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Environmental Health Criteria 214

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HUMAN EXPOSURE ASSESSMENT<br />

high-volume filters 150-2000 litre/min, hours fro<br />

Viable, non-volumetric<br />

- settlement plates undefined, hours sem<br />

lar<br />

Viable, volumetric<br />

- multiple hole impactors<br />

Andersen 6-stage sampler 28.3 litre/min, 1-30 min cut<br />

Andersen 2-stage sampler 28.3 litre/min, 1-30 min cut<br />

Andersen 1-stage (N6) 28.3 litre/min, 1-30 min cut<br />

Surface Air System sampler 90/180 litre/min, 20 sec-6 min cut<br />

Eight-stage personal impactor 2 litre/min, 5-30 min cut<br />

Burkard portable sampler 10/20 litre/min, 1-9 min cut<br />

- centrifugal impactors<br />

Reuter Centrifugal sampler (RCS) ca. 40 litre/min, 20 sec-8 min cut<br />

Reuter Centrifugal Plus (RCS-Plus) ca. 50 litre/min, 30 sec-8 min cut<br />

Table 32. (continued)<br />

Method with examples Sampling rate and time Rem<br />

- rotating slit-to-agar impactors<br />

Mattson-Garvin air sampler 28 litre/min, 5-60 min cut<br />

- liquid impingers<br />

single-stage all glass impingers 12.5 litre/min cut<br />

three-stage impingers 20 litre/min cut<br />

a For detailed information see ACGIH (1995).<br />

total number of fungal particles (Horner et al., 1994). Thus, in order<br />

to optimize the information available from air sampling, both types of<br />

particle should be sampled. However, even using the best available<br />

method, a large number of airborne spores will not grow in culture and<br />

cannot be visually identified with available methods.<br />

At present, there is no standardized method for the sampling of<br />

airborne fungi, although the American Conference of Governmental<br />

Industrial Hygienists (ACGIH, 1989) and the European Commission (EC,<br />

1993) have given recommendations. An outline for selecting a<br />

bioaerosol sampler is presented by the American Conference of<br />

Governmental Industrial Hygienists (ACGIH, 1995). Selection criteria<br />

include sampling location, form of recovered particles (intact or<br />

dispersed), the need for size separation and the expected<br />

concentrations of the particles.<br />

9.4.2 Settled dust for fungi<br />

Settled house dust can be sampled for viable fungi in exactly the<br />

same way as for house dust mites and their allergens (see section<br />

9.2.2). The dust samples can be stored at room temperature but the<br />

analysis should be performed within a few days.<br />

http://www.inchem.org/documents/ehc/ehc/ehc<strong>214</strong>.htm<br />

Page 161 of 284<br />

6/1/2007

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