Role of Intestinal Microbiota in Ulcerative Colitis

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Appendix 3 Methodology part Appendix 3 Additional results for Paper 6: Quantification of Lactobacillus spp. and Bifidobacterium spp. from fermentation samples incubated with DP4, DP5 and FOS. Quantitative Real‐Time PCR was performed using group specific primers targeting Bifidobacterium spp. (Delroisse et al., 2008) and Lactobacillus spp. (Walter et al., 2000;Heilig et al., 2002). DNA extraction and qPCR procedure are performed as described in Paper 6. Log(Relative quantity of 16S rRNA) 6 *** 4 2 0 DP4 # ### DP5 Bifidobacterium spp. FOS Inoculum Log(Relative quantity of 16S rRNA) 5 4 3 2 1 0 DP4 ## DP5 Lactobacillus spp. *** FOS Inoculum Relative quantities of gene targets encoding 16S rRNA from Bifidobacterium spp., Lactobacillus spp. in inoculum and fermentation samples incubated with DP4, DP5 and FOS for 24 hours. Fecal samples were obtained from three healthy subjects, three ulcerative colitis patients in remission and three in relapse. The horizontal lines show the mean of the nine observations. Asterisks indicate a significant difference from inoculum: P
Methodology part Appendix 3 hoc test, Tukey’s multiple comparison test was performed. Tests were considered statistically significant if P‐values lower than 0.05 were obtained. The results revealed that DP4 and DP5 were unable to significantly stimulate the growth of bifidobacteria and lactobacilli after 24 hours of incubation. However, incubation with FOS significantly increased the levels of bifidobacteria and lactobacilli. The level of bifidobacteria was significantly lower after DP4 and DP5 fermentation compared to FOS. This was also applicable for the level of lactobacilli, but only for DP5. 155
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Role of Intestinal Microbiota in Ul
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Role of Intestinal Microbiota in Ul
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Preface Preface This thesis present
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Summary Summary The microbiota of t
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Dansk sammendrag Dansk sammendrag M
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Introduction and objectives Introdu
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List of Manuscripts Not included in
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List of contents List of Centents P
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List of Centents Methodology append
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1. The intestinal environment Theor
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Theoretical part 5 1. The intestina
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2. The colonic environment Theoreti
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Theoretical part 9 2. The colonic e
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Table 1: The presence of glycoside
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Theoretical part Figure 3: The colo
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3. Inflammatory Bowel disease Theor
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Theoretical part 17 3. Inflammatory
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Theoretical part 19 4. Modulation o
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Table 4: Clinical trials on the pre
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Theoretical part 5. Production of p
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Methodology part
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Methodology part 6. Methodology, co
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Introduction Methodology part 42 Pa
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Abstract Background Detailed knowle
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depending the level of disease acti
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in 1 x TAE at 60 °C for 16 h at 36
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Statistics PCA were generated by SA
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The PCA of the Gram‐positive bact
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layer of UC patients and found that
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Acknowledgements The authors thank
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Table 2 ‐ 16S rRNA gene and 16S
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1. Firmicutes phylum 2. Bacteroidet
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Supplementary Figure S1. Dice clust
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Reference List 1. Ahmed S, Macfarla
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32. Matsuki T, Watanabe K, Fujimoto
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Methodology part Paper 2 Fecal lact
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Fecal lactobacilli and bifidobacter
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Introduction The mucus layer lining
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efore enrolment and there was no si
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(Bio‐Rad Labs, Hercules, Californ
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Microbial community analysis using
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difference from the luminal microbi
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that C. coccoides group and C. lept
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Table 1 ‐ 16S rRNA gene of phylum
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Table 2 ‐ Preference of bacterial
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Figure 1. A) Schematic overview of
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A. B. Figure 3. Principal component
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15. Fooks LJ, Gibson GR. (2002) In
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47. Ouwehand AC, Suomalainen T, Tol
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Methodology part Paper 3 Paper 3 In
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APPLIED AND ENVIRONMENTAL MICROBIOL
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8338 VIGSNÆS ET AL. APPL. ENVIRON.
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Page 195 and 196: References Pullan,R.D., Thomas,G.A.
Page 197 and 198: References Tannock,G.W. (2010). Ana
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Role of Intestinal Microbiota in Ulcerative Colitis

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