Role of Intestinal Microbiota in Ulcerative Colitis

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Methodology part 150
Appendix 1 Methodology part Quantification of bacterial taxa at five different occasions Appendix 1 Quantitative Real‐Time PCR was performed using primers targeting Firmicutes (Guo et al., 2008), Bacteroidetes (Guo et al., 2008), Bifidobacterium spp. (Delroisse et al., 2008) and Lactobacillus spp. (Walter et al., 2000;Heilig et al., 2002). DNA extraction and qPCR procedure are performed as described in Paper 2‐6. Log(Relative quantity of 16S rRNA) Log(Relative quantity of 16S rRNA) 5.5 5.0 4.5 4.0 3.5 3.0 5.5 5.0 4.5 4.0 3.5 day1 day1 day2 day2 day3 day3 Bifidobacterium spp. day4 day4 day5 Firmicutes day5 Log(Relative quantity of 16S rRNA) 5 4 3 2 1 0 day1 day1 day2 day2 day3 day3 Lactobacillus spp. day4 day4 day5 Bacteroidetes Relative quantities of gene targets encoding 16S rRNA from Bifidobacterium spp., Lactobacillus spp., Firmicutes and Bacteroidetes in fermentation samples incubated with FOS for 24 hours at five different occasions (day1‐day5). Fecal samples were obtained from three healthy subjects. The 151 Log(Relative quantity of 16S rRNA) 5.0 4.5 4.0 3.5 3.0 day5
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Role of Intestinal Microbiota in Ul
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Role of Intestinal Microbiota in Ul
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Preface Preface This thesis present
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Summary Summary The microbiota of t
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Dansk sammendrag Dansk sammendrag M
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Introduction and objectives Introdu
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List of Manuscripts Not included in
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List of contents List of Centents P
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List of Centents Methodology append
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1. The intestinal environment Theor
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Theoretical part 5 1. The intestina
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2. The colonic environment Theoreti
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Theoretical part 9 2. The colonic e
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Table 1: The presence of glycoside
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Theoretical part Figure 3: The colo
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3. Inflammatory Bowel disease Theor
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Theoretical part 17 3. Inflammatory
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Theoretical part 19 4. Modulation o
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Table 4: Clinical trials on the pre
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Theoretical part 5. Production of p
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Methodology part
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Methodology part 6. Methodology, co
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Introduction Methodology part 42 Pa
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Abstract Background Detailed knowle
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depending the level of disease acti
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in 1 x TAE at 60 °C for 16 h at 36
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Statistics PCA were generated by SA
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The PCA of the Gram‐positive bact
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layer of UC patients and found that
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Acknowledgements The authors thank
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Table 2 ‐ 16S rRNA gene and 16S
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1. Firmicutes phylum 2. Bacteroidet
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Supplementary Figure S1. Dice clust
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Reference List 1. Ahmed S, Macfarla
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32. Matsuki T, Watanabe K, Fujimoto
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Methodology part Paper 2 Fecal lact
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Fecal lactobacilli and bifidobacter
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Introduction The mucus layer lining
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efore enrolment and there was no si
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(Bio‐Rad Labs, Hercules, Californ
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Microbial community analysis using
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difference from the luminal microbi
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that C. coccoides group and C. lept
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Table 1 ‐ 16S rRNA gene of phylum
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Table 2 ‐ Preference of bacterial
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Figure 1. A) Schematic overview of
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A. B. Figure 3. Principal component
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15. Fooks LJ, Gibson GR. (2002) In
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47. Ouwehand AC, Suomalainen T, Tol
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Methodology part Paper 3 Paper 3 In
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Page 177 and 178: 7. Discussion and perspectives Disc
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Page 185 and 186: References References Abreu,M.T., V
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Page 193 and 194: References Matsuki,T., Watanabe,K.,
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Page 197 and 198: References Tannock,G.W. (2010). Ana
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Role of Intestinal Microbiota in Ulcerative Colitis

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