Tetralogy of Fallot and Alterations in Vascular Endothelial Growth ...
Tetralogy of Fallot and Alterations in Vascular Endothelial Growth ...
Tetralogy of Fallot and Alterations in Vascular Endothelial Growth ...
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Ms # CIRCRESAHA/2006/140772-onl<strong>in</strong>e supplement/R2<br />
Figure legends<br />
Figure S1. Characterisation <strong>of</strong> the anti-Notch1 <strong>and</strong> anti-Jagged2 antibodies us<strong>in</strong>g<br />
compet<strong>in</strong>g peptides. The sta<strong>in</strong><strong>in</strong>gs performed are <strong>in</strong>dicated <strong>in</strong> the upper right corner. All<br />
sta<strong>in</strong><strong>in</strong>gs have been performed on a Vegf+/120 mouse embryo <strong>of</strong> E13.5. The sta<strong>in</strong><strong>in</strong>g is<br />
performed accord<strong>in</strong>g to the protocols described <strong>in</strong> this manuscript. Figure a <strong>and</strong> b are<br />
the positive controls whilst figure c <strong>and</strong> d show sections <strong>in</strong> which the primary antibody<br />
has been omitted. Figure e <strong>and</strong> f show sections <strong>in</strong>cubated with primary antibody<br />
pre<strong>in</strong>cubated with 75x compet<strong>in</strong>g peptide. Scale bar – 200 µM.<br />
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