Abstracts Posters SICOT-SOF meeting Gothenburg 2010 _2_

Poster
Topic: Biomaterials
Abstract number: 26671
IN VIVO TESTING OF STRUCTURAL GRADED POLYCAPROLACTONE
SCAFFOLDS IN A PORCINE CALVARIA MODEL
Jonas JENSEN 1 , Michael BENDTSEN 1 , Jens Vinge NYGAARD 2 , Dang Quang Svend
LE 2 , Lea BJERRE 1 , Cody Eric BÜNGER 1
1 Orthopaedic Research Lab, Aarhus University Hospital, Århus (DENMARK),
2 Interdisciplinary Nano Science Center (iNANO), Aarhus University, Århus
(DENMARK)
Background: The scaffolds were comprised of the polymer, polycaprolactone (PCL).
The pure bioplotted scaffolds were cylinder-shaped (10 mm diameter x 10 mm
height). Scaffolds were infused with a homogenous mixture of PCL, water and 1,4dioxane
and underwent a thermal induced phase separation (TIPS) followed by
lyophilization to manufacture a structurally-graded-scaffold (SGS) with micro-and
nanopore PCL formation within the gaps of the bioplotted scaffold. Materials and
Methods: A total of 16 Danish landrace pigs were used with termination of eight pigs
after 8 and 12 weeks. A total of six non-penetrating critical size defects were drilled in
the calvaria. The following scaffolds were placed in the bottom of each drill hole by
random: 1.) Autograft (1,12 g harvested from the drill holes) 2.) Empty hole, 3.)
Bioplotted scaffold, 4.) SGS scaffold, 5.) SGS scaffold + autologous stem cells
(1,3*10 per scaffold) seeded on scaffold 5 days prior to surgery, 6.) SGS
scaffold+BMP-2 (1 mg / scaffold). Afterwards, the soft tissue were repositioned and
sutured. Bone volume to total volume (BV/TV) was analyzed using µCT. Results: The
µCT data showed significant less bone formation in the SGS scaffolds in all three
variations after both eight and twelve weeks. Due to lack of discrimination between
intertrabecular tissue and non-degraded scaffold on µCT, we were unable to quantify
the cause. The bioplotted scaffold showed significant higher bone volume to total
volume after 12 weeks compared to the empty defect without taking the existing
volume of bioplotted scaffold into account.
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