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note - FIZ Karlsruhe

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Nucleic acid EXACT Search<br />

Sequence Code Match Searching<br />

Searching of nucleic acids is similar to polypeptide sequence searching, except that there are no<br />

FAMILY search options available for nucleic acids. However nucleotide ambiguity codes can be<br />

used to specify base alternatives (see page 126).<br />

Search Question: Find patent references for the following nucleotide sequence:<br />

GACCAGCCCCAAGATGACTATC<br />

Search Strategy<br />

To find nucleic acid sequences in DGENE…<br />

Step 1 Nucleic acid EXACT search<br />

Step 2 Display sequence and patent family details<br />

Nucleic acid exact search<br />

=> RUN GETSEQ GACCAGCCCCAAGATGACTATC/SQEN<br />

RUN GETSEQ AT 18:26:43 ON 30 DEC 2010<br />

COPYRIGHT (C) 2010 <strong>FIZ</strong> KARLSRUHE GMBH<br />

L1 RUN STATEMENT CREATED<br />

L1 1 GACCAGCCCCAAGATGACTATC/SQEN<br />

Review answers retrieved in a free-of-charge format, including GETSEQ ALIGN (HIT)<br />

=> D TRI ALIGN<br />

L1 ANSWER 1 OF 1 DGENE COPYRIGHT 2010 THOMSON REUTERS on STN<br />

AN AAV64044 DNA DGENE<br />

TI Obtaining genomic and diagnostic marker from single collection step - by<br />

placing sample on bibulous solid support and extracting diagnostic and<br />

genomic marker<br />

DESC Human chemokine receptor 5 gene PCR primer #2.<br />

KW Human; chemokine receptor 5; CKR5; PCR primer; diagnosis; serum based<br />

assay; detection; genomic information; ss.<br />

SQL 22<br />

SEQ<br />

gaccagcccc aagatgacta tc<br />

========== ========== ==<br />

HITS AT: 1-22<br />

The ALIGN display shows the<br />

area of the hit sequence which is<br />

a match to the query.<br />

GENESEQ on STN (DGENE) Workshop Manual | Page 113

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