Role of Intestinal Microbiota in Ulcerative Colitis
Role of Intestinal Microbiota in Ulcerative Colitis Role of Intestinal Microbiota in Ulcerative Colitis
Acknowledgements The authors thank Kate Vibefeldt for excellent technical assistance. Additionally, we thank Jørn Brynskov and Casper Steenholdt, Herlev Hospital, for assistance with collection of fecal samples. Pieter Van den Abbeele is a Postdoctoral fellow from FWO‐Vlaanderen (Research Foundation of Flanders, Belgium). Finally, this work was financed by the Danish Council for Strategic Research (Grant no. 2101‐06‐0067), the Technical University of Denmark and a GOA (BOF07/GOA/002) project from Ghent University. 14
Table 1 ‐ 16S rRNA gene of phylum, group and species specific primers Target taxon Primer Sequence (5’‐3’) Fragment size (bp) Reference Bifidobacterium spp. F‐bifido cgc gtc ygg tgt gaa ag 244 [10] R‐bifido ccc cac atc cag cat cca Lactobacillus spp. Lacto‐F agc agt agg gaa tct tcc a 341 [20,65] Lacto‐R cac cgc tac aca tgg ag Clostridium leptum subgroup sg‐Clept‐F gca caa gca gtg gag t 239 [40,56] sg‐Clept‐R ctt cct ccg ttt tgt caa Clostridium coccoides group g‐Ccoc‐F aaa tga cgg tac ctg act aa 440 [40] g‐Ccoc‐R ctt tga gtt tca ttc ttg cga a Roseburia spp. RosF tac tgc att gga aac tgt cg 230 [32] RosR cgg cac cga aga gca at Firmicutes phylum Firm934F gga gya tgt ggt tta att cga agc a 126 [19] Firm1060R agc tga cga caa cca tgc ac Bacteroidetes phylum Bact934F gga rca tgt ggt tta att cga tga t 126 [19] Bact1060R agc tga cga caa cca tgc ag Bacteroides spp. BacAll_2F gtc agt tgt gaa agt ttg c 127 [1] BacAll_2R caa tcg gag ttc ttc gtg Alistipes spp. Alis F1‐124 tta gag atg ggc atg cgt tgt 320 [64] Alis R1‐423 tga atc ctc cgt att acc gcg Desulfovibrio spp. DSV691‐F ccg tag ata tct gga gga aca tca g 136 [14] DSV826‐R aca tct agc atc cat cgt tta cag c V2‐V3 16S rRNA region* HDA1 act cct acg gga ggc agc agt 200 [65] HDA2 gta tta ccg cgg ctg ctg gca c 16S rRNA region* TBA‐F cgg caa cga gcg caa ccc 130 [11] TBA‐R cca ttg tag cac gtg tgt agc c Faecalibacterium prausnitzii Fprau 07 cca tga att gcc ttc aaa act gtt 140 [57] Fprau 02 gag cct cag cgt cag ttg gt 15
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Acknowledgements<br />
The authors thank Kate Vibefeldt for excellent technical assistance. Additionally, we thank Jørn<br />
Brynskov and Casper Steenholdt, Herlev Hospital, for assistance with collection <strong>of</strong> fecal samples.<br />
Pieter Van den Abbeele is a Postdoctoral fellow from FWO‐Vlaanderen (Research Foundation <strong>of</strong><br />
Flanders, Belgium). F<strong>in</strong>ally, this work was f<strong>in</strong>anced by the Danish Council for Strategic<br />
Research (Grant no. 2101‐06‐0067), the Technical University <strong>of</strong> Denmark and a GOA<br />
(BOF07/GOA/002) project from Ghent University.<br />
14
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