New Modes of GPCR Signalling
New Modes of GPCR Signalling
New Modes of GPCR Signalling
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Effects <strong>of</strong> Cscl on Synaptic Transmission at Perforant Pathway (PP)<br />
Fibers - CA3 Region <strong>of</strong> Hippocampus in Rat<br />
Min Zheng1, 2 ,Xulin Xu1, Lian-Jun Guo1*,<br />
1Department <strong>of</strong> Pharmacology, Tongji Medical College, Huazhong University <strong>of</strong> Science &<br />
Technology, 13 Hongkong Road, Wuhan 430030, China.<br />
2 Department <strong>of</strong> Pharmacology,Xianning College, 88 Xianning Thoroughfare, Xianning<br />
437100, China.<br />
Aim: To investigate the contribution <strong>of</strong> a HCN channel nonspecific blocker CsCl to synaptic<br />
transmission at PP-CA3 pathway in rat hippocampus. Methods: The population spike (PS) <strong>of</strong><br />
PP-CA3 region in rats was recorded by using the extracellular recording techniques in vivo.<br />
Tetanic stimulation(TS; consisted <strong>of</strong> 50 trains at 0.5 Hz each composed <strong>of</strong> 4 pulses at 500Hz)<br />
was administrated to observe the induction and maintenance <strong>of</strong> long-term potentiation(LTP). We<br />
observed the effects <strong>of</strong> CsCl on the induction and sustain <strong>of</strong> LTP in PP-CA3 pathway.<br />
Simultaneously, the synapse ultrastructural at hippocampal CA3 region was observed by<br />
transmission electron microscope. Results: ①The PS amplitude level persistently stabilized at<br />
281.8 ± 6.6% <strong>of</strong> baseline in 90min after giving tetanic stimulation <strong>of</strong> PP fibers in LTP-induction<br />
group and significantly higher than that in control group(97.4 ±1.8%)(n=10, P < 0.01); ②<br />
Local administration <strong>of</strong> CsCl 5µmol /L before TS, the relative PS amplitudes in each time point<br />
were all significantly lower than that in LTP-induction group (n=10, P
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