Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_

Poster
Topic: Sports Medicine - Systemic
Abstract number: 25481
EFFECTS OF COMMONLY USED DRUGS IN SPORTS MEDICINE ON GROWTH
AND CELLULAR APPEARANCE OF MESENCHYMAL STEM CELLS (MSC)
Maritha FREDRIKSSON, Lars-Arne HALDOSEN, Li TSAI
Karolinska Institutet, Stockholm (SWEDEN)
Joint injuries cause pain, inflammation and dysfunction. Pharmacologic treatment is
often initiated. There is a need to clarify the local tissue effects of these drugs and
their impact on MSC as the precursor to tendons, bone and cartilage. The aim of this
study was to investigate the effects of commonly used drugs in sports medicine on
growth and appearance of MSC in culture.The set-up was an in vitro-study of murine
MSC’s. Pure active substance was added to the cultures (commonly used
corticosteroids, local anaesthetics and NSAID). Growth over time was measured by
spectrophotometry at set points. Cellular appearance was observed by microscopy
and photo-documented.In contrast to local anaesthetics, commonly used NSAID’s
caused inhibition of growth and also affected the cellular appearance of MSC’s in
culture. The result was dose dependent. The most prominent inhibition of growth was
observed by glucocorticoid treatment. This effect was also dose dependent.
Commonly used drugs in sports medicine have pronounced biological effects on
MSC function which are the precursors for mesenchymal tissue such as bone,
cartilage and tendon. The most prominent effects were caused by glucocorticoids
and NSAID. These effects might have long term influence on regeneration after
tissue injury and must be taken into consideration before initiating pharmacological
treatment. There is a need for new targets for treatment optimizing the balance
between pain, inflammation and regeneration.
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