Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-100
Table AX5-6.14. Genotoxic/Carcinogenic Effects of Lead—Epigenetic Effects and Mixture Interactions—Animal
Compound Exposure Regimen Species Co-exposure Effects Reference
Pb acetate Administered as an i.p. injection of
100 µl/kg.
Animals were studied either 24 h
after a daily dose for 3 days or for
various times (5 min–48 h) after a
single dose.
Pb nitrate Administered as an i.p. injection of
100 µmol/kg.
Animals were studied either 24 h
after a daily dose for 3 days or for
various times (5 min–48 h) after a
single dose.
Pb nitrate Administered as an i.p. injection of
100 µmol/kg. Some rats were
partially hepatectomized.
Animals were studied 48 h after
injection.
Pb nitrate Administered as an i.v. injection of
20, 50, or 100 µmol/kg.
Animals were studied 24 h after
injection.
Male Wistar Rats—
10 wks old
Male Wistar Rats—
10 wks old
Male Sprague
Dawley rats
Male Fisher 344
rats—7 wks old
Actinomycin D
(0.8 mg/kg)
administered i.p. for
4 h before a single
dose of Pb acetate.
Actinomycin D
(0.8 mg/kg)
administered i.p. for
4 h before a single
dose of Pb acetate.
Pb acetate induced GST-P, which required the cis element, GPEI
(GST-P enhancer I).
Actinomycin D blocked the effects indicating that regulation was
at the mRNA level.
Pb acetate induced c-jun, which exhibited three peaks of exposure
over 48 h.
Pb acetate was more potent than Pb nitrate.
Pb nitrate induced GST-P, which required the cis element, GPEI
(GST-P enhancer I).
Actinomycin D blocked the effects indicating that regulation was
the mRNA level.
Pb nitrate induced c-jun, which exhibited three peaks of exposure
over 48 h.
Pb nitrate was less potent than Pb acetate.
Partial Hepatectomy Pb nitrate induced GSH and GST 7-7 activity.
Partial hepatectomy did not induce GSH or GST 7-7.
2-methoxy-
4-aminobenzene to
induce P4501A2
or
3-methylcholanthrene
to induce 4501A1
Pb nitrate selectively inhibited P4501A2 and its induction by 2methoxy-4-aminobenzene
at the mRNA and protein level in a
dose-dependent manner.
Pb nitrate had minimal effect on P4501A1 and its induction by 3-
methyl cholanthrene.
Pb nitrate did not affect microsomal activity.
Pb nitrate induced GST-P in a dose-dependent manner.
Suzuki et al.
(1996)
Suzuki et al.
(1996)
Dock (1989)
Degawa et al.
(1993)