Proceedings of the 10th International Colloquium on Paratuberculosis
Proceedings of the 10th International Colloquium on Paratuberculosis
Proceedings of the 10th International Colloquium on Paratuberculosis
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A statistically significant lower frequency in MAP infecti<strong>on</strong> was observed in animals<br />
older than 5 years compared to 3-5 years-old animals (pELISA=0.0002; pCULTURE=0.0087; pRTi-<br />
PCR=0.0078; pHP< 0.0001).<br />
A. B.<br />
NUMBER OF SAMPLES (n)<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
25.0<br />
10.4<br />
16.5<br />
10.8<br />
5.9<br />
3.0<br />
4.0<br />
7.4<br />
11.1<br />
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11<br />
AGE (YEARS)<br />
C. D.<br />
NUMBER OF SAMPLES (n)<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
25.0<br />
18.7<br />
26.8<br />
25.0<br />
18.8<br />
13.4<br />
10.9<br />
12.0<br />
22.2<br />
11.1<br />
26.3<br />
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11<br />
AGE (YEARS)<br />
45<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
45<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
PTB PREVALENCE (%) .<br />
PTB PREVALENCE (%) .<br />
NUMBER OF SAMPLES (n)<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
140<br />
120<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
25.0<br />
18.7<br />
27.1<br />
21.8<br />
18.8<br />
8.9 9.1<br />
12.0<br />
23.1<br />
16.7<br />
23.5<br />
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11<br />
33.3<br />
18.7<br />
42.3<br />
25.0<br />
AGE (YEARS)<br />
6.1<br />
4.0<br />
14.3<br />
1,5-2 2-3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 >11<br />
AGE (YEARS)<br />
Fig. 1. Age structure <str<strong>on</strong>g>of</str<strong>on</strong>g> MAP infecti<strong>on</strong> in relati<strong>on</strong> to diagnostic tests: ELISA (A), culture (B),<br />
RTi-PCR (C) and histopathological examinati<strong>on</strong> (D). Line: MAP prevalence and bars: number<br />
<str<strong>on</strong>g>of</str<strong>on</strong>g> samples tested.<br />
Table 1. Sensitivity, complementary sensitivity and agreement (�) values <str<strong>on</strong>g>of</str<strong>on</strong>g> diagnostic test<br />
combinati<strong>on</strong>s. Interpretati<strong>on</strong> <str<strong>on</strong>g>of</str<strong>on</strong>g> agreement: poor (�=0.00-0.20), fair (�=0.21-0.40), moderate<br />
(�=0.41-0.60), good (�=0.61-0.80) and excellent (�=0.81-1.00).<br />
Sensitivity (%) Complementary sensitivity (%)<br />
EVALUATED<br />
REFERENCE TEST<br />
REFERENCE TEST<br />
TEST ELISA CULTURE RTi-PCR HP ELISA CULTURE RTi-PCR HP<br />
ELISA<br />
CULTURE<br />
RTi-PCR<br />
NUMBER OF SAMPLES (n)<br />
- 37.0 37.1 72.4 - 6.5 4.3 24.1<br />
(�=0.454) (�=0.461) (�=0.696)<br />
85.1 - 63.4 85.7 144.7 - 33.0 92.9<br />
(�=0.562) (�=0.536)<br />
89.6 65.7 - 86.2 152.0 38.0 - 96.5<br />
(�=0.525)<br />
HP 75.0 48.0 47.2 - 28.6 8.0 7.5 -<br />
Broadly, diagnostic test combinati<strong>on</strong>s resulted in moderate agreement (�=0.41-0.60)<br />
excepting for <str<strong>on</strong>g>the</str<strong>on</strong>g> combinati<strong>on</strong> ELISA and histopathological examinati<strong>on</strong> that showed a good<br />
187<br />
45<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
45<br />
40<br />
35<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
0<br />
PTB PREVALENCE (%) .<br />
PTB PREVALENCE (%) .