Insect Control: Biological and Synthetic Agents - Index of

132 4: Insect Growth- and Development-Disrupting Insecticides
Table 5 Relative binding affinities of bisacylhydrazine compounds to cellular extracts or in vitro expressed EcR and USP proteins
from insects of different orders
20E Pon A RH-5849 RH-5992 RH-2485 RH-0345 Reference
Drosophila melanogaster 145 nM 0.9 nM 336 nM Dhadialla,
(unpublished
data), Cherbas
et al. (1988)
Aedes aegypti 28 nM 2.8 nM 30 nM Dhadialla,
unpublished data;
Kapitskaya et al.
(1996)
Chironomus tentans 0.35 0.28 and
6.5 2.4 nM
Grebe et al. (2000),
Smagghe et al.
(2001)
Spodoptera littoralis 158 nM 86.7 nM 24.3 nM Smagghe et al.
(2000a)
Plodia interpunctella 210 nM 3 nM 3 nM Dhadialla,
unpublished data
Galleria mellonella 106 nM 911 nM 22 nM Smagghe et al.
(1996a)
Spodoptera frugiperda (Sf9 166 nM 8.9 nM 363 nM 1.5 nM 3.5 nM Nakagawa et al.
cells)
(2000)
Anthonomus grandis 247 nM 6.1 nM 12 000 nM Dhadialla and
Tzertzinis (1997)
Leptinotarsa decemlineata 425 nM 740 nM 1316 nM Smagghe et al.
(1996a)
Tenebrio molitor 6nM >10 mM >10 mM >10 mM >10 mM Dhadialla,
unpublished data
Locusta migratoria 1000 nM 1.18 nM >10 mM >10 mM >10 mM >10 mM Hayward et al. (2003)
Bamesia argentfolli 8nM >10 mM >10 mM >10 mM >10 mM Dhadialla,
unpublished data
Figure 7 Schematic diagram showing a yeast cell transformed with plasmids carrying cDNAs encoding ecdysone receptor, RXRg,
co-activator glucocorticoid receptor interaction protein (GRIP), and b-galactosidase (b-Gal) reporter gene fused to 6 heat shock
protein 27 (hsp27) ecdysone response elements in a reconstituted and ecdysteroid (muristerone A) inducible high throughput
screening system for discovery of new nonsteroidal ecdysone agonists.