note - FIZ Karlsruhe

Excluding residues
Sequence Code Match Searching
An approach similar to that for alternate residues (page 117) is used to exclude residues.
Search Question: Find polypeptides with the following subsequence where X is any
amino acid except H or N: PTGKXEA
Search Strategy
To find protein sequences in DGENE…
Step 1 Subsequence search in DGENE
Step 2 Display sequence information
Run the subsequence search with excluded residues in square brackets
=> RUN GETSEQ PTGK[-HN]EA/SQSP
RUN GETSEQ AT 18:30:32 ON 30 DEC 2010
COPYRIGHT (C) 2010 FIZ KARLSRUHE GMBH
L1 RUN STATEMENT CREATED
L1 127 PTGK[-HN]EA/SQSP
Review answers retrieved in a free-of-charge format, including GETSEQ ALIGN (HIT)
=> D TRI ALIGN 100
L1 ANSWER 100 OF 127 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN
AN AAR25654 Protein DGENE
TI New mutant somatotropin cpds. - having mutation in alpha-helix region and
opt. alpha-helix 3 region to increase solubility while maintaining
activity
DESC Recombinant porcine somatotropin mutant I122L.
KW ST; mutation; alpha helix; soluble; stability; sustained release; growth;
feed efficiency; lean; consumption; food.
SQL 193
SEQ
ptgkdea
=======
HITS AT: 63-69
This answer is a hit for PTGKDEA.
GENESEQ on STN (DGENE) Workshop Manual | Page 119