Integration of Conservation Strategies of Plant Genetic ... - Genres
Integration of Conservation Strategies of Plant Genetic ... - Genres Integration of Conservation Strategies of Plant Genetic ... - Genres
When regenerating germplasm a number of points must be taken into consideration: • Selection within the original population as a result of regeneration procedures should be minimized. • No contamination with other samples, both during regeneration and seed handling should be allowed. • The breeding system of the crop in question is important. Cross pollinating crops need additional measures to insure proper isolation. • The population size of the original sample should be sufficiently large to avoid genetic drift. Compared to self pollinating species, cross pollinating species usually require a higher number of plants for regenerations to maintain the genetic variation that exists within the population. To minimize the impact that regenerations can have on the genetic identity of a seed sample, the frequency of regeneration should be kept as low as possible. Plants that during regeneration clearly seem contaminants and not belonging to the total population are discarded. The reproduction rate should also be considered in order to produce sufficient seeds. So far only in the case of faba beans the reproduction rate is a limiting factor and a relatively high number of plants is used.
Table 2. Regeneration of crops Crop Breeding system Pollination mechanism Isolation Number of plants Onion and leek Mostly CP Insects Gauze cages 80 - 150 Barley SP - 1) ± 200 Beets Mostly CP Wind Hemp/ Greenhouses 50 - 60 Cole crops Mostly CP Insects Gauze cages 80 - 150 Tomato SP - - 5 Pepper SP - - 5 Eggplant SP - - 5 Clover CP Insects Rye fields ± 50 Cocksfoot CP Wind Rye fields ± 50 Lettuce SP - - 8 - 16 Miscellaneous crucifers Mostly CP Insects Rye fields 50 - 100 Faba beans Often CP Insects Rye fields ≥ 100 Rye-grass CP Wind Rye fields ± 50 Lupin CP Insect Rye fields ≥ 100 Maize Mostly CP Wind Bagging 100 Oats SP - - ± 200 Peas SP - - ≥ 50 Spinach Mostly CP Wind Greenhouse compartments Timothy CP Wind Rye fields ± 50 Wheat SP - 1) ± 200 CP= Cross pollinating, SP= (Predominantly) self pollinating. 1) Only wild species in greenhouse. A very important aspect of regeneration is the production of healthy, viable seeds. In general, regenerations under glass yield better quality seeds compared to accessions regenerated in the field. Germplasm that is distributed by genebanks, must be carefully checked for the presence of seedborne pathogens and pests, so that it does not contribute to the spreading of diseases and pests. It should provide an uncontaminated basic stock for breeding programmes. CGN maintains a high standard of disease and pest control, including pathogens with no official quarantine status, such as Lactuca Mosaic Virus. 80
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Table 2. Regeneration <strong>of</strong> crops<br />
Crop Breeding system Pollination<br />
mechanism<br />
Isolation Number <strong>of</strong><br />
plants<br />
Onion and leek Mostly CP Insects Gauze cages 80 - 150<br />
Barley SP - 1) ± 200<br />
Beets Mostly CP Wind Hemp/ Greenhouses<br />
50 - 60<br />
Cole crops Mostly CP Insects Gauze cages 80 - 150<br />
Tomato SP - - 5<br />
Pepper SP - - 5<br />
Eggplant SP - - 5<br />
Clover CP Insects Rye fields ± 50<br />
Cocksfoot CP Wind Rye fields ± 50<br />
Lettuce SP - - 8 - 16<br />
Miscellaneous<br />
crucifers<br />
Mostly CP Insects Rye fields 50 - 100<br />
Faba beans Often CP Insects Rye fields ≥ 100<br />
Rye-grass CP Wind Rye fields ± 50<br />
Lupin CP Insect Rye fields ≥ 100<br />
Maize Mostly CP Wind Bagging 100<br />
Oats SP - - ± 200<br />
Peas SP - - ≥ 50<br />
Spinach Mostly CP Wind Greenhouse<br />
compartments<br />
Timothy CP Wind Rye fields ± 50<br />
Wheat SP - 1) ± 200<br />
CP= Cross pollinating, SP= (Predominantly) self pollinating.<br />
1) Only wild species in greenhouse.<br />
A very important aspect <strong>of</strong> regeneration is the production <strong>of</strong> healthy, viable seeds. In general,<br />
regenerations under glass yield better quality seeds compared to accessions regenerated in the<br />
field.<br />
Germplasm that is distributed by genebanks, must be carefully checked for the presence <strong>of</strong> seedborne<br />
pathogens and pests, so that it does not contribute to the spreading <strong>of</strong> diseases and pests.<br />
It should provide an uncontaminated basic stock for breeding programmes. CGN maintains a high<br />
standard <strong>of</strong> disease and pest control, including pathogens with no <strong>of</strong>ficial quarantine status, such<br />
as Lactuca Mosaic Virus.<br />
80