Role of Intestinal Microbiota in Ulcerative Colitis
Role of Intestinal Microbiota in Ulcerative Colitis
Role of Intestinal Microbiota in Ulcerative Colitis
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difference from the lum<strong>in</strong>al microbiota, it is crucial to apply an <strong>in</strong> vitro model that accounts for the<br />
study <strong>of</strong> the mucosal microbiota.<br />
We have demonstrated that the ability to colonize the mucus was significantly lower for<br />
bifidobacteria and lactobacilli orig<strong>in</strong>at<strong>in</strong>g from UC patients when compared to those derived from<br />
healthy subjects (Table 3). In consistence with our observations, lower levels <strong>of</strong> bifidobacteria <strong>in</strong><br />
the mucus layer <strong>of</strong> biopsy specimens from UC patients have previously been described [39,43].<br />
Bifidobacteria and lactobacilli are believed to play an important role <strong>in</strong> promot<strong>in</strong>g <strong>in</strong>test<strong>in</strong>al<br />
health, due to their ability to <strong>in</strong>hibit colonization <strong>of</strong> pathogenic bacteria by lower<strong>in</strong>g <strong>of</strong> colonic pH<br />
[15], produce antimicrobial compounds [34,54], and compete for adhesion sites [7,9,35,54].<br />
Additionally, they have shown to stimulate immune regulatory responses [21,66]. Hence, a<br />
depletion <strong>of</strong> bifidobacteria and lactobacilli <strong>in</strong> UC patients could have a consequence for colonic<br />
health and favor <strong>in</strong>flammation. Stimulation <strong>of</strong> these groups through pre‐ or probiotics could be an<br />
approach <strong>in</strong> prevention <strong>of</strong> flare ups <strong>in</strong> UC.<br />
In previous literature, the glycosylation patterns <strong>in</strong> UC patients <strong>in</strong> relapse has shown to differ<br />
compared to control subjects and UC patients <strong>in</strong> remission, however, the aberrant pr<strong>of</strong>ile is<br />
reversible upon remission [33] and the microbiota has shown to be altered <strong>in</strong> UC patients <strong>in</strong><br />
relapse compared to controls but only to some extent <strong>in</strong> UC patients <strong>in</strong> remission [57,58,64]. It is<br />
not well understood how changes <strong>in</strong> mucus composition can affect adhesion and colonization <strong>of</strong><br />
gut microorganisms, however, a changed mucus niche <strong>in</strong> UC patients <strong>in</strong> relapse may select a<br />
different microbiota community and upon remission the level may not be reversed for all bacteria,<br />
even though the mucus structure is back to “normal” [33].<br />
Species or stra<strong>in</strong> specific mucus adhesion promot<strong>in</strong>g prote<strong>in</strong>s have been found <strong>in</strong> several<br />
bifidobacteria and lactobacilli. This <strong>in</strong>cludes among others fimbriae (L. rhamnosus GG, L. Johnsonii<br />
NCC533, B. animalis subsp. lactis, B. bifidum)[17,24,50], Msa, mannose‐specific adhes<strong>in</strong> prote<strong>in</strong> (L.<br />
plantarum) [49], MucBP doma<strong>in</strong> conta<strong>in</strong><strong>in</strong>g prote<strong>in</strong>s (lactic acid bacteria) [26], and adhesion‐like<br />
factor EF‐Tu (L. plantarum, B. animalis subsp. lactis) [17,63]. The expression <strong>of</strong> adhesion<br />
molecules may be changed <strong>in</strong> the lactic acid bacteria from UC patients, hence their <strong>in</strong>ability to<br />
colonize the mucus <strong>in</strong> vitro, however, to our knowledge, no species <strong>of</strong> bifidobacteria or lactobacilli<br />
have be isolated from UC patients to identify adhesion molecules.<br />
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