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note - FIZ Karlsruhe

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Appendices<br />

II: Nucleotide Codes<br />

The following table lists the symbols and ambiguity codes for nucleotides according to the IUPAC<br />

system that may be used in DGENE nucleic acid sequence searches using RUN GETSEQ (page<br />

102). Uncommon, modified or unknown nucleotides are typically represented by the parent<br />

nucleotide or by an 'N'. Details about uncommon nucleotides in a sequence will typically be found<br />

in the feature table (FEAT).<br />

Codes Name or Definition<br />

--------- ------------------<br />

A Adenine<br />

G Guanine<br />

U Uracil<br />

R A or G<br />

S C or G<br />

K G or T/U<br />

H A, C or T/U; not G<br />

B C, G or T/U; not A<br />

C Cytosine<br />

T Thymine<br />

M A or C<br />

W A or T/U<br />

Y C or T/U<br />

V A, C or G; not T/U<br />

D A, G or T/U; not C<br />

N Unknown or Other<br />

<strong>note</strong><br />

Exact and Subsequence Searches of Nucleic Acids (/SQEN and /SQSN) both<br />

allow all codes to be used, and match the codes in the query exactly against the<br />

codes in the database.<br />

Page 126 | GENESEQ on STN (DGENE) Workshop Manual

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