Role of Intestinal Microbiota in Ulcerative Colitis
Role of Intestinal Microbiota in Ulcerative Colitis Role of Intestinal Microbiota in Ulcerative Colitis
Table 1 continues Eubacterium rectale Eu‐rec_1F aag gga agc aac gct gtg aa 200 [4] Eu‐rec_2R Cgg tta ggt cac tgg ctt c Akkermansia muciniphila AM1 cag cac gtg aag gtg ggg ac 327 [8] AM2 cct tgc ggt tgg ctt cag at Bifidobacterium bifidum BiBIF‐1 cca cat gat cgc atg tga ttg 278 [41] BiBIF‐2 ccg aag gct tgc tcc caa a Bifidobacterium adolescentis BiADO‐1 ctc cag ttg gat gca tgt c 279 [41] Bifidobacterium pseudocatenulatum/ Bifidobacterium catenulatum BiADO‐2 cga agg ctt gct ccc agt BiCATg‐1 cgg atg ctc cga ctc ct 289 [41] BiCATg‐2 cga agg ctt gct ccc gat *The HDA and TBA primer were used as total bacteria DNA targets in order to normalize, hence correcting differences in total DNA concentration between individual samples. 16
Table 2 ‐ Preference of bacterial phylum/group/species to colonize mucosal over the luminal compartment, expressed as ratio of the relative quantities in the mucosal and luminal compartment (%) Ratios (%) b Log (average) Healthy subjects UC remission UC relapse 100.36 (±1.23) 100.46 (±1.64) 99.85 (±0.89) 96.33 (±8.43) 83.81 (±5.12)* 82.39 (±3.35)* 102.62 (±13.35) 83.75 (±4.22)* 93.91 (±4.97) 88.47 (±10.94) 74.10 (±11.74) 66.81 (±10.73)* 108.01 (±7.70) 137.83 (±12.28)# 146.06 (±41.24) 128.02 (±11.17)# 149.25 (±15.99)* 104.60 (±9.57) 94.70 (±8.85) 81.32 (±2.77)** 68.59 (±7.64)* 99.98 (±2.84) 106.52 (±9.69) 94.36 (±7.57) 98.44 (±2.87) 112.93 (±9.33) 86.17 (±4.66)# 65.05 (17.58) 68.12 (±11.76)* 71.91 (±8.47)* a Bacterial taxa Lumen Mucus Firmicutes 7.70 (±0.07) 7.71 (±0.03) • Clostridium leptum subgroup 6.27 (±0.30) 5.41 (±0.21) o Faecalibacterium prausnitzii 5.08 (±0.34) 4.61 (±0.26) • Clostridium coccoides group 5.53 (±0.33) 4.29 (±0.29) o Roseburia spp. 3.39 (±0.24) 4.31 (±0.47) o Eubacterium rectal 2.55 (±0.21) 3.21 (±0.28) • Lactobacillus spp. 6.39 (±0.40) 5.20 (±0.40) Bacteroidetes 5.65 (±0.23) 5.58 (±0.15) • Bacteroides spp. 5.46 (±0.24) 5.33 (±0.19) • Alistipes spp. 4.62 (±0.21) 3.22 (±0.37) Actinobacteria 17 86.17 (±2.24)** 91.24 (±1.53)** 94.60 (±3.12) 6.67 (±0.18) 7.35 (±0.11) 73.86 (±7.58)# 100.69 (±5.15) 90.82 (±5.05) 3.99 (±0.55) 4.48 (±0.55) • Bifidobacterium spp. o B. bifidum 58.66 (±22.92)* 78.43 (±1.88)** 85.99 (±6.14) 4.65 (±0.27) 5.43 (±0.47) o B. adolescentis 94.60 (±15.00) 79.79 (±3.55)* 101.27 (±4.68) 4.54 (±0.45) 5.31 (±0.55) o B. pseudocatenulatum Proteobacteria 92.74 (±3.89) 101.22 (±4.53) 101.19 (±1.75) 3.49 (±0.23) 3.56 (±0.24) • Desulfovibrio spp. Verrucomicrobia 69.81 (±10.46) 73.49 (±10.30) 71.03 (±15.02) 2.48 (±0.38) 3.68 (±0.36) • Akkermansia muciniphila a Logarithmic average of relative quantities for healthy subjects and UC patients from luminal or mucosal bacteria levels. b Ratios (%) calculated as 100*mucosal samples/luminal samples, hence below 100%, low adherence capacity to mucus and above 100%, high adherence capacity to mucus. B. pseudocatenulatum is both B. catenulatum and B. pseudocatenulatum Asterisks (*) indicate significant differences between mucosal and luminal samples (*P
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Table 1 cont<strong>in</strong>ues<br />
Eubacterium rectale Eu‐rec_1F aag gga agc aac gct gtg aa 200 [4]<br />
Eu‐rec_2R Cgg tta ggt cac tgg ctt c<br />
Akkermansia muc<strong>in</strong>iphila AM1 cag cac gtg aag gtg ggg ac 327 [8]<br />
AM2 cct tgc ggt tgg ctt cag at<br />
Bifidobacterium bifidum BiBIF‐1 cca cat gat cgc atg tga ttg 278 [41]<br />
BiBIF‐2 ccg aag gct tgc tcc caa a<br />
Bifidobacterium adolescentis BiADO‐1 ctc cag ttg gat gca tgt c 279 [41]<br />
Bifidobacterium<br />
pseudocatenulatum/<br />
Bifidobacterium catenulatum<br />
BiADO‐2 cga agg ctt gct ccc agt<br />
BiCATg‐1 cgg atg ctc cga ctc ct 289 [41]<br />
BiCATg‐2 cga agg ctt gct ccc gat<br />
*The HDA and TBA primer were used as total bacteria DNA targets <strong>in</strong> order to normalize, hence correct<strong>in</strong>g differences<br />
<strong>in</strong> total DNA concentration between <strong>in</strong>dividual samples.<br />
16
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