note - FIZ Karlsruhe
note - FIZ Karlsruhe
note - FIZ Karlsruhe
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Review the answers using IDENT and SCORE<br />
=> D IDENT SCORE 1-7<br />
Advanced similarity searching<br />
L3 ANSWER 1 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN<br />
IDENT 100%<br />
SCORE 496 100% of query self score 496<br />
. . . .<br />
Sequences with 100 % identity, and with<br />
L3 ANSWER 5 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN<br />
IDENT 100%<br />
SCORE 496 100% of query self score 496<br />
L3 ANSWER 6 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN<br />
IDENT 100%<br />
SCORE 60 12% of query self score 496<br />
Sequences with areas of 100 % identity,<br />
L3 ANSWER 7 OF 394 DGENE COPYRIGHT 2010 THOMSON but with REUTERS low overall on STN similarity.<br />
IDENT 100%<br />
SCORE 56 11% of query self score 496<br />
Review answers in more detail using IDENT, SCORE and alignment (ALIGN)<br />
=> D TRIAL IDENT SCORE ALIGN 1,6,7<br />
L3 ANSWER 1 OF 394 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN<br />
AN ASS06733 cDNA DGENE<br />
TI Providing to a mammal a mammalian Interleukin (IL)-1 Receptor antagonist<br />
(Ra) peptide or polypeptide, by introducing to cells or tissue of the<br />
mammal, a composition comprising a recombinant self-complimentary adeno-<br />
associated viral vector.<br />
DESC Horse Interleukin-1 receptor antagonist coding sequence, SEQ ID 11.<br />
KW ss; gene; IL1RA; Interleukin-1 receptor antagonist; antagonist; . . . .<br />
therapeutic; gene therapy; protein therapy; prophylactic to disease; drug<br />
delivery; vector; arthritis; antiarthritic; antiinflammatory;<br />
osteoarthritis; rheumatoid arthritis; immunosuppressive; injury;<br />
vulnerary; joint disease; periarticular joint disease; musculoskeletal-<br />
gen.; neuromuscular disease; neuroprotective; autoimmune disease;<br />
immunosuppressive.<br />
SQL 534<br />
IDENT 100%<br />
SCORE 496 100% of query self score 496<br />
BLASTALIGN<br />
Query = 250 letters<br />
Length = 534<br />
Score = 496 bits (250), Expect = e-145<br />
Identities = 250/250 (100%)<br />
Strand = Plus / Plus<br />
100% overall similarity.<br />
A sequence hit with 100 % identity, and<br />
with 100% overall similarity.<br />
Query: 1 ggaagagaccctgcaagatgcaagccttcagaatctgggatgttaaccagaagaccttct<br />
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br />
Sbjct: 86 ggaagagaccctgcaagatgcaagccttcagaatctgggatgttaaccagaagaccttct<br />
Note: this ALIGN display has<br />
been truncated for brevity, as<br />
Query: 61 acatgaggaataaccaactagttgctggatacttgcaagaatcaaatactaaat indicated by “. . .”.<br />
. . .<br />
||||||||||||||||||||||||||||||||||||||||||||||||||||||<br />
Sbjct: 146 acatgaggaataaccaactagttgctggatacttgcaagaatcaaatactaaat . . .<br />
GENESEQ on STN (DGENE) Workshop Manual | Page 71