note - FIZ Karlsruhe

Advanced similarity searching
Similarity
Score
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The graphic representation gives a count of
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hit sequences (x-axis) and similarity score (y-
248 |||||||||||||||||||||||||| axis). The graph gives a visual clue about the
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Answer Count 80 160 240 320 400
ENTER EITHER THE NUMBER OF ANSWERS YOU WISH TO KEEP
OR ENTER MINIMUM PERCENT OF SELF SCORE FOLLOWED BY %
(BEST ANSWER PERCENTAGE OF SELF SCORE IS 100%)
ENTER (ALL) OR ? :ALL
L1 RUN STATEMENT CREATED
L2 394 GGAAGAGACCCTGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAG
AAGACCTTCTACATGAGGAATAACCAACTAGTTGCTGGATACTTGCAAGA
ATCAAATACTAAATTACAAGAGAAGATAGATGTGGTGCCCATTGAGCCTG
ATGCTCTATTCCTGGGACTCCATGGGAGGAAGCTGTGCCTGGCCTGTGTC
AAGTCTGGTGATGAGATTAGGTTCCAATTGGAGGCAGTTAACATCACTGA
/SQN.-F F
Sort into in descending IDENT and descending SCORE order (L3)
Answer set arranged by accession number; to sort by descending
similarity score, enter at an arrow prompt (=>) "sor score d".
=> SOR L2 IDENT D SCORE D
PROCESSING COMPLETED FOR L2
L3 394 SOR L2 IDENT D SCORE D
In this example, ALL of
the results are kept (L2).
Page 70 | GENESEQ on STN (DGENE) Workshop Manual
The initial results (L2) are sorted into
descending (highest first) percent identity
(IDENT) and descending BLAST score (L3).