note - FIZ Karlsruhe

Review the answers using IDENT and SCORE
=> D IDENT SCORE 1-7
Advanced similarity searching
L3 ANSWER 1 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
IDENT 100%
SCORE 496 100% of query self score 496
. . . .
Sequences with 100 % identity, and with
L3 ANSWER 5 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
IDENT 100%
SCORE 496 100% of query self score 496
L3 ANSWER 6 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
IDENT 100%
SCORE 60 12% of query self score 496
Sequences with areas of 100 % identity,
L3 ANSWER 7 OF 394 DGENE COPYRIGHT 2010 THOMSON but with REUTERS low overall on STN similarity.
IDENT 100%
SCORE 56 11% of query self score 496
Review answers in more detail using IDENT, SCORE and alignment (ALIGN)
=> D TRIAL IDENT SCORE ALIGN 1,6,7
L3 ANSWER 1 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
AN ASS06733 cDNA DGENE
TI Providing to a mammal a mammalian Interleukin (IL)-1 Receptor antagonist
(Ra) peptide or polypeptide, by introducing to cells or tissue of the
mammal, a composition comprising a recombinant self-complimentary adeno-
associated viral vector.
DESC Horse Interleukin-1 receptor antagonist coding sequence, SEQ ID 11.
KW ss; gene; IL1RA; Interleukin-1 receptor antagonist; antagonist; . . . .
therapeutic; gene therapy; protein therapy; prophylactic to disease; drug
delivery; vector; arthritis; antiarthritic; antiinflammatory;
osteoarthritis; rheumatoid arthritis; immunosuppressive; injury;
vulnerary; joint disease; periarticular joint disease; musculoskeletal-
gen.; neuromuscular disease; neuroprotective; autoimmune disease;
immunosuppressive.
SQL 534
IDENT 100%
SCORE 496 100% of query self score 496
BLASTALIGN
Query = 250 letters
Length = 534
Score = 496 bits (250), Expect = e-145
Identities = 250/250 (100%)
Strand = Plus / Plus
100% overall similarity.
A sequence hit with 100 % identity, and
with 100% overall similarity.
Query: 1 ggaagagaccctgcaagatgcaagccttcagaatctgggatgttaaccagaagaccttct
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 86 ggaagagaccctgcaagatgcaagccttcagaatctgggatgttaaccagaagaccttct
Note: this ALIGN display has
been truncated for brevity, as
Query: 61 acatgaggaataaccaactagttgctggatacttgcaagaatcaaatactaaat indicated by “. . .”.
. . .
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 146 acatgaggaataaccaactagttgctggatacttgcaagaatcaaatactaaat . . .
GENESEQ on STN (DGENE) Workshop Manual | Page 71