Catalytic Synthesis and Characterization of Biodegradable ...
Catalytic Synthesis and Characterization of Biodegradable ...
Catalytic Synthesis and Characterization of Biodegradable ...
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Chapter 1<br />
experiments with <strong>and</strong> without applying electrical potentials, the dopped electroactive<br />
copolymers had the ability <strong>of</strong> improving the differentiation <strong>of</strong> PC-12 cells, as shown in Figure<br />
1.5.11. They also prepared a new kind <strong>of</strong> water-soluble electroactive polymer, aniline<br />
pentamer cross-linked chitosan. These new polymers showed good electroactivity even in<br />
aqueous solution. The MTT assay, cell adhesion test, <strong>and</strong> degradation assessment in the<br />
presence <strong>of</strong> enzyme confirmed that these polymers had good biocompatibility <strong>and</strong><br />
biodegradability. The electroactive polymers can obviously improve the neuronal<br />
differentiation <strong>of</strong> PC-12 cells even without the extra electrical stimulation, as shown in<br />
Figure1.5.12.<br />
Figure 1.5.12 Visualization <strong>of</strong> PC-12 neurite outgrowth by micrographs for the substrates (A)<br />
without electroactivity (chitosan), (B) with electroactivity (aniline pentamer cross-linked<br />
chitosan) on day 5. 139<br />
A B<br />
As the study progressing, Chen et al. found that the oligomers without high conductivity<br />
<strong>and</strong> the polymers containing oligomers also showed improvement in the C6 cell<br />
differentiation in the absence <strong>of</strong> electrical stimulation, as shown in Figure 1.5.13. In the<br />
culture medium, the only difference from the electroactive polymers may be the exchange <strong>of</strong><br />
the ion between the medium with polymer, <strong>and</strong> between the polymer with cells, which means<br />
the electroactivity changed the ion exchange between the cells <strong>and</strong> medium.<br />
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