chloroform method. Mussels, cockles, clams and
scallops were extracted using the acetone/chloroform method. One g of homogenized
meat was spiked with an amount of GYM or 13-desmethyl C spirolide equivalent to
a final concentration in the extract of 300 nM or 200 nM, respectively. Noncontaminated
extracts were also spiked after extraction with the same concentration
of toxin. The concentration of 13-desmethyl C spirolide and GYM was calculated
using a four parameter fit or logarithmic fit of the calibration curve respectively. The
toxin recovery was calculated as the percent fraction of toxin in postextractioncontaminated
samples that were recovered in the pre-extraction-contaminated
samples (mean ± SEM, n = 4).