note - FIZ Karlsruhe
note - FIZ Karlsruhe
note - FIZ Karlsruhe
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Run a BLAST similarity search with default settings<br />
=> RUN BLAST GSSFLSPEHQR/SQP<br />
BLAST Version 2.2<br />
The BLAST software is used herein with permission of the<br />
National Center for Biotechnology Information (NCBI) of<br />
the National Library of Medicine (NLM). See also, Altschul,<br />
Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui<br />
Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),<br />
"Gapped BLAST and PSI-BLAST: a new generation of protein<br />
database search programs." Nucleic Acids Res. 25:3389-3402<br />
BLAST SEARCHING<br />
. . . .<br />
Lambda K H<br />
0.311 0.127 0.369<br />
Gapped<br />
Lambda K H<br />
0.267 0.0410 0.140<br />
Matrix: BLOSUM62<br />
Gap Penalties: Existence: 11, Extension: 1<br />
Number of Hits to DB: 29,048,764<br />
Number of Sequences: 6631376<br />
Number of extensions: 127418<br />
Number of successful extensions: 1295<br />
Number of sequences better than 10.0: 0<br />
Number of HSP's better than 10.0 without gapping: 0<br />
Number of HSP's successfully gapped in prelim test: 0<br />
Number of HSP's that attempted gapping in prelim test: 1295<br />
Number of HSP's gapped (non-prelim): 0<br />
length of query: 11<br />
length of database: 1,256,955,012<br />
effective HSP length: 0<br />
effective length of query: 24<br />
effective length of database: 1,256,955,012<br />
effective search space: 30166920288<br />
effective search space used: 30166920288<br />
T: 11<br />
A: 40<br />
X1: 16 ( 7.2 bits)<br />
X2: 38 (14.6 bits)<br />
X3: 64 (24.7 bits)<br />
S1: 42 (21.8 bits)<br />
S2: 70 (31.6 bits)<br />
NO ANSWERS FOUND BELOW EXPECTATION VALUE OF 10.0<br />
Helpful<br />
HINT<br />
Advanced similarity searching<br />
The BLAST summary gives the BLAST parameters. In this<br />
example, the default values are used (page 137). These are:<br />
c) Matrix: BLOSUM-62<br />
d) Gap penalties: 11 for opening and 1 for extending a gap<br />
For this peptide sequence query,<br />
no answer sequences are found<br />
using the default BLAST settings.<br />
NCBI recommended settings 1 for short amino acid sequence queries (