Integration of Conservation Strategies of Plant Genetic ... - Genres

Integration of Conservation Strategies of Plant Genetic ... - Genres Integration of Conservation Strategies of Plant Genetic ... - Genres

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Mediterranean agriculture. Thanks to numerous exploration missions, carried out in different regions of Italy, Mediterranean countries, Ethiopia and South Africa ( Perrino and Porceddu, 1990; Perrino et al, 1990; Perrino, 1991; Hammer et al, 1992 ), still relatively rich in genetic variability, the Germplasm Institute has collected and stores 11,802 accessions of seed samples ( Tab. 1 ). They represent several cereal and grain legume crops and wild relatives typical of the visited regions. If besides these samples, directly collected by the staff of the Institute in collaboration with other institutions and international organizations, one considers also those obtained through exchange activities with other genebanks and similar institutes of the world, the number of accessions stored at the Germplasm Institute grows up to 55,806 ( Tab. 2 ) . These genetic stocks represent more than 40 genera and more than 584 species. The high number of accessions, more than 30,000, of Triticum species, stored in Bari, may already indicate that the Germplasm Institute, together with the National Seed Storage Laboratory of ARS-USDA in Fort Collins (Colorado, USA), the Pansoviet Institute for Plant Industry N.I. Vavilov of the Academy of Science of CSI (St. Petersburg), and the Department of Genetics of the University of Kyoto ( Japan ), is responsible for the safeguard of the world collection of wheats. The information collected during exploration and characterization, as well as the one deriving from evaluation, is filed in special data bases of the Institute. The documentation service is a source of information on the stored material. It allows to check the amount of genetic erosion occurring in different areas, to decide about the opportunity of organizing further missions for collecting germplasm, to know the amount of material available for distribution, its germinability, and the need for rejuvenation. It is a source of information necessary for taking further decisions. In Italy, including the Germplasm Institute, there are at least 16 institutions that maintain seed germplasm collections ( Tab. 3 ). It is possible to count 55 genera, 137 species of crops and wild relatives, and 60,118 accessions. If one excludes the world collection of Triticum, stored at the Germplasm Institute, the rest of the collections, in most cases, are small or relatively small. In particular 14,243 accessions, stored by 15 institutions others than the Germplasm Institute, are maintained for breeding purposes and not always available for distribution. The effective total number of accessions stored as seed collections in Italy can be worked out by adding 14,243, shared by 15 institutions ( Tab. 3 ), to 55,806, stored at the Germplasm Institute ( Tab. 2). The result would be 69,049 accessions. Naturally here is not considered the number of samples of germplasm used and mantained by the several seed companies. 2.2 Field genebanks As far as perennial and vegetatively propagated crops, wood and fruit trees conservation are concerned, an important role has been played by institutes of several Universities, Ministry of Agriculture and Forestry ( MAF ), CNR and various other formal and informal organizations. In fact, since 1960, several institutions started identification, collection and conservation of old varieties of fruit trees. In 1980 a European Cooperative Programme for Conservation and Exchange of Crop Genetic Resources (ECP/GR) of IBPGR was started; it included European countries and Palestine. Taking into account the priorities established by IBPGR in 1981, the scientific Committee of ECP/GR considered most of the fruit species cultivated in Europe at the highest priority for conservation. In 1981 a national Working Group "Protection of Genetic Resources of Fruit Arboreal Species" was

Plant genetic resources activities in Italy founded by the CNR and coordinated by the Faculty of Agriculture of Florence. The Group, while interacting with the ECP/GR European Working Groups, brought together the major national fruit tree research institutions. Each of them takes care of the conservation of different species depending on the Region in which it is located. As a result of this activity, carried out for several years, today there are at least 18 Institutions which take care of the preservation of 14 genera of fruit crops and other perennials , including 46 species and 13,560 accessions (Tab. 4). In particular as far as fruit trees are concerned all over the Italian peninsula, there are, already, 43 stations, 6 of which for olive, pear, and apple trees, 5 for vines, 4 for peach trees, 3 for almond, cherry and citrus fruit, 2 for apricot, plum trees and other species. The more important and spread out the species, the higher the number of the stations; in some areas the same Institution takes care of the safeguard of different species located in different stations ( Perrino, 1990a ). It seems that this activity of creating other stations for field collections is still going on ( Tab. 6 ). Thus, a series of programmes organized in working groups has been carried out, in order to face the problem of genetic erosion and conservation in a global way. Within these initiatives, in Italy a range as wide as possible of genetic variability of fruit trees and their wild relatives has been identified for collection and conservation purposes. This material is conserved as field collections, because other methods of conservation do not guarantee their genetic stability and "in situ" conservation is not always possible. A methodology for the evaluation and documentation of accessions has been set up; information is stored in computers and is available for all potential users. Notwithstanding these efforts and the ones of IBPGR in coordinating the centres of collection and storage of fruit germplasm, the number of stations and field collections is thought to be insufficient to represent the variability still present in fruit tree species. In the future, it is desirable that the number of specialized stations be increased and studies on "in vitro" culture and cryoconservation promoted. 2.3 Botanic gardens The first botanic garden in Europe was founded in Italy in 1545. The model was soon imitated by the main Universities and Courts of Europe. They became the meeting centres of explorers and scientists with the aim of improving our knowledges about nature and man. Today, in the world, there are about 600 botanic gardens conserving, as field and greenhouses collections, only a small portion of the existing plant species. In Europe about 300 botanic gardens are conserving thousands of plant species. It is difficult to figure out the exact number of species due to the existence of several duplicates among botanic gardens. In Italy there are 36 botanic gardens ( Tab. 5; Fig.1), of which 27 belong to Universities of different Regions (Raimondo, 1992). Numerous field and greenhouses collections representing several thousands of species of different geographical origin are maintained on a limited surface. In fact the surface of each botanic garden ranges from ca. 1,000 to ca. 200,000 sq m, while the total surface all over the peninsula is 1,258,239 sq m ( = ca. 1,3 sq Km ). In the absence of detailed lists of species, maintained at each botanic garden, it is impossible to know the total number of species conserved over the total Italian surface dedicated to this kind of protection. One can only report that the number of species present in each botanic garden ranges from very few , say 10 to ca. 6,000 species. Usually, each botanic garden conserves native and exotic species.

<strong>Plant</strong> genetic resources activities in Italy<br />

founded by the CNR and coordinated by the Faculty <strong>of</strong> Agriculture <strong>of</strong> Florence. The Group, while<br />

interacting with the ECP/GR European Working Groups, brought together the major national fruit tree<br />

research institutions. Each <strong>of</strong> them takes care <strong>of</strong> the conservation <strong>of</strong> different species depending on the<br />

Region in which it is located. As a result <strong>of</strong> this activity, carried out for several years, today there are<br />

at least 18 Institutions which take care <strong>of</strong> the preservation <strong>of</strong> 14 genera <strong>of</strong> fruit crops and other<br />

perennials , including 46 species and 13,560 accessions (Tab. 4). In particular as far as fruit trees are<br />

concerned all over the Italian peninsula, there are, already, 43 stations, 6 <strong>of</strong> which for olive, pear, and<br />

apple trees, 5 for vines, 4 for peach trees, 3 for almond, cherry and citrus fruit, 2 for apricot, plum trees<br />

and other species. The more important and spread out the species, the higher the number <strong>of</strong> the stations;<br />

in some areas the same Institution takes care <strong>of</strong> the safeguard <strong>of</strong> different species located in different<br />

stations ( Perrino, 1990a ). It seems that this activity <strong>of</strong> creating other stations for field collections is still<br />

going on ( Tab. 6 ).<br />

Thus, a series <strong>of</strong> programmes organized in working groups has been carried out, in order to face the<br />

problem <strong>of</strong> genetic erosion and conservation in a global way.<br />

Within these initiatives, in Italy a range as wide as possible <strong>of</strong> genetic variability <strong>of</strong> fruit trees and their<br />

wild relatives has been identified for collection and conservation purposes. This material is conserved<br />

as field collections, because other methods <strong>of</strong> conservation do not guarantee their genetic stability and<br />

"in situ" conservation is not always possible.<br />

A methodology for the evaluation and documentation <strong>of</strong> accessions has been set up; information is<br />

stored in computers and is available for all potential users.<br />

Notwithstanding these efforts and the ones <strong>of</strong> IBPGR in coordinating the centres <strong>of</strong> collection and<br />

storage <strong>of</strong> fruit germplasm, the number <strong>of</strong> stations and field collections is thought to be insufficient to<br />

represent the variability still present in fruit tree species. In the future, it is desirable that the number <strong>of</strong><br />

specialized stations be increased and studies on "in vitro" culture and cryoconservation promoted.<br />

2.3 Botanic gardens<br />

The first botanic garden in Europe was founded in Italy in 1545. The model was soon imitated by the<br />

main Universities and Courts <strong>of</strong> Europe. They became the meeting centres <strong>of</strong> explorers and scientists<br />

with the aim <strong>of</strong> improving our knowledges about nature and man.<br />

Today, in the world, there are about 600 botanic gardens conserving, as field and greenhouses<br />

collections, only a small portion <strong>of</strong> the existing plant species. In Europe about 300 botanic gardens are<br />

conserving thousands <strong>of</strong> plant species. It is difficult to figure out the exact number <strong>of</strong> species due to the<br />

existence <strong>of</strong> several duplicates among botanic gardens.<br />

In Italy there are 36 botanic gardens ( Tab. 5; Fig.1), <strong>of</strong> which 27 belong to Universities <strong>of</strong> different<br />

Regions (Raimondo, 1992). Numerous field and greenhouses collections representing several thousands<br />

<strong>of</strong> species <strong>of</strong> different geographical origin are maintained on a limited surface. In fact the surface <strong>of</strong> each<br />

botanic garden ranges from ca. 1,000 to ca. 200,000 sq m, while the total surface all over the peninsula<br />

is 1,258,239 sq m ( = ca. 1,3 sq Km ). In the absence <strong>of</strong> detailed lists <strong>of</strong> species, maintained at each<br />

botanic garden, it is impossible to know the total number <strong>of</strong> species conserved over the total Italian<br />

surface dedicated to this kind <strong>of</strong> protection. One can only report that the number <strong>of</strong> species present in<br />

each botanic garden ranges from very few , say 10 to ca. 6,000 species. Usually, each botanic garden<br />

conserves native and exotic species.

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