Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-126
Table AX5-8.2. Regulation of Bone Cell Function in Animals—Systemic Effects of Lead
Compound
Dose/Concentration
Duration Exposure
Route Species Effects Blood Level Reference
Pb acetate
30 mg/kg
Single i.v. injection
Pb acetate
0.82%
1 wk
In diet
Pb acetate
0.15% or 0.45%
GD 4 until Day 55
In drinking water
Rat Groups of male rats were killed 0.5, 5, 15, and 30 min and 1, 2, 6, and 12 h after the single
Pb injection. Serum calcium and phosphorus levels both initially increased after Pb
injection with serum phosphorus reaching a maximum value (13.5 mg%) after 30 min and
calcium (17 mg%) after 1 h. Calcium and phosphorus levels decreased after 1 h and
returned to baseline levels after 12 h.
Rat
Ingestion of 0.82% Pb in male rats fed either a low phosphorus or low calcium diet reduced
plasma levels of 1,25-(OH) 2CC, while Pb had no effect in rats fed either a high calcium diet
or a normal phosphorus diet.
Effect of Pb on serum 1,25-(OH)2CC levels in rats fed low P or normal P diet
Dietary Phosphorus
0.1%
0.1%
0.1%
0.3%
0.3%
0.3%
Supplement
Control
Cholecalciferol
0.82% Pb+Cholecalciferol
Control
Cholecalciferol
0.82% Pb+Cholecalciferol
Serum 1,25-(OH) 2CC