Role of Intestinal Microbiota in Ulcerative Colitis

Table 2 ‐ 16S rRNA gene and 16S‐23S rRNA intergenic spacer region of species specific primers
used in this study
Target taxon Primer Sequence (5’‐3’) Fragment size (bp) Reference
Faecalibacterium prausnitzii *
Fprau 07 cca tga att gcc ttc aaa act gtt 140 [51]
Fprau 02 gag cct cag cgt cag ttg gt
Akkermansia muciniphila* AM1 cag cac gtg aag gtg ggg ac 327 [9]
AM2 cct tgc ggt tgg ctt cag at
Bifidobacterium bifidum* BiBIF‐1 cca cat gat cgc atg tga ttg 278 [33]
BiBIF‐2 ccg aag gct tgc tcc caa a
Bifidobacterium breve* BiBRE‐1 ccg gat gct cca tca cac 288 [33]
BiBRE‐2 aca aag tgc ctt gct ccc t
Bifidobacterium adolescentis* BiADO‐1 ctc cag ttg gat gca tgt c 279 [33]
BiADO‐2 cga agg ctt gct ccc agt
Bifidobacterium angulatum* BiANG‐1 cag tcc atc gca tgg tgg t 275 [33]
Bifidobacterium
pseudocatenulatum/B.
catenulatum*
BiANG‐2 gaa ggc ttg ctc ccc aac
BiCATg‐1 cgg atg ctc cga ctc ct 289 [33]
BiCATg‐2 cga agg ctt gct ccc gat
Bifidobacterium longum# F_long_IS tgg aag acg tcg ttg gct tt 109
R_long_IS atc gcg cca ggc aaa a
Bacteroides vulgatus* B.vulga‐F aag gga gcg tag atg gat gtt ta 192 [22]
B.vulga‐R cga gcc tca atg tca gtt gc
Bacteroides uniformis* B.uni F2 ata acg agc gca acc ctt atc 190 This study
B.uni R2 tta ggg att agc atc acg tcg
Bacteroides thetaiotaomicron* B_theta F cgt tcc att agg cag ttg gt 110 [47]
B_theta R aca cgg tcc aaa ctc cta cg
Bacteroides distasonis# Bdis‐F tga tcc ctt gtg ctg ct 220 [29]
Bdis‐R atc ccc ctc att cgg a
*Primers targeting the 16S rRNA gene
#Primers targeting the 16S‐23S intergenic spacer region
16
[19]