Organelle Isolation and Marker Enzyme Assay - Association for ...
Organelle Isolation and Marker Enzyme Assay - Association for ...
Organelle Isolation and Marker Enzyme Assay - Association for ...
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132 <strong>Organelle</strong> <strong>Isolation</strong><br />
mitochondria. Complete purification was finally achieved by feeding the cells Triton WR-1339, a<br />
very low-density compound that preferentially accumulated into the lysosomes. This, in effect,<br />
decreased the density of the lysosomes, allowing them to be easily separated from the mitochondria<br />
using density centrifugation.<br />
Figure 8.1. Schematic separation of organelles by differential centrifugation. See text <strong>for</strong><br />
details.