Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_
Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_ Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_
Poster Topic: Cartilage Repair Abstract number: 26765 CHONDROCYTE GENE EXPRESSION IS AFFECTED BY VSOP-LABELING IN LONG-TERM IN VITRO MRI TRACKING Casper FOLDAGER, Michael PEDERSEN, Steffen RINGGAARD, Cody BÜNGER, Martin LIND Aarhus University Hospital, Aarhus (DENMARK) INTRODUCTION: Autologous chondrocyte transplantation is an essential part of many treatment procedures for cartilage repair. The aim was to investigate the effect and dose-response of very-small iron oxide particle (VSOP) labeling of human chondrocytes for long-term in vitro MRI tracking. METHODS: Chondrocytes were isolated from cartilage biopsies from four patients. The cells for the dose-response study were labeled with 25, 50 or 100 µg/mL VSOP. Quantitative gene expression and cellular proliferation were compared to unlabeled controls at day 1, 3, and 7. The cells suited for MRI tracking were labeled with 50 µg/mL VSOP and embedded in alginate beads, followed by MRI (using T2-weighted sequences) at day 0, 1, 3, 7, 14, 21, 28, and histology was performed at each time-point. RESULTS Histology revealed that VSOP particles were intracellularly confined at all time-points, whereas no extracellular VSOP’s were observed. A mean reduction in T2-value of 25.1 ms (±SD 3.5 ms) was found on T2-maps. The chondrocyte-specific genes aggrecan, collagen type 2, and sox9 were all affected by labeling, the two latter in a dosedependent manner. VSOP’s had no effect on proliferation. CONCLUSION: VSOPlabeling of chondrocytes affected gene expression but not proliferation. The labeled chondrocytes could be recognized by MRI for 4 weeks without significant changes in the T2 relaxation time. 200
Poster Topic: Cartilage Repair Abstract number: 26775 A SINGLE STAGE PROCEDURE FOR CELL-BASED CARTILAGE REPAIR: 24 MONTH RESULTS FROM A PILOT STUDY F. ALMQVIST 1 , T. SPALDING 2 , M. BRITTBERG 3 , S. NEHRER 4 , A. IMHOFF 5 , J. FARR 6 , B.J. COLE 7 1 2 Ghent University Hospital, GENT (BELGIUM), Hospital of St Cross, Warwickshire 3 (UNITED KINGDOM), Kungsbacka Hospital, Kungsbacka (SWEDEN), 4 Krankenhaus Krems, Krems (AUSTRIA), 201 5 Abteilung und Poliklinik für Sportorthopädie, München (GERMANY), 6 OrthoIndy, Indianapolis (INDIA), 7 Rush University Medical Center (ISRAEL) Cartilage autologous implantation system (CAIS) is a surgical method in which hyaline cartilage fragments from a non-weight bearing area in the knee joint are collected and then precipitated onto an absorbable filter that is subsequently placed in the focal chondral defect. The clinical outcome of CAIS was compared with microfracture (MFX) in a pilot study. In an IRB approved protocol patients (n=29) were screened with the intention to treat, randomized (2:1, CAIS:MFX) and followed over a 24 month period. To be included in the study the patient may have up to 2 contained focal, unipolar lesions (ICRS grade 3d and ICRS Grade IVa OCD lesions of femoral condyles and trochlea with a size between 1 and 10 cm2. We report 24 month patient-reported outcome (PRO) data using the KOOS-scale. We noted that at 12 months after the intervention CAIS differentiated itself from MFX in that the changes in Sport & Recreation subscale were different (p
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Poster<br />
Topic: Cartilage Repair<br />
Abstract number: 26775<br />
A SINGLE STAGE PROCEDURE FOR CELL-BASED CARTILAGE REPAIR: 24<br />
MONTH RESULTS FROM A PILOT STUDY<br />
F. ALMQVIST 1 , T. SPALDING 2 , M. BRITTBERG 3 , S. NEHRER 4 , A. IMHOFF 5 , J.<br />
FARR 6 , B.J. COLE 7<br />
1 2<br />
Ghent University Hospital, GENT (BELGIUM), Hospital of St Cross, Warwickshire<br />
3<br />
(UNITED KINGDOM), Kungsbacka Hospital, Kungsbacka (SWEDEN),<br />
4 Krankenhaus Krems, Krems (AUSTRIA),<br />
201<br />
5 Abteilung und Poliklinik für<br />
Sportorthopädie, München (GERMANY), 6 OrthoIndy, Indianapolis (INDIA), 7 Rush<br />
University Medical Center (ISRAEL)<br />
Cartilage autologous implantation system (CAIS) is a surgical method in which<br />
hyaline cartilage fragments from a non-weight bearing area in the knee joint are<br />
collected and then precipitated onto an absorbable filter that is subsequently placed<br />
in the focal chondral defect. The clinical outcome of CAIS was compared with<br />
microfracture (MFX) in a pilot study. In an IRB approved protocol patients (n=29)<br />
were screened with the intention to treat, randomized (2:1, CAIS:MFX) and followed<br />
over a 24 month period. To be included in the study the patient may have up to 2<br />
contained focal, unipolar lesions (ICRS grade 3d and ICRS Grade IVa OCD lesions<br />
of femoral condyles and trochlea with a size between 1 and 10 cm2. We report 24<br />
month patient-reported outcome (PRO) data using the KOOS-scale. We noted that<br />
at 12 months after the intervention CAIS differentiated itself from MFX in that the<br />
changes in Sport & Recreation subscale were different (p
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