note - FIZ Karlsruhe

Sort similarity score descending
Answer set arranged by accession number; to sort by descending
similarity score, enter at an arrow prompt (=>) "sor score d".
=> SOR SCORE D
PROCESSING COMPLETED FOR L2
L3 58 SOR L2 SCORE D
Review using a free-of-charge format with alignment
=> D TRI ALIGN 1-58
Introduction to similarity searching
L3 ANSWER 1 OF 58 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
AN AAI67779 DNA DGENE
TI Novel ErbB-4 ligand, referred as neuregulin (NRG)-4 and polynucleotide
sequences encoding NRG-4, useful for upregulating or downregulating
ErbB-4 receptor activity to treat Alzheimer's disease, stroke, gastric
cancer -
DESC Mouse pro-NRG-4 nucleotide sequence.
KW ErbB-4; neuregulin-4; NRG-4; pro-NRG-4; neuroprotective; vulnerary;
cerebroprotective; vasotropic; antiparkinsonian; anticonvulsant;
cytostatic; nootropic; mouse; ds.
SQL 801
BLASTALIGN
Query = 348 letters
Length = 801
Plus / Plus indicates that the query sequence is
aligned to the single strand of the hit sequence.
Plus / Minus indicates an alignment between query
Score = 690 bits (348), Expect = 0.0
sequence and complementary hit sequence strand.
Identities = 348/348 (100%)
Strand = Plus / Plus
Query: 1 atgccaacagatcacgagcagccctgtggtcccaggcacaggtcattttgcctcaatggg
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 115 atgccaacagatcacgagcagccctgtggtcccaggcacaggtcattttgcctcaatggg
The ALIGN display:
� First line: portion of query with
Query: 61 gggatttgttatgtgatccctactatccccagcccattctgtaggtgcattgaaaattac
similarity
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 175 gggatttgttatgtgatccctactatccccagcccattctgtaggtgcattgaaaattac
� Second line: similarity (baridentical
residues, blank-no match)
Query: 121 accggagcacgctgcgaagaggtttttctcccaagctccagcatcccaagcgaaagtaat
� Third line: portion of retrieved
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(subject) sequence with similarity
Sbjct: 235 accggagcacgctgcgaagaggtttttctcccaagctccagcatcccaagcgaaagtaat
Query: 181 ctgtcggcagctttcgtggtgctggcggtcctcctcactcttaccatcgcggcgctctgc
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 295 ctgtcggcagctttcgtggtgctggcggtcctcctcactcttaccatcgcggcgctctgc
Query: 241 ttcctgtgcaggaagggccaccttcagagggccagttcagtccagtgtgagatcagcctg
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 355 ttcctgtgcaggaagggccaccttcagagggccagttcagtccagtgtgagatcagcctg
Query: 301 gtagagacaaacaataccagaacccgtcacagccacagagaacactga 348
||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 415 gtagagacaaacaataccagaacccgtcacagccacagagaacactga 462
. . . .
GENESEQ on STN (DGENE) Workshop Manual | Page 29