Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-80
Table AX5-6.3 (cont’d). Genotoxic/Carcinogenic Effects of Lead—Carcinogenesis Animal Cell Cultures
Compound Dose and Duration Cell Type Co-exposure Effects Reference
Pb nitrate Morphological Transformation
(0.04–8 µg/mL as Cr for 7 days)
Abbreviations
Primary SHE cells in
DMEM + 10% FCS
Cells
SHE—Syrian hamster embryo;
C3H10T/12 cells are a mouse embryo cell line
Medium and Components
AMEM—Alpha Minimal Essential Medium;
DMEM—Dulbecco’s Minimal Essential Medium;
EMEM—Eagle’s Minimal Essential Medium;
FBS—Fetal Bovine Serum
FCS—Fetal Calf Serum
H-MEM—Minimum essential medium/nutrient mixture–F12-Ham
HOS TE—Human osteosarcoma cell line TE
Differences between the serum are unclear as insufficient details are provided by authors to distinguish.
Calcium chromate Pb nitrate alone did not induce significant levels of
transformation.
Pb nitrate plus calcium chromate increased the potency of
calcium chromate to that of Pb chromate. Data suggest Pb
ions are synergistic with chromate ions in inducing
neoplastic transformation.
Elias et al. (1991)