Role of Intestinal Microbiota in Ulcerative Colitis
Role of Intestinal Microbiota in Ulcerative Colitis Role of Intestinal Microbiota in Ulcerative Colitis
Introduction Methodology part The aim of this study was to examine the ability of fecal microbiota derived from UC patients in either remission or relapse, and healthy subjects to colonize an artificial mucus layer using a dynamic in vitro gut model. Flow diagram Paper 2 The author, Louise K. Vigsnæs, participated in the design of the study, conducted the experiments, performed the data analyzes and drafted the manuscript. The manuscript is still in a preliminary state. The following analyzes and data treatments will be added in the final version: • Growth curves for sixteen different bacterial taxa (see flow diagram): Samples were taken out at 0, 18, 26 and 42 hours from the lumen. Louise K. Vigsnæs is conducting these analyzes. • SCFA analysis for acetate, butyrate and propionate: Samples were taken out at 0, 1.5, 3, 5, 18, 20, 22, 23.5, 25, and 42 hours. Louise K. Vigsnæs and Pieter Van den Abbeele are conducting these analyzes. • Mass spectrometry (MS) on samples from the lumen and mucus (42 hours). Karolina Sulek is conducting the MS. 70
Fecal lactobacilli and bifidobacteria from ulcerative colitis patients display reduced ability to colonize mucus in the MSHIME. Louise Kristine Vigsnaes 1* , Pieter van den Abbeele 2 , Tom van de Wiele 2 and Tine Rask Licht 1 . 1 Department of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860 Søborg, Denmark. 2 Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B‐9000 Gent, Belgium *Corresponding author: Tel.: +45 35 88 70 24; Fax: +45 35 88 70 28; E‐mail: lokv@food.dtu.dk Key word: Ulcerative colitis, mucus colonization, qPCR, bifidobacteria, lactobacilli, butyrate‐ producing bacteria, dynamic gut model. 1
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Fecal lactobacilli and bifidobacteria from ulcerative colitis patients display<br />
reduced ability to colonize mucus <strong>in</strong> the MSHIME.<br />
Louise Krist<strong>in</strong>e Vigsnaes 1* , Pieter van den Abbeele 2 , Tom van de Wiele 2 and T<strong>in</strong>e Rask Licht 1 .<br />
1 Department <strong>of</strong> Microbiology and Risk Assessment, National Food Institute, Technical University <strong>of</strong><br />
Denmark, Mørkhøj Bygade 19, 2860 Søborg, Denmark.<br />
2 Laboratory <strong>of</strong> Microbial Ecology and Technology (LabMET), Ghent University, Coupure L<strong>in</strong>ks 653,<br />
B‐9000 Gent, Belgium<br />
*Correspond<strong>in</strong>g author: Tel.: +45 35 88 70 24; Fax: +45 35 88 70 28; E‐mail: lokv@food.dtu.dk<br />
Key word: <strong>Ulcerative</strong> colitis, mucus colonization, qPCR, bifidobacteria, lactobacilli, butyrate‐<br />
produc<strong>in</strong>g bacteria, dynamic gut model.<br />
1
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