Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-103
Compound
Table AX5-6.17. Genotoxic/Carcinogenic Effects of Lead—Epigenetic Effects and Mixture
Interactions—DNA Repair—Animal
Assay (Concentration and
Exposure Time)
Pb acetate Cytotoxicity (0.5–5 µM for 24 h)
Mutagenesis—HPRT (0.5–5 µM
for 44h)
SCE (1–10 µM for 26 h+)
Pb acetate Mutagenesis—gpt
(0.5–1700 mM for 24 h)
DNA strand breaks as
supercoiled relaxation
(1000 mM for 24 h)
Abbreviations
G12–CHV79 are derived from V79;
V79 are a Chinese Hamster Lung Cell Line;
Medium and Components
AMEM—Alpha Minimal Essential Medium;
FBS—Fetal Bovine Serum
Cell Type
and Culture
Medium Co-exposure Effects Reference
V79 in
AMEM + 10%
FBS
G12–CHV79
cells with 1
copy gpt gene
in Ham’s F12
+ 5% FBS
UV (5 J/m 2 ) Pb acetate ( 3 and 5 µM) increased UV-induced increased cytotoxicity
with no dose response (plateau). There were no statistical analyses of
these data.
Pb acetate (0.5–5 µM) increased UV mutagenicity though with no
dose response (plateau). There were no statistical analyses of these
data
Pb acetate (1–10 µM) increased UV-induced SCE. Significant at
p < 0.01. Only 25 cells per treatment analyzed.
UV (2 J/m 2 ), or MNNG
(0.5 µg/L)
Pb acetate was co-mutagenic with UV and MNNG increasing
frequency 2-fold.
Pb acetate does not increase strand breaks induced by UV.
Hartwig et al. (1990)
Roy and Rossman
(1992)