International Journal of Noni Research - Noni Family
International Journal of Noni Research - Noni Family
International Journal of Noni Research - Noni Family
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39 Intl. J. <strong>Noni</strong> Res. 2007, 2(1-2)<br />
stage plants were brought to Tissue Culture Laboratory and used for explant<br />
collection. Apical regions and nodal explants were used. Young leaves were<br />
used for callus initiation.<br />
Sterilization details<br />
a. Nodal explant<br />
The nodal explants were collected from 6-8 months old seedlings. Top 3 nodes<br />
were taken for tissue culture purpose by leaving the basal single node in the<br />
mother plant. Due to the removal <strong>of</strong> upper axillary buds, the axillary region<br />
sprouts very efficiently by producing two axillary sprouts in vivo within 8-10 days.<br />
b. Procedure<br />
Top 3 nodes were taken and then washed with sterile water and kept in 1:<br />
2 (Sodium hypochlorite : Sterile water) with constant stirring for 30 minutes.<br />
The ex-plants were washed 3-4 times with sterile water, then treated with<br />
0.1% mercuric chloride for 5 minutes and rinsed with sterile water before<br />
inoculation.<br />
c. Leaf ex–plant<br />
The above sterilization treatment was given for leaf ex plant also to initiate<br />
callus.<br />
Media details<br />
J. Subramani et al. Micropropagation <strong>of</strong> Morinda citrifolia L.<br />
Fig.1<br />
Fig.2<br />
Following basal media with various combinations <strong>of</strong> Cytokinin and Auxin<br />
were tried in our in-vitro studies. The results are shown in Table 1, 2 & 3.<br />
Table 1. Various combinations <strong>of</strong> media for in-vitro Multiplication<br />
Media BAP (mg/I) KN (mg/I) IBA (mg/I)<br />
2.0 - -<br />
4.0 - -<br />
MS 1.0 1.0 -<br />
2.0 0.75 2.0<br />
1.0 0.25 -