Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_

Poster
Topic: General Orthopaedics
Abstract number: 25939
A NOVEL SINGLE PULSED ELECTROMAGNETIC FIELD STIMULATES THE
OSTEOGENESIS AND ANGIOGENESIS
Yin-Chih FU, Yuh-Min CHENG, Mei-Ling HO
Kaohsiung Medical University Hospital, Kaohsiung (TAIWAN)
Pulsed electromagnetic field (PEMF) has been successfully applied for accelerating
fracture repair as early as 1979. Since the treatment required 8-12 hrs of application
daily, the inconvenience leads us to develop a newly designed single pulsed
electromagnetic field (SPEMF). Materials and Methods: The single pulsed
electromagnetic field (SPEMF) is in composition of a single pulsed repeated in
adjustable times and magnetic fields. Pulsed period is 5 ms in sine wave per
stimulation. Thymidine incorporation was used to examine cell proliferation. ALP and
mineralization assay were used to evaluate osteogenic differentiation of hBMSCs.
Balb/C mice were used to validate the effects of SPEMF. We evaluated the x-ray,
H&E stain to count bone matrix ratio and vWF stain to count the angiogenesis ability.
Results: The ALP activity of hBMSCs was significantly increased in SPEMF-treated
groups after 2-5 days of treatment. The mineralization was also increased after 15-25
days of SPEMF stimulation. In animal study also had the same results. Discussion: In
this study, we demonstrated there is a novel electromagnetic field which showed
advantages not only increased proliferation of hBMSCs, osteogenic differentiation of
hBMSCs, but also accomplished the desire goal in less than 3 min of treatment per
day. In conclusion, our result showed that SPEMF would increase proliferation of
hBMSCs in a proliferative environment, increased differentiation of hBMSCs in an
osteogenic environment, and accelerated mineralization in a mimic physiologic
environment. SPEMF could be a useful device in enhancing fracture healing.
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