HPLC Accessories - KNAUER Advanced Scientific Instruments
HPLC Accessories - KNAUER Advanced Scientific Instruments
HPLC Accessories - KNAUER Advanced Scientific Instruments
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<strong>HPLC</strong> · SMB · OSMOMETRY<br />
Catalog<br />
Separating Techniques 2001<br />
<strong>HPLC</strong> / SMB<br />
Systems, <strong>Instruments</strong>, Software,<br />
<strong>Accessories</strong>, and Spare Parts.<br />
DIN<br />
EN ISO<br />
9001<br />
• C E R T I F I E D •<br />
Q U A L I T Y M A N A G E M E N T
Preface<br />
Dear Customer,<br />
<strong>KNAUER</strong> has been developing and distributing osmometers and <strong>HPLC</strong> instruments<br />
for nearly 40 years. During this 40 year experience we have continuously sought to<br />
maintain the highest level of quality in all of the instruments and services that we offer.<br />
We would also like to acknowledge the fact that our success in the past four decades<br />
would not have been possible without you, our customer. Your input as to new ideas<br />
and improvement has been a major factor in our advancement as a company.<br />
Especially today, as market conditions and demands change faster than ever, we<br />
rely on your feedback and we further commit ourselves to strive to provide you with<br />
reliable and market oriented products. We do this all within the framework of the<br />
demanding requirements of DIN EN ISO 9001, our quality management system.<br />
We would like to offer you help to an easy start as well as a comprehensive overview<br />
in our extended new and improved product program catalogue, Separating<br />
Techniques 2001 – <strong>HPLC</strong> / SMB. In particular, we would like to introduce both the<br />
new UNEXAS System for online sample preparation with linked chromatographic<br />
analysis and the new Amino Acid Analyzer A100 which have broadened our product<br />
range of <strong>HPLC</strong> system solutions. The new version of the <strong>HPLC</strong> Software, ChromGate ®,<br />
ensures that our system software stays up-to-date.<br />
For product information, a quotation, or order feel free to contact us at:<br />
+49 (0)30 8 09 72 70.<br />
We would like to thank you for your continuing confidence and are already looking<br />
forward to working with you in the future. We hope to see you soon at one of our<br />
<strong>KNAUER</strong> symposiums or at one of the many exhibitions in which we participate.<br />
Best regards,<br />
Alexandra Knauer Bernward Rittgerodt<br />
Managing Director and Owner Managing Director<br />
The production of this catalog<br />
was financially supported<br />
by the European Union.<br />
1
Extended Products and Services<br />
2<br />
Osmometers<br />
<strong>HPLC</strong> Columns<br />
Electronic Newsletter<br />
� Vapor Pressure Osmometer<br />
For molecular weight determination of up to 40,000 g/mol<br />
� Membrane Osmometer<br />
For molecular weight determination between 10,000 and 1,000,000 g/mol<br />
� Semimicro Osmometer<br />
For determining the total osmality of physiological fluids<br />
<strong>KNAUER</strong> has been developing and distributing osmometers for medicine, chemistry,<br />
polymer chemistry, and clinical diagnostics with great success for nearly 40 years.<br />
Feel free to request further detailed product information to be sent to you without<br />
obligation.<br />
Vapor Pressure Osmometer K-7000<br />
<strong>KNAUER</strong> offers an extensive range of columns and phases for <strong>HPLC</strong>. Our 25 years of<br />
experience in the field of chromatographical separation techniques has been invested<br />
into each and every delivered column. High quality and performance sorbents such<br />
as our Eurospher provide for perfect analysis. Every step in the manufacturing process<br />
of separation columns is optimized. Strict criteria guarantee outstanding quality in<br />
batch-to-batch reproduction.<br />
Request a copy of our »Columns and Phases for <strong>HPLC</strong>« catalog today.<br />
The <strong>KNAUER</strong> report is our company newsletter written two to three times a year.<br />
It keeps our customers informed about the newest products in our development<br />
department, applications, events and exhibitions, and advancement and changes<br />
within our company.<br />
There is a reply card attached to each report making it simple and time efficient for<br />
you to request further information regarding any of the articles.<br />
Staying »up-to-date« with a company that rapidly advances is important in today’s<br />
technology world! Therefore, don’t wait to get your copy of our report second hand!<br />
Subscribe free of charge today per E-mail, fax, or by giving us a call!
Table of Contents<br />
<strong>HPLC</strong>-Systems, <strong>Instruments</strong>, Software, <strong>Accessories</strong>, and Spare Parts<br />
Page<br />
<strong>HPLC</strong> System Solutions System proposal »Helios« – Premium <strong>HPLC</strong> analysis with maximum flexibility ....... 5<br />
System proposal »Asteris« – Isocratic stand-alone system ..................................... 6<br />
System proposal »Sacharis« – Automated sugar analysis ...................................... 7<br />
System proposal »Metis« – Optimized for semi-preparative purification ................. 8<br />
System proposal »Demeter« – Versatile semi-preparative and analytical <strong>HPLC</strong> ........ 9<br />
System proposal »Anemos« – Ultimate modular system for preparative <strong>HPLC</strong> ........ 10<br />
System proposal »Herakles« – Compact high-performance preparative system ...... 11<br />
System proposal »Zefiros« – Our exclusive explosion proof preparative system ...... 12<br />
CHANCE – The smallest <strong>HPLC</strong> system in the world .............................................. 13<br />
<strong>KNAUER</strong> Software ChromGate ® – GLP compliant instrument and data management ....................... 14<br />
Online SPE and <strong>HPLC</strong> UNEXAS – Universal Extraction and Sample Preparation ...................................... 15<br />
Simulated Moving Bed<br />
Chromatography (SMB) SMB production facility CSEP ® C912 .................................................................... 16<br />
SMB pilot system CSEP ® C916 ............................................................................. 17<br />
Amino Acid Analysis Amino Acid Analyzer A100 ................................................................................... 18<br />
Pumps and <strong>Accessories</strong> WellChrom <strong>HPLC</strong> Pump K-1001 ........................................................................... 19<br />
WellChrom <strong>HPLC</strong> Pump K-501 ............................................................................. 20<br />
WellChrom <strong>HPLC</strong> Pump K-120 ............................................................................. 21<br />
<strong>Accessories</strong> and Spare Parts for <strong>HPLC</strong> Pumps<br />
K-100, K-120, K-500, K-501, K-1000, and K-1001 ............................................... 22<br />
WellChrom Preparative <strong>HPLC</strong> Pump K-1800 ......................................................... 23<br />
WellChrom Pneumatic Pump K-1900 ................................................................... 24<br />
Preparative Pump Heads and <strong>Accessories</strong> ............................................................. 25<br />
WellChrom Solvent-Organizer K-1500 .................................................................. 26<br />
Detectors and <strong>Accessories</strong> WellChrom Diode Array Detector K-2700 ............................................................. 27<br />
WellChrom Fast Scanning Spectro-Photometer K-2600 ........................................ 28<br />
WellChrom Spectro-Photometer K-2501 .............................................................. 29<br />
WellChrom Filter-Photometer K-2001 ................................................................... 30<br />
WellChrom Spectro-Photometers K-2001, K-2501, K-2600<br />
with fiber optics ................................................................................................... 31<br />
Flow Cells, <strong>Accessories</strong>, and Spare Parts ............................................................... 32<br />
Flow Cells, Spare Parts, <strong>Accessories</strong> for Photometric Detectors ............................. 34<br />
WellChrom Differential Refractometers K-2301 and K-2401 ................................. 35<br />
Fluorescence <strong>HPLC</strong> Monitor RF-10 AXL ................................................................ 36<br />
Conductivity Detector for Ion Chromatography ................................................... 37<br />
Highly Sensitive Amperometric Detector for Clinical Diagnostics .......................... 38<br />
Light Scattering Detector ELSD 2000 ................................................................... 39<br />
3
Table of Contents<br />
<strong>HPLC</strong>-Systems, <strong>Instruments</strong>, Software, <strong>Accessories</strong>, and Spare Parts<br />
Page<br />
<strong>HPLC</strong> Columns<br />
and Electric Valve Drives WellChrom Electric Valve Drives K-6 and K-16 ...................................................... 40<br />
Injection and Switching Valves for K-6 and K-16 .................................................. 41<br />
4<br />
Automated <strong>HPLC</strong> Injection Systems Autosampler WellChrom Basic Marathon K-3800 ................................................. 42<br />
Autosampler TRIATHLON ..................................................................................... 43<br />
Autosampler ENDURANCE ................................................................................... 44<br />
Autosampler MIDAS ............................................................................................. 44<br />
Fraction Collectors Fraction Collector Retreiver 500 ........................................................................... 45<br />
Fraction Collector Foxy Jr. .................................................................................... 45<br />
Fraction Collector B-684 ...................................................................................... 45<br />
Online Degasser WellChrom Online Degasser K-5002 .................................................................... 46<br />
WellChrom Online Degasser K-5004 .................................................................... 46<br />
WellChrom Online Degasser K-5020 .................................................................... 46<br />
<strong>HPLC</strong> Mixing Chambers Dynamic Mixing Chamber, analytical version ....................................................... 47<br />
Dynamic Mixing Chamber, preparative version .................................................... 47<br />
Thermostats and Flowmeter Flowmeter ............................................................................................................ 48<br />
Column Box ......................................................................................................... 48<br />
Column Thermostat Jetstream .............................................................................. 48<br />
<strong>HPLC</strong> <strong>Accessories</strong> <strong>HPLC</strong> Standard Accessory Kit ............................................................................... 49<br />
Start-Up Kit, analytical .......................................................................................... 50<br />
Start-Up Kit, biocompatible .................................................................................. 50<br />
Start-Up Kit, preparative ....................................................................................... 51<br />
Filters for Sample Preparation ............................................................................... 51<br />
Sample Loops, analytical ...................................................................................... 52<br />
Sample Loops, preparative ................................................................................... 52<br />
Injection Syringes ................................................................................................. 52<br />
Capillaries, Stainless Steel ..................................................................................... 53<br />
Capillaries, PEEK ................................................................................................... 53<br />
Tubings, Tefzel ..................................................................................................... 54<br />
Tubings, PTFE ....................................................................................................... 54<br />
Tube Cutter, Ferrules, and Sealing Rings ............................................................... 54<br />
T and Cross Connectors, Stainless Steel, and Polymer .......................................... 55<br />
Bushings and Blind Fittings (Caps) ....................................................................... 56<br />
DYNASEAL Connection System ............................................................................ 56<br />
Column Inlet Filters .............................................................................................. 58<br />
Syringe Filtration Units ......................................................................................... 58<br />
Terms of Delivery Terms of Delivery, address, bank information ....................................................... 59<br />
Conditions of Sale Conditions of Sale ................................................................................................ 60
<strong>HPLC</strong> System Solutions<br />
System Proposal »Helios«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A41301 WellChrom <strong>HPLC</strong> Pump K-1001, includes 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A4012 WellChrom Solvent Organizer K-1500, stainless steel 1<br />
A0285 Dynamic Mixing Chamber 1<br />
A2015 WellChrom Online Degasser K-5004, 4 channels 1<br />
A4070 WellChrom Fast Scanning Spectro-Photometer K-2600 1<br />
A4061 Analytical Flow Cell, 10 mm 1<br />
A0759 WellChrom Autosampler Basic Marathon K-3800 1<br />
A1456 ChromGate ® Chromatography Data Acquisition and Management 1<br />
A1459 Scan Option for Spectro-Photometer K-2600 1<br />
A0910 Start-Up Kit, analytical 1<br />
I6Y535 VERTEX Column, Eurospher 100 C18, 5 µm, 250 x 4 mm<br />
with an integrated precolumn 1<br />
Description<br />
Premium <strong>HPLC</strong> analysis with<br />
maximum flexibility<br />
This system will satisfy your needs in<br />
all aspects of laboratory work. Whether<br />
it be the acquisition of spectras or the<br />
evaluation of peak purity, complete and<br />
GLP compliant software control is what<br />
you are looking for.<br />
This system raises the bar in terms of<br />
performance and flexibility.<br />
We lowered the bar, however, when it<br />
came to the price!<br />
5
<strong>HPLC</strong> System Solutions<br />
6<br />
Description<br />
Isocratic stand-alone system<br />
Complete isocratic stand-alone system<br />
with online degasser, software, columns,<br />
and accessories. Simply set it up,<br />
calibrate, and measure!<br />
The system is reliable and qualified for<br />
use in quality control and<br />
routine analysis.<br />
System Proposal »Asteris«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A42401 WellChrom <strong>HPLC</strong> Pump K-501 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A4160 WellChrom Filter-Photometer K-2001 1<br />
A4061 Analytical Flow Cell, 10 mm 1<br />
A1357 6-Port/3-Channel Injection Valve, 1/16”, manually driven 1<br />
A4018 Universal Mounting Bracket 1<br />
A2009 WellChrom Online Degasser K-5002, 2 channels 1<br />
A1245-4 EuroChrom ® 2000 Basic Edition V2.05, <strong>HPLC</strong> software 1<br />
A0910 Start-Up Kit, analytical 1<br />
I6Y535 VERTEX Column, Eurospher 100 C18, 5 µm, 250 x 4 mm,<br />
with precolumn 1
System Proposal »Sacharis«<br />
Ordering Information for Systems with Manual Injection<br />
Order No. Article Quantity<br />
A42401 WellChrom <strong>HPLC</strong> Pump K-501 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A2110 WellChrom Differential Refractometer K-2301 1<br />
A0527 Column Thermostat, 5 – 85 °C, with an integrated injection valve 1<br />
A2009 WellChrom Online Degasser K-5002, 2 channels 1<br />
A1245-4 EuroChrom ® 2000 Basic Edition V 2.05, <strong>HPLC</strong> software 1<br />
A0910 Start-Up Kit, analytical 1<br />
B196 VERTEX Column, Eurokat Ca, 300 x 8 mm 1<br />
Ordering Information for Systems with an Autosampler<br />
Order No. Article Quantity<br />
A42401 WellChrom <strong>HPLC</strong> Pump K-501 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A2110 WellChrom Differential Refractometer K-2301 1<br />
A0585 Column Thermostat, 5 – 85 °C, injection valve excluded 1<br />
A2009 WellChrom Online Degasser K-5002, 2 channels 1<br />
A0759 WellChrom Autosampler Basic Marathon K-3800 1<br />
A1245-4 EuroChrom ® 2000 Basic Edition V 2.05, <strong>HPLC</strong> software 1<br />
A0910 Start-Up Kit, analytical 1<br />
B196 VERTEX Column, Eurokat Ca, 300 x 8 mm 1<br />
Description<br />
Automated sugar analysis<br />
»Sacharis« can be used with or without<br />
an autosampler! With this system, you’ll<br />
have your sugar analysis under control.<br />
The proven refractive index detector<br />
and the column thermostat completes<br />
the range of this powerful system.<br />
7
<strong>HPLC</strong> System Solutions<br />
8<br />
Description<br />
Optimized for semi-preparative<br />
purification<br />
This system enables optimization and<br />
purification of substances in a semipreparative<br />
scale due to clever column<br />
switching. Prepare yourself for the<br />
majority of purification tasks in the<br />
field of semi-preparative <strong>HPLC</strong>.<br />
Its easy operation, as well as flexible<br />
adaptability guarantees the highest<br />
quality and most reliable results.<br />
System Proposal »Metis«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A42403 WellChrom <strong>HPLC</strong> Pump K-501 with semi-preparative<br />
50 ml pump head, inert with ceramic inlays 2<br />
A0285 Dynamic Mixing Chamber 1<br />
A2014 WellChrom Online Degasser K-5002, 2 channels 1<br />
A0377 WellChrom Electric Valve Drive K-6 2<br />
A1373 7-Port/1-Channel Switching Valve, 1/16”, stainless steel 2<br />
A4180 WellChrom Spectro-Photometer K-2501 1<br />
A4062 Analytical Flow Cell, 3 mm 1<br />
A0759 WellChrom Autosampler Basic Marathon K-3800 1<br />
A1170 Fraction Collector Foxy Jr. 1<br />
A1273 Diverter Valve for Fraction Collector Foxy Jr. 1<br />
A1245-4 EuroChrom ® 2000 Basic Edition V 2.05, <strong>HPLC</strong> software 1<br />
A0910 Start-Up Kit, analytical 1<br />
I6Y535 VERTEX Column, Eurospher 100 C18 5 µm, 250 x 4 mm<br />
with an integrated precolumn 1<br />
B27Y537 VERTEX Column, Eurospher 100 C18, 10 µm, 250 x 16 mm 1
System Proposal »Demeter«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A41303 WellChrom <strong>HPLC</strong> Pump K-1001 with semi-preparative<br />
50 ml pump head, inert with ceramic inlays 2<br />
A2014 WellChrom Online Degasser K-5020, 2 channels,<br />
biocompatible, preparative version 1<br />
A0275 Dynamic Mixing Chamber, Titanium 1<br />
A4018 Universal Mounting Bracket<br />
A1358 6-Port/3-Channel Injection Valve, 1/16’’, PEEK 1<br />
A1057 Sample Loop, 500 µl, 1/16’’, PEEK 1<br />
A4180 WellChrom Spectro-Photometer K-2501 1<br />
A4063 Analytical Flow Cell, 3 mm, PEEK 1<br />
A4733 EuroChrom ® for Windows, Preparative Version V 2.05 1<br />
A1170 Fraction Collector Foxy Jr. 1<br />
A1273 Diverter Valve for Fraction Collector Foxy Jr. 1<br />
A1039 Start-Up Kit, biocompatible 1<br />
Order No. Optional instead of A41303 Quantity<br />
A42403 WellChrom <strong>HPLC</strong> Pump K-501 with semi-preparative<br />
50 ml pump head, inert with ceramic inlays 2<br />
Description<br />
Versatile semi-preparative and<br />
analytical <strong>HPLC</strong><br />
This system helps the transition from<br />
analytical to semi-preparative biochromatography.<br />
Outstanding performance<br />
together with an outstanding price.<br />
Customized columns are available<br />
upon request.<br />
9
<strong>HPLC</strong> System Solutions<br />
Description<br />
Ultimate modular system for<br />
preparative <strong>HPLC</strong><br />
Everything is included even for the<br />
most precise preparative <strong>HPLC</strong> applications.<br />
<strong>KNAUER</strong> exclusively offers<br />
a complete high pressure gradient<br />
package rated up to a maximum of<br />
200 bar in perfect harmony.<br />
Its minimal use of space is outstanding!<br />
10<br />
System Proposal »Anemos«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A40201 WellChrom Preparative Pump K-1800 with<br />
preparative 250 ml pump head 2<br />
A0581 Dynamic Mixing Chamber, preparative version 1<br />
A2515 Mixing Chamber Extension Unit, 4.9 ml 1<br />
A41501 WellChrom <strong>HPLC</strong> Pump K-120 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A0377 WellChrom Electric Valve Drive K-6 1<br />
A1371 6-Port/3-Channel Switching Valve, 1/8’’, stainless steel 1<br />
A1375 7-Port/1-Channel Switching Valve, 1/8’’, stainless steel 1<br />
A1160 Preparative Sample Loop, 1/8’’, stainless steel 1<br />
A4180 WellChrom Spectro-Photometer K-2501 1<br />
A4066 Preparative Flow Cell, 1/8’’, stainless steel,<br />
variable layer thickness 0.5 mm to 2.0 mm 1<br />
A4733 EuroChrom ® for Windows, Preparative Version V 2.05, <strong>HPLC</strong> Software 1<br />
A1104 <strong>HPLC</strong> Start-Up Kit, preparative 1<br />
B31Y536 VERTEX Column, Eurospher 100 C18, 7 µm, 250 x 32 mm 1
System Proposal »Herakles«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A40201 WellChrom Preparative Pump K-1800 with<br />
250 ml pump head 1<br />
A1359 6-Port/3-Channel Injection Valve, 1/8”, stainless steel 1<br />
A1363 7-Port/1-Channel Injection Valve, 1/8”, stainless steel 1<br />
A1160 Preparative Sample Loop, 45 ml, 1/8”, stainless steel 1<br />
A4160 WellChrom Filter-Photometer K-2001 1<br />
A4066 Preparative Flow Cell, 1/8”, stainless steel,<br />
variable layer thickness 0.5 to 2.0 mm 1<br />
A0675 1-Channel Recorder 1<br />
A0790 Chart Paper for A0675 10<br />
A4018 Universal Mounting Bracket 1<br />
A1104 Start-Up Kit, preparative 1<br />
B32Y537 VERTEX Column, Eurospher 100 C18, 10 µm, 120 x 32 mm 1<br />
Description<br />
Compact high-performance<br />
preparative system<br />
Achieve pure substances for monitoring,<br />
determination of the chemical<br />
structure, or simply as an end product.<br />
This compact and powerful system can<br />
be placed just about anywhere! Even<br />
on your shopping list!<br />
11
<strong>HPLC</strong> System Solutions<br />
Description<br />
Our exclusive explosion proof<br />
preparative system<br />
Our exclusive fiber optics enable the<br />
separation of the measuring cell from<br />
the instrument. Thus, optimal positions<br />
can be achieved for the cell due to its<br />
miniature housing for use in hazardous<br />
conditions.<br />
Hazard proof pumps are available<br />
upon request.<br />
12<br />
System Proposal »Zefiros«<br />
Ordering Information<br />
Order No. Article Quantity<br />
A40202 WellChrom Preparative Pump K-1800 with preparative<br />
1,000 ml pump head 1<br />
A1359 6-Port/3-Channel Injection Valve, 1/8”, stainless steel 1<br />
A1363 7-Port/1-Channel Injection Valve, 1/8”, stainless steel 1<br />
A1160 Preparative Sample Loop, 45 ml, 1/8”, stainless steel 1<br />
A4210 WellChrom Spectro-Photometer K-2501 with fiber optics 1<br />
A4078 Preparative Flow Cell, 1/8”, stainless steel,<br />
variable layer thickness 0.5 to 2.0 mm, with fiber optics 1<br />
A0675 1-Channel Recorder 1<br />
A0790 Chart Paper for A0675 10<br />
A4018 Universal Mounting Bracket 1<br />
A1104 Start-Up Kit, preparative 1<br />
B141Y537 VERTEX Column, Eurospher 100 C18, 10 µm , 250 x 40 mm 1
CHANCE – The smallest <strong>HPLC</strong> System in the World<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4330 CHANCE System, isocratic:<br />
WellChrom <strong>HPLC</strong> Pump K-120 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
WellChrom Fixed Wavelength Detecor K-200 1<br />
Analytical Flow Cell, 10 mm 1<br />
6-Port/3-Channel Injection Valve, 1/16’’, manually driven 1<br />
VERTEX Column, Eurospher 100 C18, 5 µm, 125 x 4 mm 1<br />
Set of accessories and spare parts 1<br />
A4440 CHANCE-Software for Windows 95/98, NT 1<br />
A4450 HPG Extension Kit:<br />
WellChrom <strong>HPLC</strong> Pump K-120 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
Static Mixing Chamber 1<br />
Set of accessories 1<br />
A4460 CHANCE System, semi-preparative:<br />
50 ml pump head, inert with ceramic inlays 1<br />
Semi-preparative flow cell, 3 mm 1<br />
Semi-preparative sample loop, 100 µl 1<br />
WellChrom Online Degasser K-5002, 2 channels 1<br />
Set of accessories 1<br />
A0676 Manometer (0 – 400 bar) with Universal Mounting Bracket for<br />
WellChrom <strong>HPLC</strong> Pump K-120 1<br />
Description<br />
This tiny new system is very practical<br />
for routine <strong>HPLC</strong> analysis with UV detection.<br />
It fits into the smallest spot in the<br />
laboratory and is very »mobile«. Even a<br />
battery can be used for energy supply.<br />
Thus, outdoor right-in-place analysis is<br />
possible. The system is suitable for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Semi-Preparative <strong>HPLC</strong><br />
The RS-232 connector enables external<br />
control of the pump and detector.<br />
Optional<br />
Powerful CHANCE-Software is for data<br />
acquisition and instrument control,<br />
quantitative peak evalution according<br />
to the methods of internal and external<br />
standard, and more sophisticated<br />
features.<br />
The CHANCE-System can be extended<br />
to a binary high pressure gradient<br />
(HPG) system, if a second WellChrom<br />
<strong>HPLC</strong> Pump K-120 and a static mixing<br />
chamber are used. Extension to a semipreparative<br />
system is also possible.<br />
Refer to page 21 for detailed information<br />
on the <strong>HPLC</strong> pump K-120.<br />
13
<strong>KNAUER</strong> Software<br />
Description<br />
ChromGate ® is a real 32-bit chromatography<br />
data system based on the<br />
newest technology from Windows 95,<br />
Windows 98, and Windows NT.<br />
ChromGate ® provides powerful and<br />
sophisticated features in one package<br />
and comes with an easy-to-use interface.<br />
The software includes creation<br />
of methods, design of custom reports,<br />
calibration functions according to<br />
proven chromatographic standard<br />
methods, data acquisition, peak processing,<br />
and running automated<br />
sample sequences.<br />
With ChromGate ® it is possible to<br />
control numerous independent chromatographic<br />
systems connected to<br />
<strong>KNAUER</strong> or third party instruments.<br />
ChromGate’s ® GLP compliant import<br />
and export functions, as well as its<br />
client/server properties, guarantee a<br />
perfect integration into your local area<br />
network or Laboratory Information<br />
Management System (LIMS).<br />
14<br />
ChromGate ® – System Control and Data Acquisition<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1456-2 ChromGate ® Data System V2.8, third release,<br />
License for 1 <strong>HPLC</strong> system 1<br />
A1457-2 Additional license for one more <strong>HPLC</strong> system 1<br />
A1458-2 Additional license to process chromatographic data on another<br />
offline computer 1<br />
A1459-2 Additional license to support <strong>KNAUER</strong>’s<br />
WellChrom Fast Scanning Spectro-Photometer K-2600 1<br />
A1460-2 Additional license to support <strong>KNAUER</strong>’s<br />
WellChrom Diode Array Detector K-2150 and K-2700 1<br />
A1461-2 Additional license to support system suitability options such as<br />
customized calculations along with a method 1<br />
A1469-2 ChromGate ® Client License 1<br />
A1470-2 ChromGate ® GPC option 1<br />
A1472 Upgrade to ChromGate ® V 2.8, third release 1<br />
A1311 Personal Computer for ChromGate ® Data System:<br />
The computer corresponds to the ChromGate ® hardware<br />
requirements. Complete functionality is guaranteed in a<br />
client/server network. 1<br />
A1312 Color Monitor, 17’’ 1<br />
A1493-2 ChromGate ® Data System V2.8, third release,<br />
Client/Server license for 1 <strong>HPLC</strong> system 1<br />
A1494-2 Additional Client/Server license for one more <strong>HPLC</strong> system 1<br />
A1495-2 Client license for Client/Server environment 1<br />
Additional Software Products<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1468 Upgrade from EuroChrom ® 2000 <strong>HPLC</strong> software to ChromGate ®<br />
Data System V 2.8, third release 1<br />
A1245-4 EuroChrom ® 2000 Basic Edition V 2.05, interface box excluded 1<br />
A4733-1 EuroChrom ® 2000 for Windows, Preparative Version V 3.01 1<br />
A1347-2 Upgrade EuroChrom ® 2000 Integration Package to V 1.65 1<br />
A13082 Interface Box 1
Online SPE and <strong>HPLC</strong><br />
UNEXAS – Online Sample Preparation<br />
Ordering Information<br />
Order No. Article Quantity<br />
A30001 UNEXAS, fully automated system for online-sample<br />
preparation in <strong>HPLC</strong> 1<br />
A0320 Autosampler TRIATHLON 1<br />
A1482 UNEXAS SPE cartridge holder 1<br />
A1483 UNEXAS SPE cartridge HySphere-Resin H 100<br />
Description<br />
UNEXAS (Universal Extraction and<br />
Sample Preparation) couples online<br />
solid phase extraction (SPE) to <strong>HPLC</strong>.<br />
UNEXAS is great for pharmaceutical<br />
chemistry, diagnostics, forensic analysis,<br />
food chemistry, and environmental<br />
analysis.<br />
UNEXAS grants an optimal sample<br />
throughput with a minimum consumption<br />
of eluent. Because this system<br />
minimizes the carry-over of samples,<br />
your results will be astonishing!<br />
The system is completely controlled<br />
by our GLP compliant <strong>HPLC</strong> software<br />
ChromGate ® which guarantees the<br />
highest degree of automation.<br />
Combine UNEXAS with the detector<br />
of your choice and you will receive a<br />
full analytical <strong>HPLC</strong> system that can<br />
process binary high pressure gradients.<br />
Control<br />
Completely controlled by ChromGate ®<br />
Power Supply<br />
115/220 V, 50/60 Hz<br />
Dimensions and Weight<br />
360 x 510 x 350 mm (W x H x D),<br />
21.0 kg<br />
Important Note<br />
For proper operation, UNEXAS requires<br />
the TRIATHLON autosampler which is<br />
excluded in the standard delivery!<br />
15
Simulated Moving Bed<br />
Chromatography (SMB)<br />
Description<br />
A System that produces up to<br />
100 kg of pure compounds per year<br />
The SMB System CSEP ® C916 is used<br />
for extracting pure substances on a<br />
semi-preparative scale. Like the »big«<br />
system, CSEP ® C912, the pilot facility<br />
enables continuous operation and<br />
high efficiency.<br />
The system operates with a thermostat<br />
and can hold up to 16 SMB columns of<br />
equal type. The patented Multi-Port<br />
Valve with 64 ports guarantees a compact<br />
design that maintains an incredibly<br />
small total dead volume.<br />
The continuous product feed is distributed<br />
across the entire surface of the stationary<br />
phase. Thus, the extraction<br />
yields maximum results.<br />
Important Note<br />
Due to individual requirements,<br />
columns are excluded from the standard<br />
delivery. We can recommend appropriate<br />
columns and packing materials<br />
according to your specific needs.<br />
Description<br />
Powerful Software for Preparative<br />
<strong>HPLC</strong> and SMB: SMB_Guide ® ,<br />
ChromSim ® , and IsothermFit ®<br />
Based on the well established algorithms<br />
of Prof. Guiochon (University of Tennessee,<br />
U.S.A.) and Prof. Dr. Seidel-Morgenstern<br />
(Max-Planck-Institute for Dynamics<br />
of Complex Technical Systems, Magdeburg,<br />
Germany), SMB and isocratic<br />
separations can be predicted and<br />
simulated on a computer.<br />
This set of powerful chromatographic<br />
software tools guarantee fast method<br />
development and perfect results.<br />
Let’s have a closer look at some of the<br />
advantages:<br />
• Estimation of eluent consumption,<br />
productivity, and product purity<br />
• Independent of vendors<br />
• No real measurements are required<br />
• Optimization for up to three compounds<br />
• Works for SMB and preparative <strong>HPLC</strong><br />
16<br />
SMB Pilot Facility CSEP ® C916<br />
Ordering Information<br />
Order No. Article Quantity<br />
A28521 SMB Pilot Facility CSEP ® C916 1<br />
Consists of:<br />
WellChrom <strong>HPLC</strong> Pump K-501 with 10 ml or 50 ml pump head,<br />
inert with ceramic inlays, stainless steel 4<br />
64-Port Multi-Function Valve, Hastelloy (stainless steel),<br />
1/16” connectors, pressure stability 80 bar, for up to<br />
16 equal SMB columns with either max. length of 300 mm<br />
or max. 20 kg of weight 1<br />
Column Thermostat (up to 80 °C) 1<br />
Rack on castors, 30 x 35 cm 1<br />
Set of capillaries, fittings, and accessories (1/16”) 1<br />
PC including pre-configured ValveChrom-Software 1<br />
Order No. Additional Software Products Quantity<br />
A2865 SMB_Guide ® for Windows<br />
Software for simulation and optimization of SMB separations 1<br />
A2866 Simulation software ChromSim ® for Windows<br />
Software for simulation of peak recycling and fraction collection 1<br />
A2867 Evaluation software IsothermFit ® for Windows<br />
Software for determining non-linear adsorption isotherms<br />
when working with high concentrations 1
SMB Production Facility CSEP ® C912<br />
Ordering Information<br />
Order No. Article Quantity<br />
A2850 SMB Production Facility CSEP ® C912 1<br />
Consists of:<br />
WellChrom Preparative Pump K-1800, 1<br />
with 100 ml, 250 ml, oder 1,000 ml pump head, stainless steel 4<br />
48-Port Multi-Function Valve, Hastelloy (stainless steel),<br />
1/8’’-connectors, 80 bar pressure stability, for up to 12 equal<br />
SMB columns with either a max. length of 1,000 mm or<br />
a max. weight of 120 kg 1<br />
Column Thermostat (up to 80 °C) 1<br />
Rack on castors 50 x 50 cm 1<br />
Set of capillaries, fittings, and accessories 1<br />
PC including pre-configured ValveChrom-Software 1<br />
Order No. Additional Software Products Quantity<br />
A2865 SMB_Guide ® for Windows<br />
Software for simulation and optimization of SMB separations 1<br />
A2866 ChromSim ® for Windows<br />
Software for simulation of peak recycling and fraction collection 1<br />
A2867 IsothermFit ® for Windows<br />
Software for determining of non-linear adsorption isotherms<br />
when working with high concentrations 1<br />
Description<br />
System for producing up to 1,000 kg<br />
of pure compounds per year!<br />
The SMB System CSEP ® C912 is known<br />
for its continous operation and high<br />
productivity. The continous delivery of<br />
feed and desorbent enables permanent<br />
extraction of two fractions at high<br />
concentrations.<br />
The thermostated system can hold up to<br />
12 SMB columns of similar type in terms<br />
of column packing, column dimensions<br />
etc. The patented Multi-Port Valve with<br />
its 48 ports is the »heart« of the system.<br />
The compact design of CSEP ® C912<br />
dramatically reduces the total dead<br />
volume compared to other available<br />
SMB concepts.<br />
The product is distributed across the<br />
entire surface of the stationary phase.<br />
Resulting in a highly efficient system.<br />
When peak recycling is practiced, one<br />
can save up to 90 % of eluent consumption!<br />
Yet this is still not sufficient;<br />
therefore, another savings of up to 80 %<br />
can be achieved with the column<br />
packing materials. You’re asking how?<br />
It’s simple. Neither is there a need for<br />
small particle sizes, nor for large plate<br />
numbers. This keeps column packing<br />
inexpensive!<br />
Software for process simulation and<br />
optimization is available.<br />
Important Note<br />
Due to individual requirements,<br />
columns are excluded from the standard<br />
delivery. We can recommend appropriate<br />
columns and packing materials<br />
according to your specific needs.<br />
17
Amino Acid Analysis<br />
Description<br />
The Amino Acid Analyzer A100<br />
(aminoSys) has been specifically<br />
developed for applying ready-to-use<br />
Ninhydrin reagent to a post-column<br />
derivatization or pre-column derivatization<br />
with OPA.<br />
The »A100« is distinguished by its<br />
capability of working with ready-to-use<br />
buffer solutions. Thus, the system<br />
analyzes 43 physiological amino acids<br />
in less than 90 minutes! At the same<br />
time, its design is as compact as its<br />
price.<br />
The Amino Acid Analyzer A100<br />
(aminoSys) processes sequences of<br />
up to 120 samples (1.5 ml vials)<br />
and uses a service free 570 nm UV<br />
detection.<br />
Delivery System<br />
Double-piston pump with an electronic<br />
residual pulsation suppressor<br />
Flow Ranges<br />
0.001 – 1.5 ml/min<br />
flow rate accuracy: < 1 % RSD<br />
(measured at 100 µl/min)<br />
Max. Operating Pressure<br />
400 bar<br />
Detection<br />
Service-free Micro-Photometer with<br />
570 nm fixed wavelength (optional<br />
440 nm), cell volume 10 µl, layer<br />
thickness 10 mm, autozero<br />
Temperature Control<br />
Peltier-element, column thermostat<br />
with adjustable temperature<br />
(20 °C – 80 °C),<br />
reactor with adjustable temperature<br />
(50 °C – 150 °C)<br />
Control<br />
Complete software control, optional<br />
control using keyboard and display<br />
18<br />
Amino Acid Analyzer A100 (aminoSys)<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0991 Amino Acid Analyzer A100 (aminoSys),<br />
complete with software 1
Pumps and <strong>Accessories</strong><br />
WellChrom <strong>HPLC</strong> Pump K-1001<br />
Ordering Information<br />
Order No. Article Quantity<br />
A41301 WellChrom <strong>HPLC</strong> Pump K-1001 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A41303 WellChrom <strong>HPLC</strong> Pump K-1001 with 50 ml pump head,<br />
inert with ceramic inlays 1<br />
A0231 Biocompatible Titanium Capillary-Kit for pump head<br />
and pressure unit for A41301 and A41303 1<br />
Refer to page 22 for more information on pump heads and accessories.<br />
Refer to page 26 for more details on the WellChrom Solvent Organizer K-1500.<br />
The <strong>HPLC</strong> pumps of the WellChrom family, in particular the K-120, K-501, and K-1001<br />
are delivered with the same mechanical drive. Thus, all pumps can be equipped with<br />
the same type of pump head. Spare parts can also be used with the K-100, K-500,<br />
and K-1000.<br />
Description<br />
Interchangeable inert pump heads for:<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Semi-preparative <strong>HPLC</strong><br />
This pump contains the logic needed<br />
for gradient operation. It can be extended<br />
without the assistence of a PC<br />
with the WellChrom Solvent Organizer<br />
K-1500 to receive a low pressure<br />
gradient system up to four solvents.<br />
Multiple pumps can be used to form a<br />
high pressure gradient with up to four<br />
solvents.<br />
Adding an electric switching valve will<br />
make a solvent recycling or fraction<br />
collection feature available.<br />
Delivery System<br />
Double-piston pump with an electronic<br />
residual pulsation suppressor<br />
Flow Ranges<br />
10ml pump head: 0.001–9.999ml/min*<br />
50ml pump head: 0.001–49.99ml/min<br />
* 3 digits with software control only!<br />
Flow Rate Accuracy<br />
RSD < 0.5 % (measured at 1 ml/min)<br />
Maximum Operating Pressure<br />
10 ml pump head: 400 bar,<br />
50 ml pump head: 150 bar<br />
System Protection<br />
Soft start with adjustable Pmin and Pmax Control<br />
2 RS-232 connectors, 8 event outputs<br />
(TTL, OC, relays), remote connection<br />
(start, error, pressure)<br />
The pump can be controlled with<br />
ChromGate ® software<br />
Analog Flow Rate Control<br />
0–10V<br />
Display<br />
2 x 24 characters<br />
Dimensions and Weight<br />
160 x 185 x 340 mm (W x H x D),<br />
5.2 kg<br />
19
Pumps and <strong>Accessories</strong><br />
Description<br />
Interchangeable inert pump heads for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Semi-preparative <strong>HPLC</strong><br />
Isocratic <strong>HPLC</strong> pump for one solvent<br />
composition. A high pressure gradient<br />
system with up to four solvents can be<br />
formed when using multiple pumps.<br />
Delivery System<br />
Double-piston pump with main and<br />
auxiliary piston<br />
Flow Ranges<br />
10ml pump head: 0.001–9.999ml/min*<br />
50ml pump head: 0.001–49.99ml/min<br />
* 3 digits with software control only!<br />
Maximum Operating Pressure<br />
10 ml pump head: 400 bar/150 bar<br />
(stainless steel or ceramic/PEEK)<br />
50 ml pump head: 150 bar<br />
System Protection<br />
Soft start with adjustable Pmin and Pmax Control<br />
RS-232 connector, remote connection<br />
(start, stop, flow)<br />
The pump can be controlled by<br />
EuroChrom ® 2000 V 1.65 or higher<br />
Display<br />
2 x 16 characters<br />
Dimensions and Weight<br />
105 x 186 x 340 mm (W x H x D),<br />
3.4 kg<br />
20<br />
WellChrom <strong>HPLC</strong> Pump K-501<br />
Ordering Information<br />
Order No. Article Quantity<br />
A42401 WellChrom <strong>HPLC</strong> Pump K-501 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A42403 WellChrom <strong>HPLC</strong> Pump K-501 with 50 ml pump head,<br />
inert with ceramic inlays 1<br />
A0248 Biocompatible Titanium Capillary-Kit for pump head<br />
and pressure unit for A42401 and A42403 1<br />
Refer to page 22 for more information on pump heads and accessories.<br />
The <strong>HPLC</strong> pumps of the WellChrom family, in particular the K-120, K-501, and K-1001<br />
are delivered with the same mechanical drive. Thus, all pumps can be equipped with<br />
the same type of pump head. Spare parts can also be used with the K-100, K-500,<br />
and K-1000.
WellChrom <strong>HPLC</strong> Pump K-120<br />
Ordering Information<br />
Order No. Article Quantity<br />
A41501 WellChrom <strong>HPLC</strong> Pump K-120 with 10 ml pump head,<br />
inert with ceramic inlays 1<br />
A41503 WellChrom <strong>HPLC</strong> Pump K-120 with 50 ml pump head,<br />
inert with ceramic inlays 1<br />
A0249 Biocompatible Titanium Capillary-Kit for pump head<br />
and pressure unit for A41501 and A41503 1<br />
Refer to page 22 for more information on pump heads and accessories.<br />
The <strong>HPLC</strong> pumps of the WellChrom family, in particular the K-120, K-501, and K-1001<br />
are delivered with the same mechanical drive. Thus, all pumps can be equipped with<br />
the same type of pump head. Spare parts can also be used with the K-100, K-500,<br />
and K-1000.<br />
Description<br />
The smallest pump in the world!<br />
Interchangeable inert pump heads for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Semi-preparative <strong>HPLC</strong><br />
Isocratic pump to promote a solvent<br />
composition. Up to four pumps can be<br />
used to receive a high pressure gradient<br />
system. Although no pressure indicator<br />
is included, an automatic pressure overflow<br />
control will stop the pump if 400 bar<br />
have been exceeded. A manometer is<br />
available upon request.<br />
This model is suitable as a dosage pump<br />
in preparative <strong>HPLC</strong> as well as for<br />
reagent feed in post-column derivatizations.<br />
Delivery System<br />
Double-piston pump with main and<br />
auxiliary piston<br />
Flow Ranges<br />
10 ml pump head: 0.001–9.999ml/min*<br />
50 m pump head:0.001–49.99ml/min<br />
* 3 digits with software control only!<br />
Flow Rate Accuracy<br />
≤ 0.5 % RSD (measured at 1 ml/min)<br />
Maximum Operating Pressure<br />
10 ml pump head: 400 bar<br />
50 ml pump head: 150 bar<br />
Control<br />
RS-232 connector, remote connection<br />
(start, stop)<br />
The pump can be controlled by<br />
EuroChrom ® 2000 V 1.65 and higher<br />
Display<br />
2 characters<br />
Dimensions and Weight<br />
105 x 100 x 185 mm (W x H x D),<br />
1.5 kg<br />
21
Pumps and <strong>Accessories</strong><br />
22<br />
<strong>Accessories</strong> and Spare Parts for WellChrom <strong>HPLC</strong> Pumps<br />
K-120, K-501, K-1001, K-100, K-500, and K-1000<br />
Order No. Article Quantity<br />
A4033 10 ml pump head, inert with ceramic inlays 1<br />
A1514 Set of gaskets, for 1/8” piston rod for A4033:<br />
1 seal high pressure side, 1 seal low pressure side, 1 O-ring 1<br />
A1515 Set of gaskets, for 1/8” piston rod preferably for aqueous solutions<br />
for A4033: 1 seal high pressure side, 1 seal low pressure side, 1 O-ring 1<br />
A1414 Set of gaskets, for 1/8” piston rod for A4033:<br />
2 seals high pressure side, 2 O-rings (can also be used on the low<br />
pressure side if the pump head is made of stainless steel or PEEK) 1<br />
A1410 Piston rod, 1/8”, for A4033 1<br />
Order No. Article Quantity<br />
A4034 50 ml pump head, inert with ceramic inlays 1<br />
A0981 Set of gaskets, for 1/4” piston rod for A4034:<br />
1 seal high pressure side, 1 seal low pressure side, 1 O-ring 1<br />
A0982 Set of gaskets, for 1/4” piston rod preferably for aqueous solutions,<br />
for A4034: 1 seal high pressure side, 1 seal low pressure side, 1 O-ring 1<br />
A0768 Set of gaskets, for 1/4” piston rod for A4034:<br />
2 seals high pressure side, 2 O-rings 1<br />
A1411 Piston rod, 1/4”, for A4034 1<br />
Order No. Article Quantity<br />
A2035 Cooling and heating device, for A4033 and A4034 1<br />
A1409 Pressure release valve, for A4033 and A4034 1<br />
A0863 Set of gaskets for check valve A0684, PEEK 1<br />
A0684 Check valve unit 1<br />
A0732 PTFE tubing, 1/8’’ x 1,5 mm, 3 m 1<br />
A0895 RS-232 cable to connect to a PC’s serial interface 1<br />
A1149 PTFE solvent filter, tubing included 1<br />
A1087 Bushing and seal, 1/8”, for pump head inlet 1<br />
A1476 Repair-Kit for A4033 includes: 1<br />
2 x A1410, 2 x A0684, 2 x A1514, 2 x A0863<br />
A1477 Repair-Kit for A4034 includes: 1<br />
2 x A1411, 2 x A0684, 2 x A0981, 2 x A0863
WellChrom Preparative <strong>HPLC</strong> Pump K-1800<br />
Ordering Information<br />
Order No. Article Quantity<br />
A40209 WellChrom Preparative <strong>HPLC</strong> Pump K-1800 with<br />
100 ml pump head, stainless steel 1<br />
A40201 WellChrom Preparative <strong>HPLC</strong> Pump K-1800 with<br />
250 ml pump head, stainless steel 1<br />
A40205 WellChrom Preparative <strong>HPLC</strong> Pump K-1800 with<br />
500 ml pump head, stainless steel<br />
A40202 WellChrom Preparative <strong>HPLC</strong> Pump K-1800 with<br />
1,000 ml pump head, stainless steel 1<br />
A4099 Valves for low pressure gradient for K-1800, stainless steel 1<br />
Refer to page 25 for more information on preparative pump heads and accessories.<br />
Description<br />
The »K-1800« is a flexible <strong>HPLC</strong> pump<br />
with an interchangeable pump head.<br />
This pump ensures constant flow rates<br />
for both analytical and preparative<br />
<strong>HPLC</strong> and for SMB purposes.<br />
Up to four pumps can be used to receive<br />
a high pressure gradient system.<br />
This pump demonstrates its reliable and<br />
reproducible gradient profiles, even<br />
with the optional low pressure gradient<br />
valve module.<br />
Delivery System<br />
Double-piston pump with Al2O3-pistons with electronic residual pulsation suppressor<br />
Flow Ranges<br />
100 ml pump head: 0.1 – 99.9 ml/min<br />
250 ml pump head: 0.1 – 249.9 ml/min<br />
1,000ml pump head: 0.1–999.9 ml/min<br />
Flow Rate Accuracy<br />
± 2 %, measured at 2 – 50 % of the<br />
flow range using methanol/water<br />
80 : 20 (v/v), RSD < 0.1 %<br />
Maximum Operating Pressure<br />
100 ml pump head: 400 bar<br />
250 ml pump head: 200 bar<br />
1,000 ml pump head: 50 bar<br />
System Protection<br />
Soft start with adjustable Pmin and Pmax Control<br />
2 RS-232 connectors, 8 event outputs<br />
(TTL, OC, relays), remote connection<br />
(start, error)<br />
The pump can be controlled with<br />
EuroChrom ® for Windows, Preparative<br />
Version V 1.65 and higher<br />
Display<br />
2 x 24 characters<br />
Power Supply<br />
115/230 V, 47 – 63 Hz, 450 W<br />
Dimensions and Weight<br />
220 x 185 x 340 mm (W x H x D),<br />
10.0 kg<br />
23
Pumps and <strong>Accessories</strong><br />
Description<br />
The »K-1900« is a pneumatic driven<br />
pump without electronics. Thus, the<br />
pump is suitable for work in hazardous<br />
environments. Due to its strong drive,<br />
it can reach flow rates of up to 2,000<br />
ml/min. This pump is ideal for column<br />
packing of microbore, analytical, and<br />
preparative columns that are under<br />
conditions up to 1,000 bar. The constant<br />
flow rate of the pump enables it<br />
to be used in process and production<br />
chemistry. Combined with the cooling<br />
option available, transportation of<br />
supercritical fluids is made possible.<br />
Due to its strong and powerful drive,<br />
the »K-1900« reaches much higher flow<br />
rates using the same pump heads as<br />
say, the WellChrom Preparative Pump<br />
K-1800. A long life and low service requirements<br />
are of course »included«.<br />
Operating Parameter<br />
100 ml pump head:<br />
Inlet air pressure: 4.2 bar<br />
Max. flow rate: 300 ml/min<br />
Max. solvent pressure: 1,000 bar<br />
Piston diameter: 6.35 mm<br />
Piston stroke volume/cycle: 0.6 ml<br />
Piston transformation ratio: 1 : 250<br />
250 ml pump head:<br />
Inlet air pressure: 7.0 bar<br />
Max. flow rate: 500 ml/min<br />
Max. solvent pressure: 670 bar<br />
Piston diameter: 9.525 mm<br />
Piston stroke volume/cycle: 1.35 ml<br />
Piston transformation ratio: 1 : 96<br />
1,000 ml pump head:<br />
Inlet air pressure: 7.0 bar<br />
Max. flow rate: 2,000 ml/min<br />
Max. solvent pressure: 160 bar<br />
Piston diameter: 19.05 mm<br />
Piston stroke volume/cycle: 5.4 ml<br />
Piston transformation ratio: 1 : 23<br />
Piston Stroke<br />
For all pump heads 9.5 mm<br />
Display<br />
Inlet air pressure, solvent pressure<br />
Dimensions and Weight<br />
220 x 185 x 340 mm (W x H x D),<br />
10.2 kg<br />
24<br />
WellChrom Pneumatic <strong>HPLC</strong> Pump K-1900<br />
Ordering Information<br />
Order No. Article Quantity<br />
A40289 WellChrom Pneumatic <strong>HPLC</strong> Pump K-1900 with<br />
100ml pump head, stainless steel 1<br />
A40281 WellChrom Pneumatic <strong>HPLC</strong> Pump K-1900 with<br />
250ml pump head, stainless steel 1<br />
A40285 WellChrom Pneumatic <strong>HPLC</strong> Pump K-1900 with<br />
500ml pump head, stainless steel 1<br />
A40282 WellChrom Pneumatic <strong>HPLC</strong> Pump K-1900 with<br />
1,000ml pump head, stainless steel 1<br />
Refer to page 25 for more information on pump heads and accessories.
Preparative Pump Heads and <strong>Accessories</strong><br />
Ordering Information<br />
Order No. Pump Heads with <strong>Accessories</strong> Quantity<br />
A4029 100 ml pump head, stainless steel 1<br />
A4021 250 ml pump head, stainless steel 1<br />
A4038 500 ml pump head, stainless steel 1<br />
A4022 1,000 ml pump head, stainless steel 1<br />
A4036 8-fold pump head, stainless steel<br />
A2034 Cooling device for pump heads A4029, A4021, A4022<br />
A4099 Ternary low pressure gradient, preparative 1<br />
Order No. <strong>Accessories</strong> and Spare Parts for 100 ml Pump Heads Quantity<br />
A0684 Check valve unit 1<br />
A0747 Piston rod, 1/4” 1<br />
A0746 2 piston seals and 1 O-ring 1<br />
A2003 Hydraulic connection set (10 tube fitting screws, 10 tube ferrules) 1<br />
A1121 Device for peak recycling and SMB applications,<br />
2 inlet valve housings, 1 T-connector, 1 fitting, 1 screw, 1 ferrule,<br />
1 left-hand and 1 right-hand connection tubing 1<br />
Order No. <strong>Accessories</strong> and Spare Parts for 250 ml Pump Heads Quantity<br />
A1080 Check valve unit 1<br />
A1017 Piston rod, 3/8” 1<br />
A1046 2 piston seals and 1 O-ring 1<br />
A2003 Set of hydraulic connection (10 tube fitting screws, 10 tube ferrules) 1<br />
A1117 Device for peak recycling and SMB applications,<br />
2 inlet valve housings, 1 T-connector, 1 fitting, 1 screw, 1 ferrule,<br />
1 left-hand and 1 right-hand connection tubing 1<br />
Order No. <strong>Accessories</strong> and Spare Parts for 500 ml Pump Heads Quantity<br />
A1080 Check valve unit 1<br />
A1478 Piston rod, 9/16’’ 1<br />
A1479 2 piston seals and 1 O-ring 1<br />
Order No. <strong>Accessories</strong> and Spare Parts for 1,000 ml pump heads Quantity<br />
A1080 Check valve unit 1<br />
A1015 Piston rod, 3/4” 1<br />
A1077 2 piston seals and 1 O-ring 1<br />
Important Note<br />
Cooling and heating options for pump<br />
heads as well as connections to thermostats<br />
are available upon request.<br />
25
Pumps and <strong>Accessories</strong><br />
Description<br />
<strong>KNAUER</strong>’s Solvent Organizer replenishes<br />
the WellChrom <strong>HPLC</strong> Pump K-1001 to<br />
a low pressure gradient system that can<br />
process up to four solvents. The device<br />
is controlled by the pump and can be<br />
used with a maximum flow rate of 50<br />
ml/min. Due to inert pump heads combined<br />
with both a static mixing chamber<br />
and a PEEK valve unit, biocompatibility<br />
is guaranteed.<br />
Solvents<br />
Maximum 4<br />
Max Flow Rate per Channel<br />
50 ml/min<br />
Mixing Chamber Volume<br />
0.6 ml<br />
Control<br />
Controlled by WellChrom <strong>HPLC</strong> Pump<br />
K-1001<br />
Dimensions<br />
Container for bottles:<br />
210 x 40 x 300 mm (W x H x D)<br />
Container for tools:<br />
210x20x45mm (WxHxD)<br />
Overall dimensions:<br />
235 x 103 x 390 mm (W x H x D)<br />
Weight<br />
3.6 kg, 2.9 kg (PEEK)<br />
Important Note<br />
If the WellChrom Solvent Organizer<br />
K-1500 is operated with a static mixing<br />
chamber the flow rate should not<br />
exceed 1 ml/min.<br />
26<br />
WellChrom Solvent Organizer K-1500<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4012 WellChrom Solvent Organizer K-1500 for low pressure<br />
gradient systems, stainless steel 1<br />
A4013 WellChrom Solvent Organizer K-1500, PEEK 1<br />
A4016 Solvent Box without low pressure gradient valve unit,<br />
stainless steel 1
Detectors and <strong>Accessories</strong><br />
WellChrom Diode Array Detector K-2700<br />
Ordering Information<br />
Order No. Article Quantity<br />
A41121 WellChrom Diode Array Detector K-2700 1<br />
Consists of:<br />
A4112 DAD Module K-2700 1<br />
A4111 Deuterium Lamp Module K-2701 1<br />
A4098 Flow Cell, 10 mm, 1/16” 1<br />
Fiber Optics and <strong>Accessories</strong> 1<br />
A4113 Tungsten-Halogen Lamp 1<br />
Refer to page 32 and 33 for more information on flow cells and accessories.<br />
Description<br />
The WellChrom Diode Array Detector<br />
K-2700 gathers chromatographic data<br />
three dimensionally by measuring adsorption<br />
both as a function of retention<br />
time and wavelength. Thus, the optimal<br />
detection wavelength can be determined<br />
for each compound leading to<br />
the »best chromatogram«.<br />
The Diode Array Detector enables the<br />
use of spectra libraries for determining<br />
peak purity.<br />
Simultaneous measurement of chromatograms<br />
can be performed with a high<br />
sensitivity.<br />
Diode array and lamp modules are<br />
connected via fiber optics.<br />
Diode Array<br />
1024 diodes in a ceramic chassis<br />
Wavelength Ranges<br />
190 – 740 nm (Deuterium)<br />
400 – 1020 nm (Tungsten-Halogen)<br />
Light Source<br />
Deuterium lamp or<br />
Tungsten-Halogen lamp<br />
Spectral Resolution<br />
2 nm (0.8 nm pixel distance)<br />
Wavelength Accuracy<br />
± 0.1 nm<br />
Wavelength Reproducibility<br />
± 0.07 nm<br />
Noise<br />
5x10-5AU, typical value: 3 – 10 x 10-5 AU<br />
Sensitivity<br />
1x10-4AU Drift<br />
< 1 x 10-3 AU/h<br />
Control<br />
3D graphic interface and spectra library<br />
functions are included in EuroChrom ®<br />
2000 Basic Edition V 2.05 and Chrom-<br />
Gate ® software<br />
Recommended: Pentium PC with a minimum<br />
of 10 GB hard disk, 128 MB RAM,<br />
and 1 free PCI-slot for a RS-422 interface<br />
card per module<br />
Dimensions and Weight<br />
106 x 185 x 340 (W x H x D),<br />
6.7 kg for each module<br />
27
Detectors and <strong>Accessories</strong><br />
Description<br />
This spectro-photometer operates with<br />
interchangeable flow cells for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Preparative <strong>HPLC</strong><br />
Multiple chromatograms can be simultaneously<br />
acquired at different wavelengths.<br />
In the »stand-alone« mode the<br />
detector offers two adjustable wavelengths<br />
per run, while four wavelengths<br />
are available if externally controlled.<br />
In the »on-flow« mode UV-spectras can<br />
be acquired.<br />
The detector provides GLP functions<br />
such as: detailed reports, displaying the<br />
total hours of lamp operation, total<br />
number of lamp ignitions, and more.<br />
Wavelength Ranges<br />
190 – 740 nm (Deuterium)<br />
Edge filter (2nd order), 370 nm<br />
Light Source<br />
Deuterium lamp<br />
Optional Tungsten-Halogen lamp<br />
Band Width / Wavelength Accuracy<br />
∆λ ≤ 8 nm / ± 1nm<br />
Drift<br />
< 1 x 10-4 AU/h at 240 nm, t = 1s<br />
Noise<br />
< 1 x 10-5 AU at 254 nm, t = 1s<br />
With fiber optics:<br />
< 8 x 10-5 AU at 254 nm, t = 1s<br />
Sensitivity<br />
< 2 x 10-5 AU at 254 nm, t = 1s<br />
Scan Speed<br />
Complete wavelength range on-flow<br />
with ca. 100 nm/s<br />
Control<br />
2 RS-232 connectors, analog input,<br />
analog outputs (TTL, OC, relays), signal<br />
output for fraction collector, controlled<br />
by EuroChrom ® 2000 Basic Edition<br />
V 1.65 or higher and ChromGate ®<br />
Integrator Output<br />
± 0.1 V / ± 1 V / ± 10 V<br />
Dimensions and Weight<br />
160 x 185 x 340 mm (W x H x D),<br />
5.5 kg<br />
28<br />
WellChrom Fast Scanning Spectro-Photometer K-2600<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4070 WellChrom Fast Scanning Spectro-Photometer K-2600,<br />
without flow cell, accessories included 1<br />
Refer to page 32 and 33 for additional accessories and spare parts!
WellChrom Spectro-Photometer K-2501<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4180 WellChrom Spectro-Photometer K-2501,<br />
without flow cell, accessories included 1<br />
Refer to page 32 and 33 for additional accessories and spare parts!<br />
Description<br />
The WellChrom Spectro-Photometer<br />
K-2501 operates with interchangeable<br />
flow cells for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Preparative <strong>HPLC</strong><br />
For each run, a particular wavelength<br />
can be set between 200nm and 740nm.<br />
The wavelength can be changed during<br />
the measurement. Thus, different wavelengths<br />
for different runs can be easily<br />
programmed. When switched into the<br />
stop-flow mode, the detector is capable<br />
of acquiring UV-spectras.<br />
Wavelength Ranges<br />
190 – 740 nm (Deuterium)<br />
Edge filter (2nd order), 370 nm<br />
Light Source<br />
Deuterium lamp<br />
Optional Tungsten-Halogen lamp<br />
Band Width / Wavelength Accuracy<br />
∆λ ≤ 8 nm / ± 2 nm<br />
Drift<br />
< 15 x 10-5 AU/h bei 254 nm, t = 1s<br />
Noise<br />
< 1 x 10-5 AU at 254 nm, t = 1s<br />
Sensitivity<br />
2x10-5AU at 254 nm, t = 1s<br />
Scan Speed<br />
Complete wavelength range on-flow<br />
with ≈ 100 nm/s<br />
Digital Output<br />
RS-232 connector<br />
Analog Output<br />
± 1V<br />
GLP Functions<br />
Detailed reports, total lamp operation<br />
time, number of lamp ignitions<br />
Dimensions and Weight<br />
106 x 185 x 340 mm (W x H x D),<br />
4.0 kg<br />
29
Detectors and <strong>Accessories</strong><br />
Description<br />
This filter-photometer operates with<br />
interchangeable flow cells for<br />
• Micro <strong>HPLC</strong><br />
• Analytical <strong>HPLC</strong><br />
• Preparative <strong>HPLC</strong><br />
Depending on the filter used, chromatograms<br />
can be acquired at a fixed<br />
wavelength of 200nm, 220nm, 254nm,<br />
or 280 nm. Further wavelength filters<br />
are available upon request!<br />
An automatic change of the filter in<br />
use can be programmed.<br />
Wavelength<br />
Up to 6 standard 1/2” filters are possible.<br />
The standard delivery contains<br />
4 filters: 200 nm, 220 nm, 254 nm,<br />
and 280 nm.<br />
Light Source<br />
Deuterium lamp<br />
Optional Tungsten-Halogen lamp<br />
Band Width<br />
∆λ ≤ 20 nm<br />
Drift<br />
< 15 x 10-5 AU/h at 254 nm, t = 1s<br />
Noise<br />
< 1 x 10-5 AU at 254 nm, t = 1s<br />
Sensitivity<br />
2x10-5AU at 254 nm, t = 1s<br />
Digital Output<br />
RS-232 connector<br />
Analog Output<br />
±1V<br />
GLP Functions<br />
Detailed reports, total lamp operation<br />
time, number of lamp ignitions<br />
Dimensions and Weight<br />
106 x 185 x 340 mm (W x H x D),<br />
4.0 kg<br />
30<br />
WellChrom Filter-Photometer K-2001<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4160 WellChrom Filter-Photometer K-2001,<br />
flow cell excluded, accessories included 1<br />
Refer to page 32 and 33 for additional accessories and spare parts!
WellChrom Spectro-Photometers K-2001, K-2501, and K-2600<br />
with Fiber Optics<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4200 WellChrom Filter-Photometer K-2001,<br />
with fiber optics, flow cell excluded, accessories included 1<br />
A4210 WellChrom Spectro-Photometer K-2501,<br />
with fiber optics, flow cell excluded, accessories included 1<br />
A4090 WellChrom Fast Scanning Spectro-Photometer K-2600,<br />
with fiber optics, flow cell excluded, accessories included 1<br />
Refer to page 32 and 33 for additional accessories and spare parts!<br />
Description<br />
The use of fiber optics enables physical<br />
separation of the flow cell from<br />
the detector hardware. Thus, spectrophotometers<br />
are ideal for use in hazardous<br />
environments or near explosive<br />
materials. The temperature of the flow<br />
cell is adjustable. All spectro-photometers<br />
in the WellChrom family can<br />
be equipped with fiber optics.<br />
31
Detectors and <strong>Accessories</strong><br />
Flow Cells, <strong>Accessories</strong>, and Spare Parts<br />
Ordering Information<br />
32<br />
Analytical Flow Cells for WellChrom K-200, K-2001, K-2501, and K2600<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max.Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4061 10 (1/16’’) 1.1 10 stainless steel* 20 300<br />
A4042 3 (1/16’’) 1 2 stainless steel 50 300<br />
A4045 3 (1/16’’) 1 2 PEEK 50 30<br />
Analytical Flow Cells for WellChrom Diode Array Detector K-2700<br />
– single fiber optical connector –<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max.Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4098 10 (1/16’’) 1.1 10 stainless steel* 20 300<br />
A4043 3 (1/16’’) 1 2 stainless steel 50 300<br />
A4046 3 (1/16’’) 1 2 PEEK 50 30<br />
Preparative Flow Cells for WellChrom K-200, K-2001, K-2501, and K2600<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max.Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4066 0.5/1.25/2 (1/8’’) stainless steel 1,000 200<br />
A4067 0.5/1.25/2 (1/8’’) PEEK 1,000 100<br />
A4068 0.5/1.25/2 (1/4’’) stainless steel 10,000 200<br />
A4069 0.5 (1/16’’) stainless steel 250 200<br />
A4095 0.5 (1/16’’) PEEK 250 100<br />
Preparative Flow Cells for WellChrom Diode Array Detector K-2700<br />
– single fiber optical connector –<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max.Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4117 0.5/1.25/2 (1/8’’) stainless steel 1,000 200<br />
A4119 0.5/1.25/2 (1/8’’) PEEK 1,000 100<br />
A4120 0.5/1.25/2 (1/4’’) stainless steel 10,000 200<br />
A4121 0.5 (1/16’’) stainless steel 250 200<br />
A4122 0.5 (1/16’’) PEEK 250 100<br />
* with heat exchanger
Flow Cells, <strong>Accessories</strong>, and Spare Parts<br />
Ordering Information<br />
Analytical Flow Cells for WellChrom K-200, K-2001, K-2501, and K2600<br />
– double fiber optical connection –<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max. Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4074 10 (1/16’’) 1.1 10 stainless steel* 20 300<br />
A4044 3 (1/16’’) 1 2 stainless steel 50 300<br />
A4047 3 (1/16’’) 1 2 PEEK 50 30<br />
A4077 3 (1/16’’) 0.7 1 PEEK 10 30<br />
A4078 0.5/1.25/2 (1/8’’) stainless steel 1,000 200<br />
A4079 0.5/1.25/2 (1/8’’) PEEK 1,000 100<br />
A4081 0.5/1.25/2 (1/4’’) stainless steel 10,000 200<br />
A4089 0.5 (1/16’’) stainless steel 250 200<br />
A4096 0.5 (1/16’’) PEEK 250 100<br />
U-Z View Micro Flow Cells for WellChrom K-200, K-2501, and K2600<br />
– fiber optics not available –<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max. Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4091 8 (1/16’’) 0.150 0.140 fused silica 0.1 500<br />
A4044 8 (1/16’’) 0.075 0.035 fused silica 0.01 500<br />
A4047 8 (1/16’’) 0.020 0.003 fused silica 0.001 500<br />
CE Cell (Capillary Electrophoresis)<br />
– fiber optics not available –<br />
Order No. Layer Thickness ID Vol. Material Flow Rate Max. Pressure<br />
mm (connection) mm µl ml/min bar<br />
A4097 1 (1/16’’) stainless steel<br />
* with heat exchanger<br />
Important Note<br />
Cell volumes for adjustable cells are:<br />
0.50 mm � 1.7 µl<br />
1.25 mm � 4.3 µl<br />
2.00 mm � 6.8 µl<br />
33
Detectors and <strong>Accessories</strong><br />
Important Note<br />
Each repair kit (A1131, A1475, and<br />
A1132) for flow cells consist of 2 seals,<br />
2 gasket holders, and 2 lenses.<br />
34<br />
Flow Cells, Spare Parts, <strong>Accessories</strong> for Photometric Detectors<br />
Ordering Information<br />
Order No. Article Quantity<br />
A4071-1 Deuterium lamp (35 W) 1<br />
A4072 Tungsten-Halogen lamp 1<br />
A4073 Tungsten-Halogen lamp with base (required for first time installation) 1<br />
A4142 Mercury vapor lamp with lamp board for<br />
WellChrom Fixed Wavelength Detector K-200 1<br />
A4098 Analytical flow cell, 10 mm, 10 µl, stainless steel,<br />
with connector for fiber optics 1<br />
A0911 Fiber optical connector, length 400 mm 1<br />
A0742 Fiber optical connector, length 750 mm 1<br />
A0740 Fiber optical connectors, length 750 mm 2<br />
A0743 Fiber optical connectors with individual length upon request 1<br />
A0857 Extension-Kit for coupling two fiber optical connectors<br />
to separate the cell from the instrument hardware 1<br />
A4126 Test cell, holmium oxide filter for photometers 1<br />
A4127 Test cell, holmium oxide filter for<br />
WellChrom Diode Array Detector K-2700 1<br />
A4128 Test cell, holmium oxide filter for detectors using fiber optics 1<br />
A1447 1 lens, 1 seal for analytical flow cells 1<br />
A1448 1 lens, 1 seal for preparative flow cells 1<br />
A1449 1 lens, 1 seal for flow cells using fiber optics 1<br />
A1131 Repair-Kit for analytical flow cells, 10 mm 1<br />
A1475 Repair-Kit for analytical flow cells, 3 mm 1<br />
A1132 Repair-Kit for preparative flow cells 1<br />
A2004 PCI interface card for WellChrom Diode Array Detector K-2700 1
WellChrom Differential Refractometers K-2301 and K-2401 Description<br />
Ordering Information<br />
Order No. Article Quantity<br />
A2110 WellChrom Differential Refractometer K-2301, analytical,<br />
flow cell A0294 and accessories included 1<br />
A2111 WellChrom Differential Refractometer K-2401, preparative,<br />
flow cell A0287 and accessories included 1<br />
Order No. <strong>Accessories</strong> and Spare Parts Quantity<br />
A0294 Flow cell, 45 °, 9 µl 1<br />
A0287 Flow cell, 15 °, 12 µl, sensitivity reduced by factor 3 1<br />
A0277 Gasket for flow cell A0294 and A0287 1<br />
All WellChrom Differential Refractometers<br />
are suitable for detecting little<br />
or no UV-active compounds such as<br />
sugars or polymers. The instruments<br />
are designed for use in analytical and<br />
preparative <strong>HPLC</strong> as well as for GPC<br />
applications.<br />
WellChrom<br />
Differential Refractometer K-2301<br />
Wavelength<br />
950 ± 30 nm<br />
Range<br />
±1x10-3∆n Sensitivity<br />
< 8 x 10-8 ∆n<br />
Noise<br />
≤ 4x10-8∆n Cell Angle and Volume<br />
45 °, 9 µl<br />
Max. Flow Range<br />
5 ml/min<br />
WellChrom<br />
Differential Refractometer K-2401<br />
Wavelength<br />
950 ± 30 nm<br />
Range<br />
±2x10-3∆n Sensitivity<br />
8x10-7∆n Noise<br />
< 4 x 10-7 ∆n<br />
Cell Angle and Volume<br />
15 °, 12 µl<br />
Max. Flow Range<br />
99 ml/min<br />
Autozero<br />
Full range<br />
Analog Output<br />
±1 V<br />
Digital Output<br />
RS-232 connector<br />
Various<br />
Internal selenoid valve for purging<br />
the reference cell<br />
Dimensions and Weight<br />
106 x 185 x 340 mm (W x H x D),<br />
8.0 kg<br />
35
Detectors and <strong>Accessories</strong><br />
Description<br />
Fluorescence <strong>HPLC</strong> Monitor with high<br />
sensitivity. Thus, suitable for amino acids<br />
and other compounds showing fluorescence<br />
activity, particularly in trace analysis.<br />
Light Source<br />
Xenon lamp, 150 W<br />
Monochromator<br />
Concave, holographic grating monochromator<br />
Wavelength Range<br />
200 – 650 nm (optional 200 – 900 nm)<br />
Spectral Bandwidth<br />
15 nm, in excitation and emission state<br />
Wavelength Accuracy<br />
±2nm<br />
Wavelength Reproducibility<br />
±0.2 nm<br />
Sensitivity<br />
The signal-noise ratio for the Raman<br />
band of distilled water is 300<br />
(excitation at 350 nm, t = 1.5 s)<br />
Detection Unit<br />
Photomultiplier<br />
Cell Volume, Pressure, Materials<br />
Standard:<br />
12 µl, 2 mPa SS316, Silica, and PTFE<br />
Optional:<br />
2 µl and inert cells are available<br />
upon request<br />
Wavelength Scanning<br />
Available for exitation and emission<br />
wavelength<br />
Time Programming<br />
Up to 32 steps for wavelength settings<br />
Temperature Range<br />
4–35°C<br />
Power Supply<br />
100 – 240 V, 350 VA, 50/60 Hz<br />
Dimensions and Weight<br />
260 x 205 x 520 mm (W x H x W),<br />
17.0 kg<br />
36<br />
Fluorescence <strong>HPLC</strong> Monitor RF-10 AXL<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0815 Fluorescence <strong>HPLC</strong> Monitor RF-10 AXL 1<br />
A0753 Xenon lamp RF-10, 150 W 1
Conductivity Detector for Ion Chromatography<br />
Model 550<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1252 Conductivity Detector Model 550 1<br />
Description<br />
Suitable for determining ionic compounds.<br />
High sensitivity due to an<br />
actively tempered conductivity cell.<br />
An integrated heat exchanger preheats<br />
the mobile phase to cell temperature.<br />
The suppressor ERIS ® 1000 guarantees<br />
optimal chemical conditions for excellent<br />
results.<br />
The detector offers a wide range of<br />
linearity. Concentrations of up to<br />
500 mg/l can be directly measured<br />
enabling high reproducibility.<br />
All functions are easy to use.<br />
Measuring Range<br />
0.01 – 5,000 µS/cm in 12 steps<br />
Noise<br />
0.0004 µS/cm<br />
(double distilled water, 1ml/min, 35 °C)<br />
Baseline Drift<br />
< 0.004 µS/h<br />
(double distilled water, 1ml/min, 35 °C)<br />
Cell Volume<br />
0.5 µl<br />
Analog Output<br />
10 mV / 100 mV / 1000 mV<br />
Dimensions and Weight<br />
69 x 62 x 158 mm (W x H x D),<br />
5.9 kg<br />
37
Detectors and <strong>Accessories</strong><br />
Description<br />
The highly sensitive Amperometric<br />
Detector L-3500 determines catecholamines.<br />
The powerful electronic measuring unit<br />
and optimized cell are outstanding<br />
characteristics of this detector.<br />
Suitable for analytical and narrow-bore<br />
<strong>HPLC</strong> in clinical and pharmacological<br />
applications. Its fantastic signal-to-noise<br />
ratio enables remarkable detection<br />
limits. Because the detector performs<br />
an autozero at the beginning of each<br />
chromatogram, it can be used for<br />
routine analysis without supervision.<br />
Cell Volume<br />
1.5 µl, optional available are 0.75 µl<br />
and 2.5 µl<br />
Voltage<br />
0.000 to ± 1.999 V<br />
Input Current Range<br />
0 to ± 200 nA<br />
Measuring Range<br />
0.02 – 50 nA FS<br />
Offset Compensation<br />
± 200 nA, accuracy better<br />
than 0.05 % RSD<br />
Dimensions<br />
Electronic unit:<br />
255 x 105 x 310 mm (W x H x D)<br />
Measuring cell:<br />
120 x 155 x 110 mm (W x H x D)<br />
Weight<br />
5.5 kg (cell included)<br />
38<br />
Amperometric Detector L-3500<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1186 Amperometric Detector L-3500 with measuring cell 1
Light Scattering Detector ELSD 2000<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0925 Light Scattering Detector ELSD 2000 1<br />
Description<br />
A highly sensitive, universal, and neutral<br />
detector for the determination of compounds<br />
with little or no UV-activity such<br />
as: sugars, fats, triglycerides, phosphoric<br />
lipides, detergents, polymers, natural<br />
substances, pharmaceutical compounds<br />
or amino acids, and peptides.<br />
The molecular characteristic of light<br />
scattering is set to be directly proportional<br />
to that of the mole mass.<br />
Because of its drift-free detection as<br />
well as its independent gradient choice,<br />
the ELSD 2000 offers a great variety of<br />
applications in micro <strong>HPLC</strong>, combinatorial<br />
chemistry, and GPC.<br />
Flow Control<br />
Digital gas flow control up to 5 l/min<br />
nitrogen preferred!<br />
Pressure<br />
Maximum 80 psi<br />
Temperature<br />
Room temperature – 120 °C<br />
in steps of 1 °C<br />
Analog Output<br />
0–1V/0–10mV<br />
Display<br />
Graphical display<br />
Dimensions and Weight<br />
590 x 320 x 550 mm (W x H x D),<br />
16.0 kg<br />
39
<strong>HPLC</strong> Columns<br />
and Electric Valve Drives<br />
Description<br />
For column switching, sample enrichment<br />
on separate adsorption units,<br />
and fraction collection.<br />
The WellChrom Electric Valve Drive K-6<br />
can be used for 1 x 60 ° and 6 x 60 ° Injection<br />
and Switching Valves.<br />
The WellChrom Electric Valve Drive K-16<br />
can be used for 17-Port/1-Channel Injection<br />
and Switching Valves,<br />
16 x 22.5 °<br />
Switching Time<br />
< 150 ms<br />
Display<br />
2 characters<br />
Control<br />
RS-232 connector, external short<br />
circuit, binary code<br />
Dimensions and Weight<br />
85 x 100 x 150 mm (W x H x D),<br />
0.8 kg<br />
40<br />
WellChrom Electric Valve Drives K-6 and K-16<br />
Ordering Information<br />
WellChrom Electric Valve Drive K-6, incl. external power supply and a<br />
6-Port/3-Channel Injection and Switching Valve<br />
Order No. Maximum pressure: 300 bar Quantity<br />
A1484 Stainless steel, 1/16” 1<br />
A1485 PEEK, 1/16” 1<br />
A1486 Stainless steel, 1/8” 1<br />
A1487 PEEK, 1/8” 1<br />
7-Port/1-Channel Switching Valve<br />
A1488 Stainless steel, 1/16” 1<br />
A1489 PEEK, 1/16’’ 1<br />
A1490 Stainless steel, 1/8” 1<br />
A1491 PEEK, 1/8’’ 1<br />
WellChrom Electric Valve Drive K-16, incl. external power supply and a<br />
Order No. 17-Port/1-Channel Switching Valve Quantity<br />
A1492 Stainless steel, 1/16”, maximum pressure 50 bar 1<br />
A1492-1 Stainless steel, 1/16”, maximum pressure 100 bar 1<br />
Order No. <strong>Accessories</strong> and Spare Parts Quantity<br />
A0377 WellChrom Electric Valve Drive K-6, with external power supply,<br />
valve excluded 1<br />
A0782 WellChrom Electric Valve Drive K-16, with external power supply,<br />
valve excluded 1<br />
A0895 RS-232 cable for connection to a PC 1
Injection and Switching Valves for K-6 and K-16<br />
Ordering Information<br />
Order No. 6-Port/3-Channel Injection and Switching Valves for K-6 Quantity<br />
A1369 Stainless steel, 1/16” 1<br />
A1370 PEEK, 1/16” 1<br />
A1371 Stainless steel, 1/8” 1<br />
A1372 PEEK, 1/8”<br />
Order No. 6-Port/2-Channel Switching Valve for K-6 Quantity<br />
A1369-1 Stainless steel, 1/16” 1<br />
Order No. 7-Port/1-Channel Switching Valves for K-6 Quantity<br />
A1373 Stainless steel, 1/16” 1<br />
A1374 PEEK, 1/16” 1<br />
A1375 Stainless steel, 1/8” 1<br />
A1376 PEEK, 1/8” 1<br />
Order No. 17-Port/1-Channel Switching Valves for K-16 Quantity<br />
A1379 Stainless steel, 1/16”, pressure stable up to 50 bar 1<br />
A1379-1 Stainless steel, 1/16”, pressure stable up to 100 bar 1<br />
Manually Driven Injection and Switching Valves<br />
Ordering Information<br />
Order No. 6-Port/2-Channel Injection Valves Quantity<br />
A1365 Stainless steel, 1/16” 1<br />
A1366 PEEK, 1/16” 1<br />
Order No. 6-Port/3-Channel Injection Valves Quantity<br />
A1357 Stainless steel, 1/16” 1<br />
A1358 PEEK, 1/16” 1<br />
A1359 Stainless steel, 1/8” 1<br />
A1360 PEEK, 1/8” 1<br />
Order No. 7-Port/1-Channel Switching Valves Quantity<br />
A1361 Stainless steel, 1/16” 1<br />
A1362 PEEK, 1/16” 1<br />
A1363 Stainless steel, 1/8” 1<br />
A1364 PEEK, 1/8” 1<br />
Order No. <strong>Accessories</strong> Quantity<br />
A4018 Universal Mounting Bracket 1<br />
A0406 Universal Mounting Bracket for WellChrom <strong>HPLC</strong> Pump K-120 1<br />
Important Note<br />
Valves made out of stainless steel are<br />
pressure stable up to 300 bar.<br />
Valves made out of PEEK are pressure<br />
stable up to 200 bar.<br />
41
Automated<br />
<strong>HPLC</strong> Injection Systems<br />
Description<br />
The sample carousel of the WellChrom<br />
Basic Marathon K-3800 is accessible at<br />
anytime during operation. Due to the<br />
combination of sequence and method<br />
programming, automatic injection<br />
and online sample preparation can<br />
be easily achieved. The device can<br />
be controlled by EuroChrom ® 2000<br />
Basic Edition V 1.65 or higher or by<br />
ChromGate ® .<br />
An option for tempering the sample<br />
carousel is available upon request.<br />
Sample Capacity<br />
96 sample vials of 1.8 ml or<br />
48 sample vials of 5.0 ml<br />
Injection Method<br />
Total sample loop filling<br />
Partial sample loop filling is optional<br />
Sample Loops<br />
100 µl (default), 250 µl, 500 µl,<br />
1,000 µl<br />
Reproducibility<br />
< 0.5 % RSD<br />
Control<br />
RS-232 connector, controlled by<br />
EuroChrom ® 2000 Basic Edition V 1.65<br />
or higher or by ChromGate ®<br />
Dimensions and Weight<br />
280 x 440 x 540 mm (W x H x D),<br />
15.0 kg<br />
42<br />
Autosampler WellChrom Basic Marathon K-3800<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0759 WellChrom Basic Marathon K-3800 1<br />
A07591 WellChrom Basic Marathon K-3800 COOL, with cooling 1<br />
A07592 WellChrom Basic Marathon K-3800 PLUS,<br />
partial sample loop filling 1<br />
<strong>Accessories</strong> for WellChrom Autosampler Basic Marathon K-3800<br />
Order No. and TRIATHLON Quantity<br />
A0664 Accessory kit for WellChrom Autosampler Basic Marathon K-3800<br />
and Autosampler TRIATHLON 1<br />
A0638 Sample vials, caps, septum caps, ∅ 11 mm 1,000<br />
A0865 Pliers for opening septum caps, ∅ 11 mm 1<br />
A0864 Pliers for closing septum caps, ∅ 11 mm 1
Autosampler TRIATHLON Description<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0320 Autosampler TRIATHLON 1<br />
A03201 Autosampler TRIATHLON COOL 1<br />
A03202 Autosampler TRIATHLON, preparative version 1<br />
<strong>Accessories</strong> for WellChrom Autosampler Basic Marathon K-3800<br />
Order No. and TRIATHLON Quantity<br />
A0664 Accessory kit for WellChrom Autosampler Basic Marathon K-3800<br />
and Autosampler TRIATHLON 1<br />
A0638 Sample vials, caps, septum caps, ∅ 11 mm 1,000<br />
A0865 Pliers for opening septum caps, ∅ 11 mm 1<br />
A0864 Pliers for closing septum caps, ∅ 11 mm 1<br />
A3440 Preparative kit for autosampler consisting of<br />
10 ml autosampler syringe, 10 ml sample loop stainless steel,<br />
15 ml buffer tubing for pre-column derivatization 1<br />
The Autosampler TRIATHLON’s high<br />
injection precision as well as its micro<br />
handling are a few of the many reasons<br />
why it is suitable for routine analysis.<br />
Fast injection cycles and reliable sample<br />
preparation steps such as enrichment,<br />
dilution, and derivatization using the<br />
smallest sample volumes is possible.<br />
These are some of the outstanding<br />
characteristics of the device making it<br />
a real advantage to have in the lab.<br />
Sample Capacity<br />
96 Sample vials of 1.5 ml or<br />
72 Sample vials of 4.0 ml or<br />
32 Sample vials of 10.0 ml each<br />
Injection Method<br />
Full and partial sample loop filling,<br />
µl-injection<br />
Injection Volumes<br />
1 to 1,000 µl in steps of 1 µl<br />
Maximum Run Time<br />
10 h<br />
Reproducibility<br />
< 0.3 % RSD at full sample loop filling<br />
Sample Loops<br />
100 µl, 250 µl (default), 500 µl, 1,000 µl<br />
Control<br />
RS-232 connector, 5 signal outputs,<br />
1 signal input, works as master or slave<br />
Options<br />
Cooling unit, 4 – 40 °C<br />
Biocompatible injection valve<br />
Integrated solvent valve<br />
Power Supply<br />
115/220 V, 50/60 Hz<br />
Dimensions and Weight<br />
280 x 440 x 540 mm (W x H x D),<br />
22.0 kg<br />
43
Automated<br />
<strong>HPLC</strong> Injection Systems<br />
Description<br />
Enables high sample throughput with<br />
the smallest sample volume. The injection<br />
can be performed directly from<br />
sample vials or micro titre plates.<br />
Sample Capacity<br />
1 titre plate 128 x 86 mm plus<br />
4 sample vials of 10 ml<br />
Injection Method<br />
Full and partial sample loop filling<br />
Injection Volumes<br />
1 to 1,000 µl steps of 1 µl<br />
Sample Loops<br />
100 µl, 250 µl (default), 500 µl, 1,000 µl<br />
Options<br />
Cooling unit, 4 – 40 °C,<br />
Biocompatible injection valve<br />
Mounting bracket for additional micro<br />
titre plates with 96 positions each<br />
Power Supply<br />
115/220 V, 50/60 Hz<br />
Dimensions and Weight<br />
280 x 440 x 400 mm (W x H x D), 22.0 kg<br />
Description<br />
The Autosampler MIDAS meets both<br />
analytical and preparative requirements<br />
in your lab!<br />
Sample Capacity<br />
Sample carousel with 84 sample vials<br />
of 1.5 ml plus 3 vials of 10 ml for additional<br />
liquids<br />
Injection Method<br />
Full and partial sample loop filling<br />
Injection Volumes<br />
1 µl to 5,000 µl<br />
Sample Loops<br />
250 µl (default), 500 µl, 5,000 µl<br />
Options<br />
Cooling unit, 4 – 40 °C, biocompatible<br />
injection valve, sample carousel for<br />
1.5 ml and 10 ml vials.<br />
Power Supply<br />
115/220 V, 50/60 Hz<br />
Dimensions and Weight<br />
280 x 440 x 400 mm (W x H x D),<br />
22.0 kg<br />
44<br />
Autosampler ENDURANCE<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1500 Autosampler ENDURANCE 1<br />
A15001 Autosampler ENDURANCE COOL, with sample cooling 1<br />
Autosampler MIDAS<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1508 Autosampler MIDAS, analytical version 1<br />
A15081 Autosampler MIDAS COOL, analytical version with sample cooling 1<br />
A15082 Autosampler MIDAS COOL, preparative version 1
Fraction Collectors<br />
Fraction Collector Retreiver 500<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1183 Fraction Collector RETREIVER 500, set of racks for collectors<br />
of 12 – 13 mm ID included 1<br />
A1249 Safety valve for flow interruption 1<br />
A1250 Diverter valve for waste flow control 1<br />
Fraction Collector Foxy Jr.<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1170 Fraction Collector FOXY JR., rack included, 13 mm 1<br />
A1284 Safety valve for flow interruption 1<br />
A1273 Diverter valve for waste flow control 1<br />
Fraction Collector B-684<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1437 Fraction Collector B-684, racks excluded 1<br />
A1438 Set of racks, 120 x 50 ml collectors 1<br />
A1498 Set of racks, 120 x 20 ml collectors 1<br />
A1499 Set of racks, 48 x 250 ml collectors 1<br />
Description<br />
The Fraction Collector RETREIVER 500,<br />
applied in preparative chromatography,<br />
holds a maximum of 102 collectors (ID<br />
12 – 13 mm). Fraction collection can be<br />
performed according to time, drop<br />
number, and volume control.<br />
Valves are excluded from the standard<br />
delivery!<br />
Temperature Range 0–40°C<br />
Weight 3.0 kg<br />
Description<br />
Suitable for preparative chromatography.<br />
Holds a maximum of 144 collectors<br />
(12 – 13 mm ID). Fraction collection can<br />
be performed according to time, drop<br />
number, and volume control.<br />
Peak detection according to time<br />
window, signal level, and signal slope.<br />
Valves are excluded from the standard<br />
delivery!<br />
Temperature Range 0–40°C<br />
Weight 4.7 kg<br />
Description<br />
The »B-684« collects substances after<br />
their separation on the <strong>HPLC</strong> column<br />
according to time programs of single<br />
fractions. If controlled by EuroChrom ®<br />
2000 Basic Edition V 1.65 or higher,<br />
EuroChrom ® 2000 for Windows, Preparative<br />
Version V 2.05 or ChromGate ®<br />
system software, fraction collection<br />
can be additionally performed due<br />
to signal levels or slope.<br />
Rack Configurations<br />
4 diverter plates with 48 positions,<br />
300 x 20 ml collectors<br />
120 x 50 ml collectors<br />
48 x 250 ml collectors<br />
Fraction Collection Time<br />
1 – 999 seconds or minutes<br />
Maximum Flow Rate<br />
200 ml/min<br />
Dimension and Weight<br />
420 x 480 x 550 mm (W x H x D),<br />
16.0 kg<br />
45
WellChrom<br />
<strong>HPLC</strong> Online Degasser<br />
Description<br />
Online degassers are suitable for the<br />
removal of dissolved air and other<br />
gases from the solvent. Otherwise,<br />
dissolved gases can degass inside the<br />
pump head during the suction phase.<br />
The flow precision may be reduced if<br />
a degasser is used. On the other hand,<br />
electrochemical and refractive index<br />
detectors produce a much better<br />
measuring signal due to less noise.<br />
Degassing Method<br />
Separate vacuum chambers with microporous<br />
teflon membranes.<br />
Degassing Efficiency<br />
≈ 0.4 ppm at 10 ml/min flow rate<br />
≈ 0.2 ppm at 1 ml/min flow rate<br />
Vacuum Monitoring<br />
Continuous CPU-based monitoring,<br />
± 2 Torr, digital display, error log<br />
Specials<br />
Output for error signal, exhaust vent<br />
connector, No-Ox connections<br />
Material<br />
Teflon, polyphenylene sulfide<br />
Dimensions and Weight<br />
80 x 145 x 285 mm (W x H x D),<br />
3.0 kg<br />
Important Note<br />
Eluents do not get into contact with<br />
metal parts (K-5002 and K-5004).<br />
This option is available for K-5020<br />
upon request.<br />
46<br />
WellChrom <strong>HPLC</strong> Online Degasser<br />
K-5002, K-5004, and K-5020<br />
Ordering Information<br />
Order No. For Analytical <strong>HPLC</strong> Quantity<br />
A2009 WellChrom <strong>HPLC</strong> Online Degasser K-5002, 2 channels 1<br />
A2015 WellChrom <strong>HPLC</strong> Online Degasser K-5004, 4 channels 1<br />
Order No. For Preparative <strong>HPLC</strong> Quantity<br />
A2014 WellChrom <strong>HPLC</strong> Online Degasser K-5020, 2 channels<br />
inert version available upon request 1
<strong>HPLC</strong> Mixing Chambers<br />
Dynamic Mixing Chamber, analytical version<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0285 Dynamic Mixing Chamber, stainless steel 1<br />
A0701 Set of spare parts, containing 4 sieves (stainless steel), 6 gaskets 1<br />
A0275 Dynamic Mixing Chamber, titanium 1<br />
A0365 Set of spare parts, containing 4 sieves (PTFE), 6 gaskets 1<br />
A1174 Dynamic Mixing Chamber, PEEK 1<br />
Dynamic Mixing Chamber, preparative version<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0581 Dynamic Mixing Chamber, stainless steel 1<br />
A2515 Optional for extending the mixing volume,<br />
1 intermediate volume element, 4.8 ml, 3 screws 6 x 60 mm 1<br />
A1051 Set of spare parts including 5 gaskets<br />
18.5 mm ID, 21.05 mm AD 1<br />
Description<br />
Suitable for work with high and low<br />
pressure gradient systems. The mixing<br />
chamber made out of titanium enables<br />
metal-free, biocompatible operation.<br />
The mixing chamber made out of PEEK<br />
can be used for biochromatographic<br />
purposes, but has a restricted pressure<br />
stability.<br />
Variable Chamber Volumes<br />
260 µl – ≈ 1,700 µl<br />
Biocompatibility<br />
If the appropriate type of mixing chamber<br />
is used, only titanium or PEEK parts<br />
come into contact with solvents.<br />
Pressure Stability for PEEK<br />
Maximum 120 bar<br />
Dimensions and Weight<br />
80 x 45 x 120 mm (W x H x D) or<br />
80 x 100 x 120 mm (W x H x D),<br />
1.5 kg<br />
Description<br />
Suitable for work with high and low<br />
pressure gradient systems. The mixing<br />
chamber made out of titanium enables<br />
metal-free, biocompatible operation.<br />
The mixing chamber made out of PEEK<br />
can be used for biochromatographic<br />
purposes, but has a restricted pressure<br />
stability.<br />
Chamber Volume<br />
5.9 ml<br />
Biocompatibility<br />
If the appropriate type of mixing chamber<br />
is used, only titanium or PEEK parts<br />
come into contact with solvents.<br />
Maximum Operating Pressure<br />
150 bar<br />
Dimensions and Weight<br />
80 x 130 x 125 mm (W x H x D),<br />
1.6 kg<br />
47
Thermostats<br />
and Flowmeter<br />
Description<br />
Suitable for a permanent monitoring<br />
of flow rates in <strong>HPLC</strong>.<br />
Measuring Range<br />
0.05 – 25 ml/min<br />
Accuracy<br />
±1%<br />
Weight<br />
0.5 kg<br />
Description<br />
The Column Box is ideal for use at<br />
room temperature. It prevents shortterm<br />
temperature fluctuations in chromatographic<br />
separations by covering<br />
<strong>HPLC</strong> columns in dimensions up to<br />
300 x 60 mm ID.<br />
Weight<br />
4.0 kg<br />
Description<br />
Suitable for column thermostatization<br />
above and below room temperature.<br />
Leak detection and a safety function<br />
are integrated. Operates with a Peltierelement<br />
capable of heating and cooling.<br />
Linear and non-linear temperature gradients<br />
can be programmed.<br />
Temperature Range<br />
5–85°C<br />
Temperature Stability and Accuracy<br />
± 0,1 °C, ± 0,2 °C<br />
Heating and Cooling Rate<br />
2 °C/min<br />
Power Supply<br />
100 – 245 V, 50/60 Hz, 100 W<br />
Dimensions<br />
Inside:<br />
90 x 100 x 400 mm (W x H x D)<br />
Outside:<br />
140 x 300 x 470 mm (W x H x D)<br />
48<br />
Flowmeter<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0916 Flowmeter, model 502500, with single-point calibration<br />
at 1 ml/min, with RS-232 interface 1<br />
Column Box<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0253 Column Box 1<br />
Column Thermostat Jetstream<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0527 Column Thermostat Jetstream, 5 – 85 °C,<br />
with 6-Port/2-Channel Injection Valve A1365, stainless steel 1<br />
A0585 Column Thermostat, 5 – 85 °C, injection valve included 1<br />
A1999 Column Thermostat, 5 – 85 °C,<br />
with 6-Port/2-Channel Injection Valve A1366, PEEK 1
<strong>HPLC</strong> <strong>Accessories</strong><br />
<strong>HPLC</strong> Standard Accessory Kit<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1071 <strong>HPLC</strong> Standard Accessory Kit (weight 0.8 kg) 1<br />
Consists of:<br />
Ferrules for Capillaries<br />
1/16”, AD stainless steel 50<br />
1/8”, AD stainless steel 4<br />
Sealing Rings for Capillaries<br />
1/16”, Polymer 100<br />
1/16”, PEEK 20<br />
1/8”, Polymer 5<br />
Bushings for Capillaries<br />
1/16”, short, UNF 10/32, wrench caliber 1/4”, stainless steel 10<br />
1/16”, long, UNF 10/32, wrench caliber 1/4”, stainless steel 10<br />
1/8”, long, M8 x 0,75, wrench caliber 10, stainless steel 3<br />
1/16”, short, UNF 10/32, knurled, polymer 5<br />
1/16”, short, UNF 10/32, with integrated sealing cone,<br />
knurled, polymer 5<br />
Polymer Caps<br />
for closing columns, connectors, and, flow cells<br />
UNF 10/32, knurled, polymer 10<br />
Low Dead Volume Coupling<br />
for 2 capillaries 1/16, stainless steel 5<br />
for 2 capillaries 1/16, polymer 5<br />
for capillaries 1/16” 1/8”, stainless steel 1<br />
T-Connector for 3 Capillaries<br />
1/16”, stainless steel 1<br />
1/16”, polymer 1<br />
Cross-Connector for 4 Capillaries<br />
1/16”, stainless steel 1<br />
1/16”, Polymer 1<br />
DYNASEAL connection system for capillaries<br />
1/16”, short 10<br />
1/16”, long 5<br />
1/8”, short 2<br />
Split-Grooved Clamping Ring for Capillaries<br />
1/16”, stainless steel 30<br />
1/8”, stainless steel 3<br />
Double-Cone Sealing Ring for Capillaries<br />
1/16”, polymer 20<br />
Open-End Wrenches<br />
Set of wrench caliber 1/4” and 5/16” 1<br />
Foreceps 1<br />
Description<br />
<strong>KNAUER</strong> offers a wide range of <strong>HPLC</strong><br />
fittings, connectors, ferrules, and more.<br />
These parts are compatible with commonly<br />
used injection and switching<br />
valves such as Rheodyne, Valco, or<br />
Swagelok. They are compatible for<br />
standard connections for both <strong>HPLC</strong><br />
columns and instruments.<br />
We strongly recommend our DYNASEAL<br />
connection system that is available in<br />
1/16” and 1/8” for analytical and<br />
preparative purposes.<br />
All parts are also available in PEEK.<br />
49
<strong>HPLC</strong> <strong>Accessories</strong><br />
Description<br />
This kit is recommended when ordering<br />
an analytical <strong>HPLC</strong> system for the first<br />
time.<br />
Weight<br />
0.5 kg<br />
Description<br />
This kit is recommended when ordering<br />
an analytical, biocompatible, and inert<br />
<strong>HPLC</strong> system for the first time.<br />
Weight<br />
0.2 kg<br />
50<br />
Start-Up Kit, analytical<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0910 Start-Up Kit, analytical 1<br />
Consists of:<br />
A0131 Capillary, 1/16” AD, 0.25 mm ID, stainless steel 3 m<br />
A0132 Capillary, 1/16” AD, 0.5 mm ID, stainless steel 3 m<br />
A0133 Capillary, 1/16” AD, 0.75 mm ID, stainless steel 3 m<br />
A0129 Set of precut capillaries, 1/16” 8<br />
A0137 Capillary cleaning set 1<br />
A0118 Low dead volume coupling of 2 capillaries, 1/16”, stainless steel 5<br />
A0112 Bushings for capillaries, 1/16”, stainless steel 10<br />
A0110 Ferrules for capillaries, 1/16” 30<br />
A0146 Caps, 1/16”, polymer 1<br />
A0120 T-connector for 3 capillaries, 1/16”, stainless steel 1<br />
A0152 PTFE tubing, 1/16”, 0.45 mm ID 3 m<br />
A0153 PTFE tubing, 2 mm x 1.4 mm ID 3 m<br />
A0108 Set of DYNASEAL connection system, short 2<br />
A0181 Set of DYNASEAL connection system, long 1<br />
A0726 Injection syringe, 100 µl 1<br />
A0809 Pliers for capillaries 1<br />
Start-Up Kit, biocompatible<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1039 Start-Up Kit, biocompatible 1<br />
Consists of:<br />
A0685 Capillary, PEEK, 1/16”, 0.25 mm ID 1.5 m<br />
A0691 Capillary, PEEK, 1/16”, 0.5 mm ID 1.5 m<br />
A0692 Capillary, PEEK, 1/16”, 0.75 mm ID 1.5 m<br />
A0137 Capillary cleaning set 1<br />
A0149 Low dead volume coupling of 2 capillaries, 1/16”, polymer 5<br />
A0585 Bushings for capillaries, 1/16”, short, PEEK 10<br />
A0139 Ferrules for capillaries, 1/16”, PEEK 30<br />
A0146 Caps, 1/16”, polymer 10<br />
A0150 T-connector for 3 capillaries, 1/16”, polymer 1<br />
A0152 PTFE tubing, 1/16”, 0.45 mm ID 3 m<br />
A0153 PTFE tubing, 2 mm x 1.4 mm ID 3 m<br />
A0108 Set of DYNASEAL connection system, short 2<br />
A0181 Set of DYNASEAL connection system, long 1<br />
A0726 Injection syringe, 100 µl 1<br />
A0569 Tube cutter 1
Start-Up Kit, preparative<br />
Ordering Information<br />
Order No. Article Quantity<br />
A1104 Start-Up Kit, preparative 1<br />
Consists of:<br />
A0639 Capillary, 1/8”, 1.6 mm ID 1.5 m<br />
A0640 Capillary, 1/8”, 2.2 mm ID 1.5 m<br />
A0134 Capillary, 1/16”, 1.0 mm ID 3 m<br />
A0873 PTFE tubing, 1/8”, 2 mm ID 3 m<br />
A0732 PTFE tubing, 1/8”, 1.5 mm ID 3 m<br />
A0154 PTFE tubing, 4 mm OD, 3 mm ID 3 m<br />
A0737 Capillary, 1/8”, PEEK, 1.6 mm ID 1.5 m<br />
A0478 Tefzel tubing, 1/8”, 1.6 mm ID 3 m<br />
A0644 DYNASEAL connection system for capillaries 1/8”, stainless steel 1<br />
A0830 Bushing for capillaries 1/8”, stainless steel 1<br />
A2511 T-connector for 3 capillaries 1/8”, short, stainless steel 1<br />
A2512 Low dead volume coupling for 2 capillaries 1/8”, stainless steel 5<br />
A2513 Low dead volume coupling for capillaries 1/16” 1/8”, stainless steel 1<br />
A0117 Low dead volume coupling for 2 capillaries 1/16”, stainless steel 1<br />
A0736 Set of DYNASEAL connection systems, 1/8”, long 1<br />
A0232 Sealing ring for capillaries 1/8”, polymer 10<br />
A0874 Ferrules for capillaries 1/8”, stainless steel 10<br />
A1239 Split-grooved clamping ring for capillaries 1/8” 4<br />
X0003 Set of two open-end wrenches, wrench caliber 1/4” and 5/16” 2<br />
X0030 Open-end wrench, wrench caliber 8/10” 2<br />
A0573 Luer-Lock syringe, glass, 10 ml 1<br />
A0734 Caps, 1/8”, polymer 10<br />
Filters for Sample Preparation<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0950 All glass vacuum unit 1<br />
A0951 PTFE membrane filter, 47 mm ∅, pore size 0.45 µm 100<br />
A0917 Membrane filter made out of regenerated cellulose,<br />
47 mm ∅, pore size 0.45 µm 100<br />
Description<br />
Recommended when ordering a preparative<br />
<strong>HPLC</strong> system for the first time.<br />
Weight<br />
0.9 kg<br />
Description<br />
Recommended for sample preparation.<br />
51
<strong>HPLC</strong> <strong>Accessories</strong><br />
Description<br />
<strong>KNAUER</strong>’s sample loops are suitable for<br />
all types of <strong>HPLC</strong> systems. Sample loops<br />
made out of PEEK will be delivered with<br />
our DYNASEAL connection system for<br />
capillaries 1/16”.<br />
Individual sizes available upon request.<br />
We recommend using the loop filling<br />
port if the injection syringe cannot be<br />
inserted into the injection port.<br />
Description<br />
Suitable for 1/16” and 1/8” connections.<br />
Useful for full and partial loop filling.<br />
The 11 ml and 45 ml sample loops<br />
consist of an empty stainless steel<br />
column with an internal piston.<br />
Low peak broadening. Applicable for<br />
flow rates exceeding 1 ml/min.<br />
Description<br />
Glass syringes are suitable for all standard<br />
<strong>HPLC</strong> injection valves.<br />
52<br />
Sample Loops, analytical<br />
Ordering Information<br />
Sample Loops, stainless steel<br />
Order No. Sample Volume Quantity<br />
A0331 5 µl 1<br />
A0561 10 µl 1<br />
A0562 20 µl 1<br />
A0836 30 µl 1<br />
A0563 50 µl 1<br />
A0837 75 µl 1<br />
A0564 100 µl 1<br />
A0565 200 µl 1<br />
A0566 500 µl 1<br />
A0567 1,000 µl 1<br />
A0568 2,000 µl 1<br />
Sample Loops, preparative<br />
Ordering Information<br />
Order No. Sample Loops Quantity<br />
A1159 1/8’’, volume up to 11 ml 1<br />
A1160 1/8’’, volume up to 45 ml 1<br />
A1054 1/16”, volume up to 11 ml 1<br />
A1055 1/16”, volume up to 45 ml 1<br />
A1043 1/8”, volume up to 1 ml, stainless steel 1<br />
A1110 1/8”, volume up to 1 ml, PEEK 1<br />
A1044 1/8”, volume up to 2 ml, stainless steel 1<br />
A1111 1/8”, volume up to 2 ml, PEEK 1<br />
Injection Syringes<br />
Ordering Information<br />
Order No. Sample Volume Quantity<br />
A0723 10 µl 1<br />
A0724 25 µl 1<br />
A0725 50 µl 1<br />
A0726 100 µl 1<br />
Sample Loops, PEEK<br />
Order No. Sample Volume Quantity<br />
A1058 10 µl 1<br />
A1059 20 µl 1<br />
A1060 50 µl 1<br />
A1061 200 µl 1<br />
A1057 500 µl 1<br />
Loop Filling Port<br />
Order No. Article Quantity<br />
A0555 Loop filling port 1<br />
Order No. Sample Volume Quantity<br />
A0727 250 µl 1<br />
A0728 500 µl 1<br />
A0729 1,000 µl 1<br />
A0730 2,500 µl 1
Capillaries, stainless steel<br />
Ordering Information<br />
Order No. OD (inch) ID (mm) Length (cm) Quantity<br />
A0130 1/16” 0.10 300 1<br />
A0131 1/16” 0.25 300 1<br />
A0132 1/16” 0.50 300 1<br />
A0133 1/16” 0.70 300 1<br />
A0134 1/16” 1.00 300 1<br />
A0123 1/16” 0.10 10 10<br />
A0124 1/16” 0.10 20 10<br />
A0125 1/16” 0.10 30 10<br />
A0126 1/16” 0.25 10 10<br />
A0127 1/16” 0.25 20 10<br />
A0128 1/16” 0.25 30 10<br />
A0639 1/8” 1.60 150 1<br />
A0640 1/8” 2.20 150 1<br />
Tools for Capillaries, stainless steel<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0137 Capillary cleaning set 1<br />
A0809 Pliers for capillaries 1<br />
Capillaries, PEEK<br />
Ordering Information<br />
Order No. OD (inch) ID (mm) Length (cm) Quantity<br />
A0685 1/16” 0.25 150 1<br />
A0691 1/16” 0.50 150 1<br />
A0692 1/16” 0.70 150 1<br />
A0737 1/8” 1.60 150 1<br />
Description<br />
Resistant capillaries made out of stainless<br />
steel for all <strong>HPLC</strong> connections.<br />
Description<br />
Tools to keep your capillaries in »shape«.<br />
Description<br />
Suitable for biochromatography.<br />
Metal-free PEEK capillaries consist of<br />
polyether-ether-ketone that show a<br />
pressure stability of up to 400 bar<br />
depending on the inner diameter.<br />
Don’t forget to take advantage of our<br />
DYNASEAL connection system when<br />
working with PEEK capillaries.<br />
53
<strong>HPLC</strong> <strong>Accessories</strong><br />
Description<br />
Tefzel tubings consist of perfluorinated<br />
hydrocarbons. They are flexible, inert,<br />
and gas tight. Pressure stability reaches<br />
up to 200 bar depending on the inner<br />
diameter.<br />
Description<br />
PTFE tubings consist of perfluorinated<br />
hydrocarbons. They are flexible, biocompatible,<br />
and gas tight.<br />
Description<br />
Cuts all flexible plastic tubes, particularly<br />
suitable for capillaries made out of<br />
PEEK.<br />
54<br />
Tefzel Tubings<br />
Ordering Information<br />
Order No. OD (inch) ID (mm) Length (cm) Quantity<br />
A0182 1/16” 0.25 – 0.30 300 1<br />
A0183 1/16” 0.70 300 1<br />
A0478 1/8” 1.60 300 1<br />
PTFE Tubings<br />
Ordering Information<br />
Order No. OD (inch) ID (mm) Length (cm) Quantity<br />
A0152 1.6 (1/16”) 0.45 300 1<br />
A0153 2 1.45 300 1<br />
A0732 3.2 (1/8”) 1.5 300 1<br />
A0873 3.2 (1/8”) 2.00 300 1<br />
A0154 4 3.00 300 1<br />
Tube Cutter, Ferrules, and Sealing Rings<br />
Ordering Information<br />
Order No. Tube Cutter Quantity<br />
A0569 Tube cutter 1<br />
Order No. Ferrules for capillaries Quantity<br />
A0110 1/16”, stainless steel 30<br />
A0111 1/16”, stainless steel 100<br />
A0874 1/8”, stainless steel 10<br />
Order No. Sealing Rings for capillaries Quantity<br />
A0139 1/16”, polymer 30<br />
A0140 1/16”, polymer 100<br />
A0232 1/8”, polymer 10<br />
A1062 1/16”, PEEK 10<br />
A1063 1/8”, PEEK 10
Couplings, T-Connectors, and Cross Connectors<br />
Ordering Information<br />
Low Dead Volume Couplings, stainless steel<br />
Order No. for connecting: Quantity<br />
A0117 2 capillaries 1/16” 1<br />
A0118 2 capillaries 1/16” 5<br />
A0119 2 capillaries 1/16” 25<br />
A2512 2 capillaries 1/8” 5<br />
A2513 2 capillaries 1/16” 1/8” 1<br />
Low Dead Volume Couplings, polymer<br />
Order No. for connecting: Quantity<br />
A0148 2 capillaries, 1/16” 1<br />
A0149 2 capillaries, 1/16” 5<br />
T-Connector, stainless steel<br />
Order No. for connecting: Quantity<br />
A0120 3 capillaries 1/16” 1<br />
A2511 3 capillaries 1/8” 1<br />
T-Connector, polymer<br />
Order No. for connecting: Quantity<br />
A0150 3 capillaries 1/16” 1<br />
Cross-Connector, stainless steel<br />
Order No. for connecting: Quantity<br />
A0121 4 capillaries 1/16” 1<br />
A1096 4 capillaries 1/8” 1<br />
Cross-Connector, stainless steel<br />
Order No. for connecting: Quantity<br />
A2514 3 capillaries of 1/16” and 1 capillary of 1/8” 1<br />
Cross-Connector, polymer<br />
Order No. for connecting: Quantity<br />
A0151 4 capillaries of 1/16” 1<br />
Description<br />
All parts made out of polymer consist<br />
of PETP (Arnite) if not mentioned<br />
otherwise.<br />
55
<strong>HPLC</strong> <strong>Accessories</strong><br />
Description<br />
All bushings have inch threading (UNF)<br />
and are available with short and long<br />
heads. The long head bushings are<br />
recommended when tight connections<br />
are required.<br />
Ferrules are slipped over the capillary,<br />
deformed during tightening, and cannot<br />
be removed afterwards. Thus, the<br />
connection is sealed.<br />
Description<br />
56<br />
Bushings and Caps (Blind Fittings)<br />
Ordering Information<br />
Order No. Bushings for Capillaries Quantity<br />
A0112 1/16’’, short, UNF 10/32, SW 1/4’’, stainless steel 10<br />
A0113 1/16’’, short, UNF 10/32, SW 1/4’’, stainless steel 25<br />
A0115 1/16’’, long, UNF 10/32, SW 1/4’’, stainless steel 3<br />
A0116 1/16’’, long, UNF 10/32, SW 1/4’’, stainless steel 10<br />
A0830 1/8’’, M8 x 1, SW 10, stainless steel 10<br />
A0141 1/16’’, short, UNF 10/32, knurled, polymer 10<br />
A0142 1/16’’, short, UNF 10/32, knurled, polymer 30<br />
A0143 1/16’’, short, UNF 10/32, knurled, polymer 100<br />
A0144 1/16’’, long, UNF 10/32, knurled, polymer 10<br />
A0145 1/16’’, short, UNF 10/32, with double-cone sealing,<br />
knurled, polymer 10<br />
A0733 1/8’’, short, UNF 10/32, M8 x 1, with sealing ring,<br />
knurled, polymer 10<br />
A0584 1/16’’, UNF 10/32, PEEK 10<br />
Order No. Caps (blind fittings) Quantity<br />
A0146 1/16’’, short, UNF 10/32, polymer 10<br />
A0147 1/16’’, short, UNF 10/32, polymer 30<br />
A0734 1/8’’, M8 x 1, polymer 10<br />
A0582 1/16’’, short, UNF 10/32, knurled, polymer 10<br />
DYNASEAL Connection System<br />
Ordering Information<br />
Order No. Bushings for Capillaries Quantity<br />
A1021 1/16”, short 10<br />
A1064 1/16”, long 5<br />
A1067 1/8”, short 4<br />
A0735 1/8”, long 4<br />
Order No. Split-Grooved Clamping Rings Quantity<br />
A0484 1/16” 4<br />
A1239 1/8” 4<br />
Order No. Sealing Rings Quantity<br />
A0139 1/16”, polymer 30<br />
A0140 1/16”, polymer 100<br />
A1062 1/16”, PEEK 10<br />
A0232 1/8”, polymer 10<br />
A1063 1/8”, PEEK 10<br />
Order No. Double-Cone Sealings Quantity<br />
A1022 1/16”, polymer 10<br />
A1070 1/16”, PEEK 10
DYNASEAL Connection System<br />
Ordering Information – System –<br />
Order No. Article Quantity<br />
A0108 1/16”<br />
Short head bushings 4<br />
Split-grooved clamping rings 4<br />
Sealing rings 8<br />
A0181 1/16”<br />
Long head bushings 3<br />
Split-grooved clamping rings 3<br />
Sealing rings 4<br />
A1020 1/16”<br />
Short head bushings 10<br />
Double-cone sealings 10<br />
A1069 1/16”<br />
Long head bushings 5<br />
Double-cone sealings 5<br />
A0736 1/8”, M8 x 0.1<br />
Long head bushings 4<br />
Split-grooved clamping rings 4<br />
Sealing rings 8<br />
A0644 1/8”, M8 x 1<br />
Short head bushings 4<br />
Split-grooved clamping rings 4<br />
Sealing rings 8<br />
Description<br />
The DYNASEAL connection system<br />
connects capillaries made out of stainless<br />
steel and PEEK, as well as PTFE and<br />
Tefzel tubings with saftey and low dead<br />
volumes. It allows maintenance-free<br />
operation and provides a long life.<br />
Suitable for UNF-threads of type 10/32.<br />
The system consists of a bushing, a splitgrooved<br />
clamping ring, and a polymer<br />
sealing. The split-grooved clamping<br />
ring and polymer sealing are slipped<br />
over the capillary »back to back«, while<br />
the bushing tightens all parts. Thus,<br />
leak-free operation is made possible.<br />
DYNASEAL connections are pressure<br />
stable up to 450 bar.<br />
DYNASEAL can be optionally used with<br />
double-cone sealings made out of PEEK<br />
or stainless steel. In this case, pressure<br />
stability is accordingly reduced to 150 bar<br />
(PEEK) and 350 bar (polymer).<br />
DYNASEAL is adaptable in new conditions<br />
such as variable bore depths or<br />
detector connections because it is<br />
mobile and reuseable.<br />
Important Note<br />
The DYNASEAL connection system<br />
is available for capillaries with 1/16”<br />
(analytical <strong>HPLC</strong>) and 1/8” (preparative<br />
<strong>HPLC</strong>).<br />
In general, to connect <strong>KNAUER</strong>, Rheodyne,<br />
or other injection systems; the<br />
long head bushings can be delivered<br />
upon request.<br />
57
<strong>HPLC</strong> <strong>Accessories</strong><br />
Description<br />
The Column Inlet Filter can be installed<br />
between the injection system and the<br />
column. Column inlet filters protect<br />
the column from particle contamination<br />
caused by sealing material (i. e. pump<br />
pistons). We recommend adding a filter<br />
after the column. This prevents contamination<br />
of the detector cell. Inlet filters<br />
are available in stainless steel and<br />
PEEK.<br />
Description<br />
Ready-to-use filtration units for ultrapurification<br />
of small sample volumes.<br />
The chemically resistant polypropylene<br />
housing has been designed to achieve<br />
a maximum sample yield with a high<br />
retention time. Backflow/overflow of<br />
the filtrate is effectively avoided with<br />
elongated Spike and Luer outlets.<br />
58<br />
Column Inlet Filters<br />
Ordering Information<br />
Order No. Article Quantity<br />
A0109 Column Inlet Filter, stainless steel 1<br />
A0015 Set of spare parts for A1090, includes:<br />
6 sieves (3 µm), 6 sieves (7 µm), 12 glass filters, 6 PTFE gaskets 1<br />
A0138 Set of open-end wrenches, wrench caliber 1/4” and 5/16” 2<br />
A0573 Luer-Lock glass syringe, 10 µl 1<br />
A0653 Luer-Lock glass syringe, 20 µl 1<br />
Syringe Filtration Units<br />
Ordering Information<br />
Polypropylene-reinforced PTFE (hydrophobe)<br />
Pore Size Dead Volume � Area<br />
Order No. Article µm µl mm cm 2 Quantity<br />
A1258 Minisart SRP4 0.45 1 4 0.10 50<br />
A1259 Minisart SRP4 0.45 1 4 0.10 500<br />
A0958 Minisart SRP15 0.45 10 15 1.70 50<br />
A1264 Minisart SRP15 0.45 10 15 1.70 500<br />
A0953 Minisart SRP15 0.20 10 15 1.70 50<br />
A0954 Minisart SRP15 0.20 10 15 1.70 500<br />
A0956 Minisart SRP25 0.20 100 25 4.80 50<br />
A0957 Minisart SRP25 0.45 100 25 4.80 50<br />
A1262 Minisart SRP25 0.45 100 25 4.80 500<br />
Cellulose (hydrophile)<br />
Pore Size Dead Volume � Area<br />
Order No. Article µm µl mm cm2 Quantity<br />
A1260 Minisart RC4 0.45 5 4 0.07 50<br />
A1261 Minisart RC4 0.45 5 4 0.07 500<br />
A1265 Minisart RC15 0.45 10 15 1.70 50<br />
A0960 Minisart RC15 0.45 10 15 1.70 500<br />
A0961 Minisart RC25 0.20 150 25 4.80 500<br />
A1263 Minisart RC25 0.45 150 25 4.80 50<br />
A0962 Minisart RC25 0.45 150 25 4.80 500
Terms of Delivery<br />
Terms of Delivery<br />
Our general delivery and payment conditions are valid as follows:<br />
Our deliveries follow ex works. As soon as the merchandise leaves our factory accidental<br />
loss, damage, destruction, or deterioration is at the buyer’s own risk. The guarantee<br />
is valid for one year with the exception of cullet, expendable items such as lamps,<br />
columns, seals, valves, and damage due to clogging or jamming. This guarantee<br />
strictly applies to osmometers and devices used in liquid chromatography.<br />
The delivered merchandise will remain our property until payment has been made,<br />
received in full, and all claims have been met. We reserve the right for any modifications<br />
in technical advancement.<br />
Address<br />
Wissenschaftliche Gerätebau<br />
Dr. Ing. Herbert Knauer GmbH<br />
Hegauer Weg 38<br />
D-14163 Berlin (Zehlendorf)<br />
Germany<br />
Telephone: +49 (0)30 80 97 27- 0<br />
Fax: +49 (0)30 8 01 50 10<br />
E-mail: info@knauer.net<br />
Internet: www.knauer.net<br />
Bank Information<br />
� Commerzbank AG, Berlin<br />
Bank code number: 100 400 00<br />
Account number: 65 02 900<br />
� Berliner Bank AG, Berlin<br />
Bank code number 100 200 00<br />
Account number 99 80 22 52 01<br />
59
Conditions of Sale<br />
Conditions of Sale<br />
1. Definition of Terms<br />
The following terms and conditions apply to every order received<br />
by us. This is especially applicable in case of contradictory buying<br />
conditions of sale. Exceptions are only valid when confirmed<br />
by us in writing.<br />
Purchase orders are only binding if confirmed by us in writing.<br />
2. Payment<br />
Domestic deliveries are payable, net, within 30 days of invoice<br />
date or in advance. Deductions are not allowed.<br />
Foreign deliveries must be paid by irrevocable letter of credit or<br />
in advance. All bank and transfer fees must be paid by the<br />
customer. The maturity date passed the customer is in default<br />
even without an additional reminder. Upon delay interest of five<br />
percent above the German Federal Bank discount rate will be<br />
added to the purchase price, if we do not prove a higher damage<br />
or the customer proves a lower damage.<br />
Payments are due irrespective of an eventual notice of defect,<br />
except such defects which are evidently justified.<br />
3. Delivery<br />
Delivery dates are not binding. Delay in delivery requires a<br />
written reminder and an adequate additional period of grace set<br />
by the customer, after which customer may rescind the contract.<br />
Claims for damages of any kind are excluded.<br />
4. Claims<br />
Condition for any warranty claim is the immediate inspection of<br />
the goods upon delivery, complaint towards and damage assessment<br />
together with the carrier, and an immediate written complaint<br />
to us. The complaint must be made within three workdays<br />
in case of visible defects or losses.<br />
5. Risk Liability<br />
Delivery is made at the customer’s own risk. As soon as the<br />
goods leave our plant the risk of accidental loss, destruction or<br />
deterioration passes to the customer.<br />
6. Guarantee<br />
Our warranty for osmometers and liquid chromatography instruments<br />
is limited to one year, excluding glass breakage,<br />
damages due to stoppage and consumable materials such as<br />
membranes, light bulbs, columns, bushings, gaskets and valves.<br />
Our warranty is limited to repair or replacement at no costs except<br />
if we have guaranteed certain characteristics explicitly and<br />
in writing. Transportation costs are borne by the customer. In<br />
case a repair or eventual replacement is also defective the customer<br />
may rescind the contract or may claim a reduction in<br />
price.<br />
Warranty claims are barred if the customer denies us repair or<br />
replacement of the defective product within a reasonable<br />
period of time not exceeding three months counting from the<br />
date of the written complaint.<br />
We are not liable for defects originating after the passing of the<br />
risk, for defects originating in improper or careless handling of<br />
the goods by the customer, or for defects caused by inappropriate<br />
or not permitted operating supplies.<br />
We are liable for monetary compensation only for damages<br />
caused by the absence of expressly and in writing warranted<br />
characteristics, for damages caused by us or persons employed<br />
60<br />
by us, either intentionally or – in case of a breach of condition of<br />
the contract – negligently, otherwise only in case of gross negligence.<br />
Our liability for damages is limited to direct damages except in<br />
case a specific warranty was given to cover the customer also<br />
against indirect damages. We are not liable for advice given.<br />
Liability according to the stipulations of the German Law on<br />
Product Liability remains unaffected.<br />
7. Property Rights<br />
The ownership of the goods shall remain with us until payment<br />
in full for all our claims resulting from our business relation is<br />
received. In case of improper treatment of the goods or in case<br />
of default we may demand the return of the delivered goods.<br />
Towards merchants this demand entails resignation of the contract<br />
only if we declare it explicitly.<br />
Resalers are allowed to sell the goods to third parties in due<br />
course of the business. The customer herewith assigns his resale<br />
claims against third parties to us.<br />
8. Export<br />
<strong>Instruments</strong> and products delivered by us may not be exported<br />
to a country other than of the customer’s headquarters without<br />
our prior written permission.<br />
9. Place of Settlement and Court of Jurisdiction<br />
If the customer is a merchant the place of performance is Berlin.<br />
Proper venue for all claims is the competent local court at our<br />
principal place of business. We reserve the right to sue the<br />
customer at his principal place of business.<br />
This agreement shall be governed by the laws of the Federal<br />
Republic of Germany excluding the UN-Convention on the<br />
International Sale of Goods (CISG).
Please ask for our<br />
Flyers for individual Modules ...<br />
The WellChrom Series<br />
of Analytical <strong>HPLC</strong> Pumps<br />
Wissenschaftliche Gerätebau<br />
Dr. Ing. Herbert Knauer GmbH<br />
Hegauer Weg 38<br />
D-14163 Berlin, Germany<br />
Telephone: +49 (0)30/80 9727- 0<br />
Telefax: +49 (0)30/8 01 50 10<br />
E-Mail: info@knauer.net<br />
Internet: www.knauer.net<br />
Photometric<br />
Detection Systems<br />
The WellChrom Series<br />
of Autosamplers Preparative Pump K-1800<br />
<strong>KNAUER</strong> systems are manufactured based on the<br />
latest technology and over 40 years of experience.<br />
Our product and sales managers are specialized in<br />
configuring and / or adjusting all <strong>KNAUER</strong> systems.<br />
Dealers now located in over 50 countries!<br />
<strong>HPLC</strong> Autosampler K-3800<br />
Our customers are, therefore, equipped with excellent<br />
products and services. With expertise and<br />
patience we are committed to assisting your every<br />
need.<br />
Your local dealer:<br />
<strong>KNAUER</strong> ChromGate ®<br />
Chromatographic System<br />
Vapour Pressure<br />
Osmometer K-7000 <strong>HPLC</strong> Columns<br />
KN 08/01/06.2001/8.5 Weselmann Berlin