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Studies of vegetative propagation of the lychee (Litchi chinensis ...

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210 W. S. Abutiate & N. Y. Nakasone (1972) Ghana Jnl agric. Sci. S, 201-211<br />

Sitton (1929) and Swarbrick (1926). In this study, Buck, G. J. C1?53).The histological development o~<strong>the</strong><br />

<strong>the</strong> secondary cambial derivatives were observed to bud graft union In roses. Proc. Am. Soc. hort. Set. 62,<br />

'b call fi . b th '" 497-502.<br />

contn ue to us ormatIOn ut e partICIpatIOn<br />

<strong>of</strong> <strong>the</strong> ray cells in <strong>the</strong> healing process could not be<br />

clearly established. Crafts (1934) and Bradford &<br />

Sitton (1929) fur<strong>the</strong>r observed that <strong>the</strong> vascular<br />

cambium also contributed cells to <strong>the</strong> graft union<br />

but in this study <strong>the</strong>re was no conclusive evidence<br />

to establish <strong>the</strong> cambium as directly contributing to<br />

callus formation.<br />

As observed also by Mendel (1936) and Saas<br />

(1933) <strong>the</strong> pith did not contribute callus in <strong>the</strong><br />

healing process <strong>of</strong> <strong>the</strong> <strong>lychee</strong> grafts. The results<br />

<strong>of</strong> <strong>the</strong> side-wedge grafts also corroborated <strong>the</strong><br />

findings <strong>of</strong> Mendel (1936) that both <strong>the</strong> stock and<br />

scion may contribute callus in <strong>the</strong> healing process.<br />

From <strong>the</strong> growth behaviour <strong>of</strong> <strong>the</strong> <strong>lychee</strong>, Venning<br />

(1949) postulated that successful unions may<br />

depend upon <strong>the</strong> chances <strong>of</strong> any two active cambial<br />

areas being in juxtaposition. Since <strong>the</strong>re are no<br />

external indicators to cambial activity in this stem,<br />

success <strong>of</strong> union will undoubtedly depend upon<br />

<strong>the</strong> chances <strong>of</strong> two active cambial areas being in<br />

contact.<br />

The results <strong>of</strong> this study have indicated that<br />

successful grafting may depend upon <strong>the</strong> interplay<br />

between a number <strong>of</strong> factors including cambial<br />

activity in <strong>the</strong> two symbionts, <strong>the</strong> level <strong>of</strong> carbohydrates<br />

in <strong>the</strong> stems and cultivar characteristics.<br />

Both rooting <strong>of</strong> suitable stem cuttings with <strong>the</strong><br />

aid <strong>of</strong> hormones and side-grafting <strong>of</strong> old stocks<br />

using prepared scions may be used only to a<br />

limited extent. For large-scale <strong>propagation</strong>, however,<br />

young seedlings are preferred since a higher<br />

percentage <strong>of</strong> successful unions may be obtained.<br />

Recent work by Kadman & Slor in Israel has<br />

clearly established this fact.<br />

REFERENCES<br />

Artschwager, E. (1951) Anatomical studies <strong>of</strong> graft<br />

unions between guayule and sunflower. Tech. Bull.<br />

U.S. Dep. Agric. 1040, 16-17; 23-26.<br />

Bradford, F. C. & Sitton, B. G. (1929) Defective graft<br />

unions in <strong>the</strong> apple and <strong>the</strong> pear. Tech. Bull. Mich. St.<br />

Univ. agric. Exp. Stn 99,1-106.<br />

Brandon, Dorothy (1939) Seasonal variations <strong>of</strong> starch<br />

content in <strong>the</strong> genus Rosa and <strong>the</strong>ir relation to <strong>propagation</strong><br />

by stem cuttings.J. hort. Sci. 17,233-253.<br />

Carlson, M. C. (1929)Microchemical studies <strong>of</strong> rooting<br />

and non-rooting rose cuttings. Contr. Boyce Thompson<br />

Inst. Pl. Res. 1, 529-545.<br />

Chandler, W. H. (1958) Evergreen orchards, 2nd ed.<br />

Philadelphia: Lea & Febiger.<br />

Cooper, W. C. & Knowlton, K. R. (1939)The effect <strong>of</strong><br />

syn<strong>the</strong>tic growth substances on <strong>the</strong> rooting <strong>of</strong> subtropical<br />

fruit plants. Proc. Am. Soc. hort. Sci. 37,<br />

1093-1098.<br />

Crafts, A. S. (1934) Phloem anatomy in two species <strong>of</strong><br />

Nicotiana with notes on <strong>the</strong> interspecific graft union.<br />

Bot. Gaz. 94, 597-608.<br />

Doran, W. L. (1941) The <strong>propagation</strong> <strong>of</strong> some trees<br />

and shrubs by cuttings. Bull. Mass. agric. Exp. Stn<br />

382,56.<br />

Fahmy, Ibrahim (1952) Grafting studies on Macadamia<br />

and Sapodilla in relation to carbohydrate, using<br />

pre-girdled scions. Proc. Am. Soc. hort. Sci. 65,<br />

190-192.<br />

Horwitz, William (ed.) (1960) Official methods <strong>of</strong><br />

analysis <strong>of</strong> <strong>the</strong> Association <strong>of</strong> Official Agricultural<br />

Chemists, 9th ed., p. 832. Washington 4 D.C.:<br />

Benjamin Franklin Station.<br />

Jones, W. W. & Beaumong, J. H. (1937) Carbohydrate<br />

accumulation in relation to <strong>vegetative</strong> <strong>propagation</strong> <strong>of</strong><br />

<strong>the</strong> litchi (<strong>Litchi</strong> <strong>chinensis</strong>). Science, 86, 313.<br />

Juliano, Jose B. (1941) Callus development in graft<br />

unions. Philipp. J. Sci. 75, 245-251.<br />

Kadman, A. & Slor, E. Experiments with <strong>propagation</strong><br />

<strong>of</strong> <strong>the</strong> litchi (<strong>Litchi</strong> <strong>chinensis</strong>) in Israel. Indian J. Hort.<br />

(In press.)<br />

Kost<strong>of</strong>f, Dontcho (1938) <strong>Studies</strong> on callus tissue. Am.<br />

J. Bot. 15, 576.<br />

Kruas, E. J. & Kraybill, H. R. (1918) Vegetation and<br />

reproduction with special reference to <strong>the</strong> tomato.<br />

Bull. Ore. agric. Coil. 149, 1-10.<br />

Mendel, Kurt (1936) The anatomy and histology <strong>of</strong> <strong>the</strong><br />

bud union in citrus. Palest. J. Bot. hort. Sci. 1, 13-46.<br />

Mergen, Francois (1954) Anatomical study <strong>of</strong> slash<br />

pine graft union. Q.Jl Pla Acad. Sci. 17,238-245.<br />

Mollart, D. L. (1954) The control <strong>of</strong> cambial activity in<br />

Rubinia pseudacadia. (M.Sc. <strong>the</strong>sis.) Manchester:<br />

University <strong>of</strong> Manchester.<br />

Murneek, A. E. (1941) Relative carbohydrate and<br />

nitrogen concentration in new tissue produced on<br />

ringed branches. Proc. Am. Soc. hort. Sci. 38, 133-136.

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