note - FIZ Karlsruhe

Run a BLAST similarity search with default settings
=> RUN BLAST GSSFLSPEHQR/SQP
BLAST Version 2.2
The BLAST software is used herein with permission of the
National Center for Biotechnology Information (NCBI) of
the National Library of Medicine (NLM). See also, Altschul,
Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui
Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein
database search programs." Nucleic Acids Res. 25:3389-3402
BLAST SEARCHING
. . . .
Lambda K H
0.311 0.127 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,048,764
Number of Sequences: 6631376
Number of extensions: 127418
Number of successful extensions: 1295
Number of sequences better than 10.0: 0
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1295
Number of HSP's gapped (non-prelim): 0
length of query: 11
length of database: 1,256,955,012
effective HSP length: 0
effective length of query: 24
effective length of database: 1,256,955,012
effective search space: 30166920288
effective search space used: 30166920288
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 70 (31.6 bits)
NO ANSWERS FOUND BELOW EXPECTATION VALUE OF 10.0
Helpful
HINT
Advanced similarity searching
The BLAST summary gives the BLAST parameters. In this
example, the default values are used (page 137). These are:
c) Matrix: BLOSUM-62
d) Gap penalties: 11 for opening and 1 for extending a gap
For this peptide sequence query,
no answer sequences are found
using the default BLAST settings.
NCBI recommended settings 1 for short amino acid sequence queries (