Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-73
Reference
Fujiwara and
Kaji
(1999)
Kaji et al.
(1992)
Table AX5-5.6 (cont’d). Effects of Lead on Cultured Endothelial Cell Proliferation, Angiogenesis, and
Production of Heparan Sulfate Proteoglycans and tPA
Species/
Tissue
Bovine aorta
endothelial
cells (growing
10% BCS)
Human
umbilical vein
endothelial
cells
(confluent)
Pb Exposure Measured Parameters
Age/
Weight n Dosage Duration Pb Level CVS Other Interventions Results
— 4 sets per
experiment
— 5 sets per
experiment
Pb nitrate
0.1 µM
48 hrs Sulfate and
glucosamine
incorporation in GAGs,
quantification of high
and low MW-HSPG,
identification of
perlecan core protein
0.01–1 µM t-PA release, DNA
synth, protein synth
(leucine incorporation)
Thrombin and
ET-1
stimulations
In growing cells, Pb
depressed high-MW
HSPGs production but
had little effect on low-
MW HSPGx (~50 KD).
The core protein of
perlecan (400 KD) was
significantly reduced by
Pb exposure.
Pb exposure reduced
basal and thrombinstimulation
t-PA release
and worsened ET-1
induced inhibition of t-
PA release.