note - FIZ Karlsruhe

Advanced similarity searching
SCORE 62 100% of query self score 62
BLASTALIGN
Query = 31 letters
Length = 6474
Score = 61.9 bits (31), Expect = 2e-14
Identities = 31/31 (100%)
Strand = Plus / Plus
Query: 1 aaaatgtttttaaagaagccagctcaagcaa 31
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Sbjct: 3298 aaaatgtttttaaagaagccagctcaagcaa 3328
L2 ANSWER 20 OF 540 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
AN AXQ61701 cDNA DGENE
TI Generating cell clones with stable transgene (Gene of Interest (GOI))
expression comprises inactivation of endogenous essential genes of cell
substrate and trans-complementation of essential genes products.
DESC Human endogenous cell essential target cDNA sequence, SEQ:171.
KW coding sequence; gene expression; gene silencing; protein production; rna
interference; ss.
SQL 7185
SCORE 62 100% of query self score 62
BLASTALIGN
Query = 31 letters
Length = 7185
Score = 61.9 bits (31), Expect = 3e-14
Identities = 31/31 (100%)
Strand = Plus / Plus
Query: 1 aaaatgtttttaaagaagccagctcaagcaa 31
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Sbjct: 3292 aaaatgtttttaaagaagccagctcaagcaa 3322
Run a BLAST similarity search with altered settings
=> RUN BLAST AAAATGTTTTTAAAGAAGCCAGCTCAAGCAA/SQN -W 7 -F F -E 1000
BLAST Version 2.2
The BLAST search is adjusted with the NCBI
The BLAST software is used herein with permission
recommended
of the
settings
National
for short
Center
nucleotide
for
Biotechnology Information (NCBI) of the National
sequence
Library
queries
of
(see
Medicine
Helpful HINT
(NLM).
below).
. . . .
Helpful
HINT
Page 54 | GENESEQ on STN (DGENE) Workshop Manual
(continued on next page)
NCBI recommended settings 1 for short nucleotide sequence queries (