Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_

Poster
Topic: Sports Medicine - Knee
Abstract number: 25741
PROPHYLACTIC EFFECT OF CARBOXYMETHYLCELLULOSE HYALURONANE
(SEPRAFILM®) MEMBRANE ON THE KNEE ARTHROFIBROSIS
(EXPERIMENTAL
ANIMAL STUDY)
TUGAY YAGCI 1 , SEMIH AYDOGDU 1 , MURAT SEZAK 2 , AYLIN ORAL 3
1 Ege University Hospital, Dept of Orthopaedic Surgery, IZMIR (TURKEY), 2 Ege
University Hospital, Dept of Pathology, IZMIR (TURKEY), 3 Ege University Hospital,
Dept of Nuclear Medicine, IZMIR (TURKEY)
We aimed to investigate the effect of Seprafilm®, a synthetic biomembran, on the
intra-articular adhesion formation in an experimental arthrofibrosis model. Twenty
male white rabbits were randomly allocated into two groups. A standard surgical
procedure to develop arthrofibrosis (medial parapatellar arthrotomy, lateral eversion
of patella, partial synovectomy, debridement of anterior supracondylar area and
patella joint surface by scalpel) was performed on all rabbits’ right knees. Group 1
rabbits served as controls, and in Group 2 rabbits Seprafilm® placed into the
described area. In both groups, after surgery, knee joint was immobilized by no.5
wire suture passing from the ankle and groin and keeping the joint in 140° of flexion.
At 6th week, all animals were sacrificed and adhesion formation was evaluated both
macroscopically and histo-pathologically. All data were semi-quantified and analyzed
statistically by Fisher’s exact test. While all rabbits in control group displayed
different rates of adhesion macroscopically (62.5% severe, 25% moderate, 12.5%
mild), none in the study group had it. The average macroscopic adhesion score was
2.5 ± 0.75 in control group, and 0 in Seprafilm® group. Histopathologic evaluation
also revealed microscopic adhesion in all rabbits in control group, but none in
Seprafilm® group. Fibroblast proliferation in Seprafilm® group (100% mild) was
significantly lower than in control group (62.5% severe, 37.5% moderate) (p