Role of Intestinal Microbiota in Ulcerative Colitis

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Methodology part 152 Appendix 1 horizontal lines show the mean of the three observations and the error bars represent standard deviation for the three healthy subjects. The data was log‐transformed before statistical analysis to obtain normal distribution. Univariate ANOVA was used to analyze difference in days. Tests were considered statistically significant if P‐ values lower than 0.05 were obtained.
Appendix 2 Methodology part Quantification of eight bacterial taxa to validate the combined DNA extraction method Appendix 2 Quantitative Real‐Time PCR was performed using primers targeting Bifidobacterium spp. (Delroisse et al., 2008), Lactobacillus spp. (Walter et al., 2000;Heilig et al., 2002), Coccoides leptum subgroup (Walter et al., 2000;Matsuki et al., 2004;Shen et al., 2006), Firmicutes (Guo et al., 2008), Desulfovibrio spp. (Fite et al., 2004), Bacteroidetes (Guo et al., 2008), Bacteroides spp. (Ahmed et al., 2007), Alistipes spp. (Paper 1) and. DNA extraction and qPCR procedure are performed as described in Paper 2‐6. Log(Relative quantity of 16S rRNA) 5 4 3 2 1 0 Bifidobacterium spp. Lactobacillus spp. Bifidobacterium spp. Lactobacillus spp. C. leptum subgroup Gram-positive bacteria C. leptum subgroup Firmicutes Firmicutes Log(Relative quantity of 16S rRNA) 5 4 3 2 1 0 Desulfovibrio spp. Desulfovibrio spp. Bacteroidetes Bacteroidetes Gram-negative bacteria Bacteroides spp. Bacteroides spp. Alistipes spp. Alistipes spp. Relative quantities of gene targets encoding 16S rRNA from four Gram‐positive bacteria and four Gram‐negative bacteria in either 0 hours fermentation samples (white) or fecal samples (hatched). Samples were obtained from three healthy subjects. Error bars represent averages ± standard deviation of the three different subjects. The data was log‐transformed before statistical analysis to obtain normal distribution. Two‐tailed pair wise t‐test was used to analyze difference in samples. Tests were considered statistically significant if P‐values lower than 0.05 were obtained. 153
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Role of Intestinal Microbiota in Ul
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Role of Intestinal Microbiota in Ul
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Preface Preface This thesis present
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Summary Summary The microbiota of t
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Dansk sammendrag Dansk sammendrag M
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Introduction and objectives Introdu
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List of Manuscripts Not included in
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List of contents List of Centents P
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List of Centents Methodology append
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1. The intestinal environment Theor
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Theoretical part 5 1. The intestina
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2. The colonic environment Theoreti
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Theoretical part 9 2. The colonic e
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Table 1: The presence of glycoside
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Theoretical part Figure 3: The colo
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3. Inflammatory Bowel disease Theor
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Theoretical part 17 3. Inflammatory
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Theoretical part 19 4. Modulation o
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Table 4: Clinical trials on the pre
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Theoretical part 5. Production of p
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Methodology part
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Methodology part 6. Methodology, co
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Introduction Methodology part 42 Pa
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Abstract Background Detailed knowle
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depending the level of disease acti
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in 1 x TAE at 60 °C for 16 h at 36
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Statistics PCA were generated by SA
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The PCA of the Gram‐positive bact
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layer of UC patients and found that
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Acknowledgements The authors thank
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Table 2 ‐ 16S rRNA gene and 16S
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1. Firmicutes phylum 2. Bacteroidet
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Supplementary Figure S1. Dice clust
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Reference List 1. Ahmed S, Macfarla
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32. Matsuki T, Watanabe K, Fujimoto
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Methodology part Paper 2 Fecal lact
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Fecal lactobacilli and bifidobacter
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Introduction The mucus layer lining
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efore enrolment and there was no si
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(Bio‐Rad Labs, Hercules, Californ
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Microbial community analysis using
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difference from the luminal microbi
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that C. coccoides group and C. lept
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Table 1 ‐ 16S rRNA gene of phylum
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Table 2 ‐ Preference of bacterial
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Figure 1. A) Schematic overview of
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A. B. Figure 3. Principal component
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15. Fooks LJ, Gibson GR. (2002) In
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47. Ouwehand AC, Suomalainen T, Tol
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Methodology part Paper 3 Paper 3 In
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APPLIED AND ENVIRONMENTAL MICROBIOL
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Page 195 and 196: References Pullan,R.D., Thomas,G.A.
Page 197 and 198: References Tannock,G.W. (2010). Ana
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Role of Intestinal Microbiota in Ulcerative Colitis

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