Air Quality Criteria for Lead Volume II of II - (NEPIS)(EPA) - US ...

AX5-71
Reference
Kaji et al.
(1995a)
Kaji et al.
(1995b)
Fujiwara et al.
(1998)
Kishimoto
et al. (1995)
Ueda D. et al.
(1997)
Species/
Tissue
Bovine aorta
endothelial
cells
Bovine aorta
endothelial
cells
Humanumbilical
vein
endothelia
cells
Human
umbilical vein
endothelial
cells
Table AX5-5.6. Effects of Lead on Cultured Endothelial Cell Proliferation, Angiogenesis, and
Production of Heparan Sulfate Proteoglycans and tPA
Pb Exposure Measured Parameters
Age/
Weight n Dosage Duration Pb Level CVS Other Interventions Results
— 4 sets per
experiment
— 4–5 sets per
experiment
— 6 set per
experiment
— 3 sets per
experiment
— 3 sets per
experiment
Pb nitrate
5–50 µM
Pb nitrate
0.5–5 µM
Pb nitrate
5 and 10 µM
Pb acetate
1–100 FM
Pb acetate
1–100 µM
24 hrs — Endothelial
damage
24 hrs — 3H-thymidine
incorporation, cell
count,
morphology,
LDH release
48 hrs — Appearance of
cells in denuded
areas of
monolayer, DNA
synth
24 hrs — Formation of
tube-like
structures (angiogenesis
assay, on
Matrigel (BM)
24 hrs — Tube formation
on Matrigel
matrix
Co-incubation
with cadmium
Stimulation
w/βFGF and
αFGF
Stimulation
w/Zn
Addition of Pb alone
resulted in mild
deendothelialization of
the monolayers and
markedly increased
cadmium-associated
endothelial damage.
Incubation w/Pb resulted
in a concentrationdependent
reduction of
DNA synthesis and cell
count, caused some shape
change (polygonal
→spindle) and reduced
βFGF- and αFGFmediated
proliferation.
Pb inhibited appearance
of endothelial cells in the
denuded section of
monolayer and attenuated
the healing response to
Zinc.
— Pb inhibited tube
formation concentrationdependently
and tube
lengthening time
dependently.
PKC activator
and inhibitor
Pb inhibited tube
formation concentrationdependently
and tube
lengthening time
dependently. These
effects were independent
of PKC.